##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549503_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1615273 Sequences flagged as poor quality 0 Sequence length 51 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.23273527137518 33.0 31.0 34.0 30.0 34.0 2 32.41721492280252 34.0 31.0 34.0 30.0 34.0 3 32.485230050895424 34.0 31.0 34.0 30.0 34.0 4 35.961431287466574 37.0 35.0 37.0 35.0 37.0 5 35.13754145584059 37.0 35.0 37.0 35.0 37.0 6 34.823531378287136 37.0 35.0 37.0 32.0 37.0 7 35.62616350301157 37.0 35.0 37.0 33.0 37.0 8 35.91658499832536 37.0 35.0 37.0 35.0 37.0 9 37.78010156797024 39.0 38.0 39.0 35.0 39.0 10 37.28984264579424 39.0 37.0 39.0 34.0 39.0 11 37.19396040174014 39.0 37.0 39.0 34.0 39.0 12 36.97680268289014 39.0 37.0 39.0 33.0 39.0 13 36.921997705650995 39.0 37.0 39.0 33.0 39.0 14 38.087465091040336 40.0 37.0 41.0 33.0 41.0 15 38.1782608884071 40.0 37.0 41.0 33.0 41.0 16 38.171033008042606 40.0 37.0 41.0 33.0 41.0 17 38.09133626328181 40.0 37.0 41.0 33.0 41.0 18 38.04281876809679 40.0 37.0 41.0 33.0 41.0 19 38.03156865743438 40.0 37.0 41.0 34.0 41.0 20 37.95831664368809 40.0 36.0 41.0 33.0 41.0 21 37.8412200290601 40.0 36.0 41.0 33.0 41.0 22 37.861486572238874 40.0 36.0 41.0 33.0 41.0 23 37.780964579981216 40.0 36.0 41.0 33.0 41.0 24 37.69685062525034 40.0 36.0 41.0 33.0 41.0 25 37.57741570619951 39.0 35.0 41.0 33.0 41.0 26 37.54410740475449 39.0 35.0 41.0 33.0 41.0 27 37.50171023721687 39.0 35.0 41.0 33.0 41.0 28 37.40212149896643 39.0 35.0 41.0 33.0 41.0 29 37.33354547497544 39.0 35.0 41.0 32.0 41.0 30 37.240125972513624 39.0 35.0 41.0 32.0 41.0 31 37.1396104559415 39.0 35.0 41.0 32.0 41.0 32 37.109329506529235 39.0 35.0 41.0 32.0 41.0 33 36.99506708773068 39.0 35.0 41.0 31.0 41.0 34 36.827810531099075 39.0 35.0 41.0 31.0 41.0 35 36.67587212811704 39.0 35.0 41.0 31.0 41.0 36 36.55613199750135 39.0 35.0 41.0 30.0 41.0 37 36.551887513751545 39.0 35.0 41.0 31.0 41.0 38 36.44720923336179 39.0 35.0 41.0 30.0 41.0 39 36.39681589427917 39.0 35.0 41.0 30.0 41.0 40 36.260004346014576 38.0 35.0 40.0 30.0 41.0 41 36.13288403879716 38.0 35.0 40.0 30.0 41.0 42 36.07383519689861 38.0 35.0 40.0 30.0 41.0 43 35.95040590661764 38.0 35.0 40.0 30.0 41.0 44 35.85675734070959 38.0 35.0 40.0 29.0 41.0 45 35.82379820624749 38.0 35.0 40.0 29.0 41.0 46 35.609401630560285 38.0 34.0 40.0 29.0 41.0 47 35.51798983825025 38.0 34.0 40.0 28.0 41.0 48 35.44401534601272 37.0 34.0 40.0 28.0 41.0 49 35.46506070490871 37.0 34.0 40.0 29.0 41.0 50 35.27971123147604 37.0 34.0 40.0 28.0 41.0 51 34.2408979782365 36.0 33.0 39.0 25.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 9.0 9 21.0 10 31.0 11 42.0 12 26.0 13 24.0 14 46.0 15 59.0 16 121.0 17 238.0 18 424.0 19 739.0 20 1260.0 21 2051.0 22 3149.0 23 4575.0 24 6617.0 25 9899.0 26 13468.0 27 16777.0 28 19644.0 29 23512.0 30 28426.0 31 35458.0 32 45969.0 33 64894.0 34 112113.0 35 138720.0 36 144339.0 37 209797.0 38 324373.0 39 408333.0 40 119.