##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549501_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1143318
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.221977612527745	33.0	31.0	34.0	30.0	34.0
2	32.407485056650906	34.0	31.0	34.0	30.0	34.0
3	32.48427559086798	34.0	31.0	34.0	30.0	34.0
4	35.95471950935785	37.0	35.0	37.0	35.0	37.0
5	35.15470761415459	37.0	35.0	37.0	35.0	37.0
6	34.82933706982659	37.0	35.0	37.0	32.0	37.0
7	35.64486170951564	37.0	35.0	37.0	33.0	37.0
8	35.90979062692969	37.0	35.0	37.0	35.0	37.0
9	37.79389636129231	39.0	38.0	39.0	35.0	39.0
10	37.30763881964598	39.0	37.0	39.0	34.0	39.0
11	37.18771417925721	39.0	37.0	39.0	34.0	39.0
12	37.025913175511974	39.0	37.0	39.0	33.0	39.0
13	36.97492298730537	39.0	37.0	39.0	33.0	39.0
14	38.133476425631365	40.0	37.0	41.0	33.0	41.0
15	38.22519893852804	40.0	37.0	41.0	33.0	41.0
16	38.233184468363135	40.0	37.0	41.0	34.0	41.0
17	38.16985563071691	40.0	37.0	41.0	33.0	41.0
18	38.13185395489269	40.0	37.0	41.0	33.0	41.0
19	38.12326929165814	40.0	37.0	41.0	34.0	41.0
20	38.01704775049461	40.0	37.0	41.0	34.0	41.0
21	37.91397144101641	40.0	36.0	41.0	33.0	41.0
22	37.960786937667386	40.0	36.0	41.0	33.0	41.0
23	37.87049272380912	40.0	36.0	41.0	33.0	41.0
24	37.8110709356452	40.0	36.0	41.0	33.0	41.0
25	37.699700345835545	40.0	36.0	41.0	33.0	41.0
26	37.67136352265949	40.0	36.0	41.0	33.0	41.0
27	37.604830851958944	40.0	36.0	41.0	33.0	41.0
28	37.51951687981821	39.0	36.0	41.0	33.0	41.0
29	37.47425213282744	39.0	36.0	41.0	33.0	41.0
30	37.393066495935514	39.0	35.0	41.0	32.0	41.0
31	37.32522010499266	39.0	35.0	41.0	32.0	41.0
32	37.2831626896454	39.0	35.0	41.0	32.0	41.0
33	37.15873361566948	39.0	35.0	41.0	32.0	41.0
34	37.01859062832913	39.0	35.0	41.0	31.0	41.0
35	36.9178819890879	39.0	35.0	41.0	31.0	41.0
36	36.789461899489034	39.0	35.0	41.0	31.0	41.0
37	36.7374553711216	39.0	35.0	41.0	31.0	41.0
38	36.62555212110716	39.0	35.0	41.0	31.0	41.0
39	36.58584313375631	39.0	35.0	41.0	31.0	41.0
40	36.48120645349763	39.0	35.0	41.0	30.0	41.0
41	36.341746565697385	39.0	35.0	40.0	30.0	41.0
42	36.29274532544751	38.0	35.0	40.0	30.0	41.0
43	36.191371954259445	38.0	35.0	40.0	30.0	41.0
44	36.13051836846792	38.0	35.0	40.0	30.0	41.0
45	36.130181629258004	38.0	35.0	40.0	30.0	41.0
46	35.913747531308	38.0	35.0	40.0	30.0	41.0
47	35.83912349844925	38.0	35.0	40.0	30.0	41.0
48	35.78489886453288	38.0	35.0	40.0	29.0	41.0
49	35.85153824220383	38.0	35.0	40.0	30.0	41.0
50	35.659437706744754	38.0	35.0	40.0	29.0	41.0
51	34.665635457501764	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	16.0
10	31.0
11	25.0
12	18.0
13	17.0
14	31.0
15	38.0
16	69.0
17	114.0
18	248.0
19	394.0
20	819.0
21	1418.0
22	2060.0
23	2904.0
24	4318.0
25	6276.0
26	8796.0
27	10802.0
28	12485.0
29	14758.0
30	18539.0
31	24213.0
32	31737.0
33	44076.0
34	74469.0
35	96717.0
36	101264.0
37	147754.0
38	232645.0
39	306124.0
40	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.198167089121313	4.281660920233916	54.55227679438267	16.967895196262106
2	19.468686752067228	5.694478701463635	53.53891043436734	21.297924112101796
3	20.91430380698983	5.686431946317647	51.85976255075141	21.539501695941112
4	18.248728700151666	4.8430095563963835	52.89578227579729	24.012479467654668
5	18.504125711306916	7.7905709522635	49.621015325569964	24.08428801085962
6	21.573350546392167	8.258594721678483	52.5942913520123	17.573763379917047
7	83.42482144075402	2.1307282838195496	10.141972749488769	4.3024775259376655
8	85.84908135794241	3.5862288532149416	6.677669729681505	3.8870200591611432
9	80.33976548956633	5.502493619447957	8.376322248053473	5.781418642932238
10	41.085332339734	28.474580125564366	15.589363589132683	14.85072394556895
11	30.2843128508429	23.580316237477238	25.660839766364212	20.47453114531565
12	26.25997316582088	21.408741924818816	30.885020615436826	21.446264293923477
13	24.469832540028232	22.70339485602431	32.643411544294764	20.183361059652697
14	21.159292515293206	25.139899835391375	30.85738176080495	22.843425888510456
15	19.924028135654297	22.339541579857922	35.05603865241341	22.680391632074365
