##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549502_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1006403
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.24858729554661	33.0	31.0	34.0	30.0	34.0
2	32.45384204935796	34.0	31.0	34.0	31.0	34.0
3	32.512390165768586	34.0	31.0	34.0	30.0	34.0
4	35.97694362993751	37.0	35.0	37.0	35.0	37.0
5	35.269803448519134	37.0	35.0	37.0	35.0	37.0
6	34.889795638526515	37.0	35.0	37.0	32.0	37.0
7	35.63391305471069	37.0	35.0	37.0	35.0	37.0
8	35.88207805421884	37.0	35.0	37.0	35.0	37.0
9	37.7503683911912	39.0	38.0	39.0	35.0	39.0
10	37.28498921406236	39.0	37.0	39.0	34.0	39.0
11	37.21135668315774	39.0	37.0	39.0	34.0	39.0
12	36.990665767093304	39.0	37.0	39.0	33.0	39.0
13	36.9248919170551	39.0	37.0	39.0	33.0	39.0
14	38.06490143610462	40.0	37.0	41.0	33.0	41.0
15	38.155605656978366	40.0	37.0	41.0	33.0	41.0
16	38.15101505063081	40.0	37.0	41.0	33.0	41.0
17	38.072626969514204	40.0	37.0	41.0	33.0	41.0
18	38.01723464655809	40.0	37.0	41.0	33.0	41.0
19	38.00371421786302	40.0	37.0	41.0	34.0	41.0
20	37.89757184746071	40.0	36.0	41.0	33.0	41.0
21	37.80339088814322	40.0	36.0	41.0	33.0	41.0
22	37.82115613725317	40.0	36.0	41.0	33.0	41.0
23	37.72310694622333	40.0	36.0	41.0	33.0	41.0
24	37.646260990875426	40.0	35.0	41.0	33.0	41.0
25	37.544439950993784	39.0	35.0	41.0	33.0	41.0
26	37.47408642462314	39.0	35.0	41.0	33.0	41.0
27	37.419066715818616	39.0	35.0	41.0	33.0	41.0
28	37.2999663156807	39.0	35.0	41.0	32.0	41.0
29	37.224545236848456	39.0	35.0	41.0	32.0	41.0
30	37.1241808698901	39.0	35.0	41.0	32.0	41.0
31	37.041659255785206	39.0	35.0	41.0	32.0	41.0
32	36.981090080216376	39.0	35.0	41.0	32.0	41.0
33	36.83414894430958	39.0	35.0	41.0	31.0	41.0
34	36.66989665173892	39.0	35.0	41.0	31.0	41.0
35	36.51405649625448	39.0	35.0	41.0	30.0	41.0
36	36.40026410891065	39.0	35.0	41.0	30.0	41.0
37	36.34572134622015	39.0	35.0	41.0	30.0	41.0
38	36.21164285082616	38.0	35.0	40.0	30.0	41.0
39	36.192170532083075	38.0	35.0	40.0	30.0	41.0
40	36.07921280043879	38.0	35.0	40.0	30.0	41.0
41	35.936307821022	38.0	35.0	40.0	29.0	41.0
42	35.82951064335062	38.0	35.0	40.0	29.0	41.0
43	35.69390691402947	38.0	35.0	40.0	29.0	41.0
44	35.60803077892256	38.0	35.0	40.0	29.0	41.0
45	35.59443980194813	38.0	34.0	40.0	29.0	41.0
46	35.37413044277491	37.0	34.0	40.0	28.0	41.0
47	35.25907017367794	37.0	34.0	40.0	28.0	41.0
48	35.161239582950365	37.0	34.0	40.0	27.0	41.0
49	35.19688335587235	37.0	34.0	40.0	28.0	41.0
50	35.017574470664336	37.0	34.0	40.0	27.0	41.0
51	33.946326670329874	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	18.0
10	36.0
11	22.0
12	16.0
13	25.0
14	34.0
15	36.0
16	74.0
17	144.0
18	265.0
19	510.0
20	904.0
21	1518.0
22	2338.0
23	3331.0
24	4639.0
25	6732.0
26	9092.0
27	11184.0
28	12871.0
29	15067.0
30	17998.0
31	23093.0
32	29665.0
33	41221.0
34	70839.0
35	88777.0
36	90804.0
37	129481.0
38	198769.0
39	246784.0
40	109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.843836912250858	4.632438496308139	55.020006895845896	19.503717695595103