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.840568745964305 4.326265591017741 53.54110419724716 17.292061465770804 2 21.172086699895313 4.246155293872924 52.758264392458734 21.823493613773028 3 21.379543891342205 4.661317312924812 51.39044607320249 22.568692722530496 4 19.206165149792017 4.874284408889395 50.34888839224082 25.570662049077775 5 17.664630065629773 8.063033307682355 49.296063266085675 24.976273360602203 6 22.66960445695557 7.8371272224571324 51.62607187763307 17.867196442954224 7 84.84497666957846 2.185574822336534 8.801422422092116 4.168026085992895 8 87.12279596080663 2.67614205152937 6.564525005989699 3.636536981674305 9 81.38475663247019 5.142226731951813 8.572916157206862 4.900100478371148 10 41.04433120593237 27.802854378176324 15.226032998756247 15.92678141713506 11 32.922050947424985 24.443236530295497 23.021433528573805 19.613278993705705 12 28.97113986304482 20.971191866638023 29.115821288413784 20.94184698190337 13 25.97177071615758 21.909113815435532 30.652094104216438 21.46702136419045 14 21.76944702226806 23.844514209053205 30.981264467368675 23.40477430131006 15 20.827439076861932 22.672328454694654 34.1603555559958 22.339876912447618 16 23.70367114413477 20.636697326086672 32.60309557579431 23.056535953984252 17 23.195336020598376 20.688948555445428 30.618848949991733 25.496866473964463 18 24.443607984532644 21.084052045691347 30.031765528180067 24.440574441595942 19 25.678755232087703 23.79108670794349 28.04553781311271 22.484620246856103 20 29.251711630170256 23.009361265866513 26.86171315932353 20.8772139446397 21 28.841502334280335 23.458820892815023 25.761775254090175 21.937901518814467 22 26.07695417430985 23.077770754541184 25.86429662354289 24.98097844760607 23 25.386173111294504 24.415810825786107 26.290416542590634 23.907599520328763 24 25.49612356549017 23.00366563423025 27.731782800802097 23.76842799947749 25 25.931529840466595 23.130888710453277 25.66884978576377 25.26873166331636 26 24.576217147194313 24.032841507287003 26.155516745466556 25.235424600052127 27 22.991407644404383 23.604369044737332 27.99984894194356 25.404374368914727 28 22.267505245243374 25.120273786536394 28.12199547692557 24.49022549129466 29 23.61959866846038 24.221106896481277 27.39413089923499 24.765163535823355 30 25.683088864854426 23.194778839242655 27.096224601042675 24.025907694860248 31 25.314296716406453 25.70692384507139 25.271517570094964 23.707261868427196 32 25.798734950686352 25.1233073294731 25.671945237739997 23.40601248210055 33 26.142268211008297 24.185199653557017 25.571652593710166 24.100879541724527 34 24.19405264620903 24.823048487778845 27.36874819302991 23.614150672982216 35 24.0597719394802 24.383494307154272 27.4981380856363 24.058595667729232 36 23.773999813034703 26.882947959880465 26.171551186703425 23.171501040381408 37 23.757779644679257 27.19620769987488 26.754672429985522 22.291340225460342 38 23.694260970127033 26.236431860125194 27.217442500431815 22.85186466931596 39 24.34114852411945 24.7358186510887 27.26987945690914 23.653153367882705 40 24.759591722266144 23.959850749687515 28.698987725294735 22.58156980275161 