FastQCFastQC Report
Sat 18 Jun 2016
SRR3549502_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549502_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1006403
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33390.33177564057340847No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGC18510.1839223452235337No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC17000.16891841538628163No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC16880.167726050101202No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCG15950.15848521914183483No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT11970.1189384371866936No Hit
TCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC10980.10910142358478661No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA207.032866E-445.00000411
TTACACG1600.042.18750434
TTACGGG2800.040.178573
CGATGAA1750.039.85714319
TCGATTG403.4576442E-739.3750041
TTTGGGC28200.038.856384
CGAGATA2100.038.5714319
CGTTTTT20400.038.3823551
TTGGGAT21900.037.808225
TGGGCGA20250.037.6666686
TGACGTA301.1398211E-437.49999620
CGTAGCG301.1398211E-437.49999630
GGACGTA301.1398211E-437.4999968
TCGAACG301.1398211E-437.49999629
TTGGGAC18150.036.942155
TTTGGGA42800.036.7990654
TGTTTCG2700.036.6666641
TACGAAT1350.036.66666412
GCTACGA1350.036.66666410
CTTGTTG6050.036.4462781
Produced by FastQC (version 0.11.5)