FastQCFastQC Report
Sat 18 Jun 2016
SRR3549503_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549503_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615273
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51340.31784100891923533No Hit
GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC25670.15892050445961767Illumina PCR Primer Index 5 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC24880.1540296903371752Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT24290.1503770570052245Illumina PCR Primer Index 5 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC19290.11942253724293045Illumina PCR Primer Index 5 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGA207.0341374E-445.043
TCGTACA700.041.78571734
ATGCGAT403.4593904E-739.37524
TTCGTAC750.039.033
TTTGGGA75800.038.766494
CATGCGA356.249862E-638.5714323
CACGACC7550.038.14569527
CGTTTTT30550.038.0769271
TTGGGAC29150.037.8216135
TTTGGGC44400.037.8040544
CCGTATC301.1401265E-437.50000438
ACACGAC7700.037.1103926
GCGATAT850.037.0588239
TGGGCGA29750.036.9831926
TTGGGAG48750.036.9230775
TTGGGAT33450.036.5246625
ACACGCG6600.036.13636436
AACACGT7350.036.12244841
AACCGTA250.002107360636.033
ATTACGA250.002107360636.014
Produced by FastQC (version 0.11.5)