41 21.54663638901907 24.704306949970686 28.868370857434005 24.880685803576238 42 22.62694912872313 25.03793476396869 27.243939569348342 25.091176537959836 43 22.8197338778027 23.7100477752058 27.929829818241252 25.540388528750245 44 23.739640296098553 24.014330704469153 27.37673445912858 24.869294540303713 45 24.358606873265387 23.363171426749535 26.070887088436447 26.20733461154864 46 23.54728891029566 24.54365299240438 27.08743351743018 24.82162457986978 47 22.296292948622305 25.349089596619272 28.531028501064526 23.823588953693893 48 22.383708512431024 23.20982273584713 29.343584644824745 25.062884106897098 49 22.75361502359044 23.720634220964506 29.149995078231356 24.375755677213697 50 22.01324481991589 23.75697482716544 28.61411043210652 25.615669920812145 51 21.269717255225586 23.409293661195353 27.894665483791286 27.42632359978778 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 327.0 1 1173.0 2 2019.0 3 6083.0 4 10147.0 5 6982.5 6 3818.0 7 3638.5 8 3459.0 9 3478.0 10 3497.0 11 3563.5 12 3630.0 13 3713.5 14 3797.0 15 3741.5 16 3686.0 17 3597.5 18 3509.0 19 3502.5 20 3496.0 21 3731.5 22 3967.0 23 4613.5 24 5260.0 25 6369.0 26 8848.0 27 10218.0 28 12720.5 29 15223.0 30 17183.5 31 19144.0 32 22810.5 33 26477.0 34 30200.0 35 33923.0 36 36318.5 37 38714.0 38 44556.0 39 50398.0 40 56964.5 41 63531.0 42 72205.0 43 80879.0 44 93848.0 45 106817.0 46 118465.0 47 130113.0 48 131423.5 49 132734.0 50 126070.0 51 119406.0 52 111630.5 53 103855.0 54 100251.0 55 96647.0 56 97231.5 57 97816.0 58 96712.0 59 95608.0 60 93257.5 61 90907.0 62 81451.5 63 71996.0 64 63140.0 65 54284.0 66 45780.5 67 37277.0 68 32565.5 69 27854.0 70 23264.5 71 18675.0 72 16271.5 73 13868.0 74 11536.5 75 7243.5 76 5282.0 77 4262.5 78 3243.0 79 2476.5 80 1710.0 81 1280.0 82 850.0 83 570.0 84 290.0 85 221.5 86 153.0 87 108.5 88 64.0 89 39.0 90 14.0 91 10.5 92 7.0 93 4.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1615273.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.31886735177074 #Duplication Level Percentage of deduplicated Percentage of total 1 77.34508469627572 23.450153632178566 2 9.153091660190551 5.550227438078325 3 3.0083282913397134 2.736273192471237 4 1.3994148409320788 1.697146917292762 5 0.8292498254397038 1.2570957729492709 6 0.5408836680028024 0.9839388109749702 7 0.41766924845717485 0.8864280978620801 8 0.3546829237589783 0.8602867613909342 9 0.2976030665709438 0.8120689108960178 >10 6.096369428765786 47.760015305177866 >50 0.4824824015653907 8.779717231949109 >100 0.0714844930271978 3.747569672874312 >500 0.0026400513201073894 0.5833929928688654 >1k 8.12323483109966E-4 0.5795755400395387 >5k 2.030808707774915E-4 0.3161097229961747 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5134 0.31784100891923533 No Hit GCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC 2567 0.15892050445961767 Illumina PCR Primer Index 5 (95% over 23bp) TCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC 2488 0.1540296903371752 Illumina PCR Primer Index 5 (95% over 