FastQCFastQC Report
Sat 18 Jun 2016
SRR3549504_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549504_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1152524
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGCT26890.233314013417508TruSeq Adapter, Index 21 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26480.22975660376703652No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC26430.22932277332185705TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC24610.21353134511732508TruSeq Adapter, Index 15 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC23990.20815184759709993TruSeq Adapter, Index 15 (95% over 22bp)
TGTTTTGGGACAACCATCCGTAACAAGATCTGACTCCCTCTTCTGGAGTGT12220.1060281608018575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATT302.1655069E-645.00000412
CGTGCAA207.0332934E-445.00000416
ATTACGA207.0332934E-445.00000413
GCGATAG351.211938E-745.0000049
GACGCAT351.211938E-745.00000411
TCGAATC253.8908132E-545.034
CGTAACA3000.042.7519
CGCAATC950.042.6315818
TCCGTAA3150.042.14285717
CTTTACG603.6379788E-1241.2500041
CGATAGG556.184564E-1140.9090922
AGACACG6650.040.60150524
AACACGT6250.040.3241
TTTACGG2300.040.10872
TACGGGA2150.039.767444
CCGTAAC3350.039.62686518
GTCAAGC6950.039.49640316
ACACGAC6850.039.41605826
CGAACGT403.4582263E-739.37500418
CAACGAC1150.039.13043610