2	20.422931966617746	5.689271593983722	52.546941930816985	21.340854508581554
3	20.648388369271554	5.418107855401861	51.72093087957806	22.212572895748522
4	18.7789583298142	4.91214751943307	51.47480681198287	24.834087338769855
5	18.77240032074626	7.542008519449962	48.71487863211855	24.970712527685233
6	22.54643517557082	7.929427873327087	51.96695558339949	17.5571813677026
7	82.95752298035679	2.310505831163063	10.516363723081112	4.215607465399049
8	85.6851579337502	3.424572462522469	7.3188374835925565	3.5714321201347774
9	79.93497634645365	5.006344376954361	9.137095179565243	5.921584097026738
10	41.50583811852707	26.009560782310864	16.0613591175702	16.423241981591868
11	32.524346608664715	23.220419652962086	23.777154877320516	20.47807886105268
12	28.32910871688578	20.262856927095804	30.24832000699521	21.159714349023204
13	25.120950553605265	20.985430289854065	32.43809885304396	21.455520303496712
14	21.435647548745386	23.43266067370626	32.48460109916206	22.64709067838629
15	20.556973697415447	21.72499485792471	35.35233897355234	22.365692471107497
16	22.93405325699546	20.270806028996336	32.952803201103336	23.84233751290487
17	22.13675833637221	20.630006071126576	30.7662039958148	26.467031596686418
18	23.693291852269915	21.4747968756055	30.487886065522456	24.344025206602126
19	24.83955234632647	23.48323683454839	28.30009449494884	23.3771163241763
20	28.098386034222873	22.69508338111075	27.986403061199145	21.220127523467237
21	27.364584565030114	24.28778531065587	26.820269812391256	21.52736031192276
22	25.5818991000623	22.330915150292675	27.611503542815353	24.475682206829667
23	25.168148346139667	23.9318642730596	26.682949077059586	24.217038303741145
24	24.46505028303771	22.59104950998755	28.142801641092085	24.801098565882654
25	24.229359411686968	23.38973552344339	26.612698889013647	25.768206175855994
26	22.517023498538855	24.325642908457148	27.27783999054057	25.879493602463427
27	22.678191539572122	23.278348733062202	28.534096182145717	25.509363545219955
28	21.135668315774097	23.96167340518659	30.2586538394659	24.64400443957341
29	23.059947158345114	22.894804566361586	28.251505609581844	25.79374266571145
30	24.475483479282158	22.80905362960961	28.55009374972054	24.165369141387693
31	24.540765478640267	24.369859787778854	26.341038331563006	24.748336402017877
32	25.7443588701544	24.69875387891332	25.363100070250187	24.19378718068209
33	25.347201866449126	23.491484027770188	25.87373050358554	25.287583602195145
34	23.244465686211193	23.371055133977144	27.698645572399922	25.68583360741174
35	22.894208383719047	23.996152634680143	26.9152615801026	26.19437740149821
36	23.89370858393705	25.745253144118212	26.014330243451184	24.346708028493556
37	24.50489515631412	25.06709538822917	27.06092887242983	23.367080583026876
38	23.35674675055619	24.99088337375783	26.549503528904424	25.102866346781553
39	23.523479162919823	23.86926509559292	26.540362061718813	26.066893679768445
40	24.003803645259403	23.61638429138228	27.600275436380855	24.779536626977464