23bp) CTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGCT 2429 0.1503770570052245 Illumina PCR Primer Index 5 (95% over 24bp) CCTGTCTCTTATACACATCTGACGCGCATGATTTCGTATGCCGTCTTCTGC 1929 0.11942253724293045 Illumina PCR Primer Index 5 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.095451976229405E-4 0.0 0.0 0.16201595643584707 0.0 2 3.095451976229405E-4 0.0 0.0 0.6439778291347654 0.0 3 3.095451976229405E-4 0.0 0.0 0.9477654860819192 0.0 4 3.095451976229405E-4 0.0 0.0 1.3110477300122023 0.0 5 3.095451976229405E-4 0.0 0.0 1.9386815727124764 0.0 6 3.095451976229405E-4 0.0 0.0 2.506325556113425 0.0 7 3.095451976229405E-4 0.0 0.0 2.983953796045622 0.0 8 3.095451976229405E-4 0.0 0.0 3.738624987850351 0.0 9 3.095451976229405E-4 0.0 0.0 4.218048589928761 0.0 10 3.714542371475286E-4 0.0 0.0 4.970862510547753 0.0 11 4.333632766721167E-4 0.0 0.0 6.112712835539256 0.0 12 4.333632766721167E-4 0.0 0.0 7.071126676419404 0.0 13 4.952723161967048E-4 0.0 0.0 7.390948774603427 0.0 14 4.952723161967048E-4 0.0 0.0 7.503747044617226 0.0 15 4.952723161967048E-4 0.0 0.0 7.684830985226646 0.0 16 4.952723161967048E-4 0.0 0.0 8.085444379990255 0.0 17 4.952723161967048E-4 0.0 0.0 8.650859637968319 0.0 18 4.952723161967048E-4 0.0 0.0 9.277441026996675 0.0 19 4.952723161967048E-4 0.0 0.0 9.69309831836476 0.0 20 4.952723161967048E-4 0.0 0.0 10.15159666508386 0.0 21 4.952723161967048E-4 0.0 0.0 10.767405881234938 0.0 22 4.952723161967048E-4 0.0 0.0 11.42512751714416 0.0 23 6.19090395245881E-4 0.0 0.0 12.068981528199878 0.0 24 6.19090395245881E-4 0.0 0.0 12.581402648344893 0.0 25 6.809994347704691E-4 0.0 0.0 13.051106531217943 0.0 26 6.809994347704691E-4 0.0 0.0 13.445095658752422 0.0 27 6.809994347704691E-4 0.0 0.0 13.8504141405199 0.0 28 6.809994347704691E-4 0.0 0.0 14.255113531892132 0.0 29 6.809994347704691E-4 0.0 0.0 14.710949789911675 0.0 30 6.809994347704691E-4 0.0 0.0 15.220646912317608 0.0 31 7.429084742950572E-4 0.0 0.0 15.68267407428961 0.0 32 7.429084742950572E-4 0.0 0.0 16.16426449275138 0.0 33 7.429084742950572E-4 0.0 0.0 16.620781750205694 0.0 34 7.429084742950572E-4 0.0 0.0 17.09395253929212 0.0 35 7.429084742950572E-4 0.0 0.0 17.594858578085564 0.0 36 7.429084742950572E-4 0.0 0.0 18.07521081575684 0.0 37 7.429084742950572E-4 0.0 0.0 18.557544142692908 0.0 38 7.429084742950572E-4 0.0 0.0 19.026381299012613 0.0 39 7.429084742950572E-4 0.0 0.0 19.524687158146023 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGCGA 20 7.0341374E-4 45.0 43 TCGTACA 70 0.0 41.785717 34 ATGCGAT 40 3.4593904E-7 39.375 24 TTCGTAC 75 0.0 39.0 33 TTTGGGA 7580 0.0 38.76649 4 CATGCGA 35 6.249862E-6 38.57143 23 CACGACC 755 0.0 38.145695 27 CGTTTTT 3055 0.0 38.076927 1 TTGGGAC 2915 0.0 37.821613 5 TTTGGGC 4440 0.0 37.804054 4 CCGTATC 30 1.1401265E-4 37.500004 38 ACACGAC 770 0.0 37.11039 26 GCGATAT 85 0.0 37.058823 9 TGGGCGA 2975 0.0 36.983192 6 TTGGGAG 4875 0.0 36.923077 5 TTGGGAT 3345 0.0 36.524662 5 ACACGCG 660 0.0 36.136364 36 AACACGT 735 0.0 36.122448 41 AACCGTA 25 0.0021073606 36.0 33 ATTACGA 25 0.0021073606 36.0 14 >>END_MODULE