Basic Statistics
Measure | Value |
---|---|
Filename | SRR3549504_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 1152524 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT | 2689 | 0.233314013417508 | TruSeq Adapter, Index 21 (95% over 22bp) |
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2648 | 0.22975660376703652 | No Hit |
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC | 2643 | 0.22932277332185705 | TruSeq Adapter, Index 15 (95% over 22bp) |
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC | 2461 | 0.21353134511732508 | TruSeq Adapter, Index 15 (95% over 22bp) |
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC | 2399 | 0.20815184759709993 | TruSeq Adapter, Index 15 (95% over 22bp) |
TGTTTTGGGACAACCATCCGTAACAAGATCTGACTCCCTCTTCTGGAGTGT | 1222 | 0.1060281608018575 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACGCATT | 30 | 2.1655069E-6 | 45.000004 | 12 |
CGTGCAA | 20 | 7.0332934E-4 | 45.000004 | 16 |
ATTACGA | 20 | 7.0332934E-4 | 45.000004 | 13 |
GCGATAG | 35 | 1.211938E-7 | 45.000004 | 9 |
GACGCAT | 35 | 1.211938E-7 | 45.000004 | 11 |
TCGAATC | 25 | 3.8908132E-5 | 45.0 | 34 |
CGTAACA | 300 | 0.0 | 42.75 | 19 |
CGCAATC | 95 | 0.0 | 42.63158 | 18 |
TCCGTAA | 315 | 0.0 | 42.142857 | 17 |
CTTTACG | 60 | 3.6379788E-12 | 41.250004 | 1 |
CGATAGG | 55 | 6.184564E-11 | 40.909092 | 2 |
AGACACG | 665 | 0.0 | 40.601505 | 24 |
AACACGT | 625 | 0.0 | 40.32 | 41 |
TTTACGG | 230 | 0.0 | 40.1087 | 2 |
TACGGGA | 215 | 0.0 | 39.76744 | 4 |
CCGTAAC | 335 | 0.0 | 39.626865 | 18 |
GTCAAGC | 695 | 0.0 | 39.496403 | 16 |
ACACGAC | 685 | 0.0 | 39.416058 | 26 |
CGAACGT | 40 | 3.4582263E-7 | 39.375004 | 18 |
CAACGAC | 115 | 0.0 | 39.130436 | 10 |