41	20.90991382179902	24.533710650703544	28.046518144321908	26.50985738317553
42	22.268912155468534	24.88595522867082	27.39866633942864	25.446466276432005
43	22.5274566947833	23.156131291341538	28.212853101590518	26.10355891228464
44	23.17759386647297	23.05358787682469	27.67251289990193	26.096305356800407
45	23.177891957794245	23.027157113005426	26.371344282558777	27.423606646641552
46	24.17848515952357	24.209884112030664	26.820667267486286	24.790963460959475
47	22.208101525929475	24.003207462616864	28.76392459084482	25.02476642060884
48	21.95243853605365	23.220320289188326	29.26491673812578	25.562324436632245
49	22.5783309469467	22.84154558362803	29.173203974948404	25.406919494476867
50	22.12523213861644	22.204723157621746	28.68035965711549	26.989685046646322
51	20.83111834921001	22.921135966407093	27.830799391496246	28.416946292886646
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	276.0
1	993.0
2	1710.0
3	4257.0
4	6804.0
5	4742.5
6	2681.0
7	2627.5
8	2574.0
9	2508.0
10	2442.0
11	2555.0
12	2668.0
13	2525.5
14	2383.0
15	2505.0
16	2627.0
17	2535.0
18	2443.0
19	2464.0
20	2485.0
21	2752.0
22	3019.0
23	3293.0
24	3567.0
25	4499.0
26	6212.5
27	6994.0
28	8396.0
29	9798.0
30	11516.5
31	13235.0
32	15805.5
33	18376.0
34	20325.5
35	22275.0
36	24367.5
37	26460.0
38	28547.5
39	30635.0
40	33484.5
41	36334.0
42	40016.0
43	43698.0
44	48211.0
45	52724.0
46	54178.0
47	55632.0
48	66351.5
49	77071.0
50	82032.0
51	86993.0
52	86064.0
53	85135.0
54	80009.0
55	74883.0
56	70161.5
57	65440.0
58	62260.5
59	59081.0
60	55504.0
61	51927.0
62	47626.5
63	43326.0
64	38253.0
65	33180.0
66	27934.5
67	22689.0
68	19075.5
69	15462.0
70	13621.0
71	11780.0
72	10216.5
73	8653.0
74	7457.5
75	4801.0
76	3340.0
77	2696.5
78	2053.0
79	1523.5
80	994.0
81	747.5
82	501.0
83	370.5
84	240.0
85	149.5
86	59.0
87	47.5
88	36.0
89	29.0
90	22.0
91	12.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1006403.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.251391825699955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.4728687750937	24.98607846726582
2	9.126956529431087	5.887141024136251
3	2.996468298759789	2.8992081958977156
4	1.366954480981712	1.7634473829615003
5	0.7846692684627286	1.2653338015388405
6	0.54097845538429	1.0468384880316408
7	0.43879699417561635	0.990626965376803
8	0.3779587995520412	0.9751757870659257
9	0.324471986010861	0.9418205841569383
>10	6.216040904730606	49.08055530873397
>50	0.30053444801226503	5.884546395687435
>100	0.0499885641881101	2.7754133383491775
>500	0.0012045437153680651	0.2929155134698198
>1k	0.0021079515018941138	1.2108987473281545
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3339	0.33177564057340847	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGC	1851	0.1839223452235337	No Hit
CCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1700	0.16891841538628163	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTC	1688	0.167726050101202	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCG	1595	0.15848521914183483	No Hit
CTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGCT	1197	0.1189384371866936	No Hit
TCTGTCTCTTATACACATCTGACGCCGTCGCATTCGTATGCCGTCTTCTGC	1098	0.10910142358478661	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.936377375663625E-5	0.0	0.0	0.12947099720489705	0.0
2	9.936377375663625E-5	0.0	0.0	0.5526613096344108	0.0
3	9.936377375663625E-5	0.0	0.0	0.8468774437278108	0.0
4	9.936377375663625E-5	0.0	0.0	1.259833287460391	0.0
5	9.936377375663625E-5	0.0	0.0	2.0240400714226805	0.0
6	9.936377375663625E-5	0.0	0.0	2.521852577943428	0.0
7	9.936377375663625E-5	0.0	0.0	3.019068901821636	0.0
8	9.936377375663625E-5	0.0	0.0	3.7571430132859303	0.0
9	9.936377375663625E-5	0.0	0.0	4.2181909235167225	0.0
10	9.936377375663625E-5	0.0	0.0	5.082158936330675	0.0
11	1.987275475132725E-4	0.0	0.0	6.356996153628318	0.0
12	1.987275475132725E-4	0.0	0.0	7.672870609487452	0.0
13	2.980913212699088E-4	0.0	0.0	8.02680437160859	0.0
14	2.980913212699088E-4	0.0	0.0	8.15528173107592	0.0
15	2.980913212699088E-4	0.0	0.0	8.375769945041897	0.0
16	2.980913212699088E-4	0.0	0.0	8.827875115634592	0.0
17	2.980913212699088E-4	0.0	0.0	9.470261912971246	0.0
18	2.980913212699088E-4	0.0	0.0	10.152592947358066	0.0
19	2.980913212699088E-4	0.0	0.0	10.669682025987601	0.0
20	2.980913212699088E-4	0.0	0.0	11.190149472924862	0.0
21	2.980913212699088E-4	0.0	0.0	11.848434474062577	0.0
22	2.980913212699088E-4	0.0	0.0	12.51804694540855	0.0
23	2.980913212699088E-4	0.0	0.0	13.152087185749645	0.0
24	2.980913212699088E-4	0.0	0.0	13.66977244702172	0.0
25	3.97455095026545E-4	0.0	0.0	14.118101794211663	0.0
26	3.97455095026545E-4	0.0	0.0	14.512476612251753	0.0
27	3.97455095026545E-4	0.0	0.0	14.904963518590465	0.0
28	3.97455095026545E-4	0.0	0.0	15.307287438531086	0.0
29	3.97455095026545E-4	0.0	0.0	15.743196314001448	0.0
30	3.97455095026545E-4	0.0	0.0	16.253429292241776	0.0
31	3.97455095026545E-4	0.0	0.0	16.70970774133225	0.0
32	3.97455095026545E-4	0.0	0.0	17.15396317379817	0.0
33	3.97455095026545E-4	0.0	0.0	17.601994429666842	0.0
34	3.97455095026545E-4	0.0	0.0	18.049230775345464	0.0
35	3.97455095026545E-4	0.0	0.0	18.534523446372873	0.0
36	3.97455095026545E-4	0.0	0.0	18.99020571282081	0.0
37	3.97455095026545E-4	0.0	0.0	19.450558076635303	0.0
38	3.97455095026545E-4	0.0	0.0	19.921144909146733	0.0
39	3.97455095026545E-4	0.0	0.0	20.399482116011182	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCATA	20	7.032866E-4	45.000004	11
TTACACG	160	0.0	42.187504	34
TTACGGG	280	0.0	40.17857	3
CGATGAA	175	0.0	39.857143	19
TCGATTG	40	3.4576442E-7	39.375004	1
TTTGGGC	2820	0.0	38.85638	4
CGAGATA	210	0.0	38.57143	19
CGTTTTT	2040	0.0	38.382355	1
TTGGGAT	2190	0.0	37.80822	5
TGGGCGA	2025	0.0	37.666668	6
TGACGTA	30	1.1398211E-4	37.499996	20
CGTAGCG	30	1.1398211E-4	37.499996	30
GGACGTA	30	1.1398211E-4	37.499996	8
TCGAACG	30	1.1398211E-4	37.499996	29
TTGGGAC	1815	0.0	36.94215	5
TTTGGGA	4280	0.0	36.799065	4
TGTTTCG	270	0.0	36.666664	1
TACGAAT	135	0.0	36.666664	12
GCTACGA	135	0.0	36.666664	10
CTTGTTG	605	0.0	36.446278	1
>>END_MODULE