16	21.66335175340544	21.57440012315034	32.37576947096083	24.386478652483387
17	21.857086130018068	20.8984726908874	30.887644557332255	26.356796621762275
18	23.405124383592316	20.97797813031895	30.99338941571811	24.623508070370622
19	24.26341577758769	23.664719701780257	28.754379796347123	23.31748472428493
20	26.381199281389776	23.653874075279145	28.830036787665374	21.134889855665705
21	25.424684995775454	25.41445162238327	27.4400473009259	21.72081608091537
22	24.18198611409949	22.919170344558555	27.040595879711503	25.858247661630447
23	22.731296104845722	24.862199318124965	27.33290300686248	25.073601570166833
24	23.470548001518388	22.91567175536465	29.229925532529005	24.383854710587954
25	22.347675799733757	25.001268238582792	27.739614000654235	24.911441961029215
26	21.693439620473043	24.68455845180431	27.887954182475916	25.73404774524673
27	21.92670805497683	24.276273092875297	29.071789300964383	24.725229551183485
28	20.22140821713644	24.627094124294377	30.787759835846195	24.363737822722985
29	22.543334400403037	22.706631051028673	29.19730118829582	25.552733360272473
30	23.164159052861933	24.24889663243297	29.17692190624131	23.41002240846379
31	24.085250122887945	26.471812741511986	25.95568337068077	23.4872537649193
32	24.402484698045512	26.628549537399042	25.361185601906033	23.607780162649412
33	23.889504057488818	24.832286380517058	26.618053769817323	24.660155792176806
34	21.836269524314318	25.277306925982096	27.936059783892148	24.95036376581144
35	22.256974874881706	24.226680591051657	27.872735319482416	25.643609214584217
36	22.462779384213317	26.12151649847199	27.274389102594377	24.141315014720313
37	22.609370271438042	27.790605938155437	27.723170631442873	21.876853158963648
38	23.37993454139618	27.848595054044456	26.236532618221702	22.534937786337657
39	24.206038914807603	25.546435899723434	27.18351324828263	23.06401193718633
40	23.706790236836994	25.0443883503977	27.940695414574073	23.30812599819123
41	19.994524707911534	26.288049344101992	28.20186509789927	25.515560850087205
42	20.69905310683467	26.06658864812764	28.026848173474043	25.207510071563643
43	21.830584316874223	23.89282771722303	28.872019857992264	25.404568107910485
44	22.6828406445101	23.28346094437418	28.862923526088103	25.170774885027612
45	23.31897162469234	23.654923652037315	26.68732583585669	26.33877888741365
46	23.121563729426107	24.89604816857602	27.985477356256087	23.99691074574178
47	20.078228454375775	25.357512083252427	30.451807808501226	24.112451653870576
48	20.294616196018957	24.29507800979255	30.100112129783664	25.310193664404828
49	21.76857182341221	23.724283182806534	30.471312443257258	24.035832550524
50	22.045047834460753	23.49836178560995	28.73776149767606	25.71882888225323
51	20.855527508532184	23.879620543016028	27.752383851212	27.51246809723979
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	148.0
1	874.0
2	1600.0
3	3663.0
4	5726.0
5	4021.5
6	2317.0
7	2294.5
8	2272.0
9	2224.0
10	2176.0
11	2181.5
12	2187.0
13	2165.5
14	2144.0
15	2253.5
16	2363.0
17	2344.5
18	2326.0
19	2362.5
20	2399.0
21	2800.0
22	3201.0
23	3222.5
24	3244.0
25	4407.5
26	7303.5
27	9036.0
28	10476.5
29	11917.0
30	14175.0
31	16433.0
32	19287.0
33	22141.0
34	25583.0
35	29025.0
36	30960.5
37	32896.0
38	36471.5
39	40047.0
40	47102.0
41	54157.0
42	60196.5
43	66236.0
44	81354.0
45	96472.0
46	101068.0
47	105664.0
48	105125.5
49	104587.0
50	94872.0
51	85157.0
52	79315.0
53	73473.0
54	68502.5
55	63532.0
56	60788.0
57	58044.0
58	55467.0
59	52890.0
60	50313.0
61	47736.0
62	42417.5
63	37099.0
64	33777.0
65	30455.0
66	26515.5
67	22576.0
68	18955.5
69	15335.0
70	13337.5
71	11340.0
72	9315.5
73	7291.0
74	6322.5
75	4347.5
76	3341.0
77	2469.0
78	1597.0
79	1300.0
80	1003.0
81	808.0
82	613.0
83	375.5
84	138.0
85	85.0
86	32.0
87	25.0
88	18.0
89	11.5
90	5.0
91	3.0
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1143318.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.64209934844432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.30331113624283	24.143969515882528
2	10.284487792358165	6.508455689473197
3	3.541822022863067	3.3621205296582355
4	1.5969786184634052	2.0212702441104167
5	0.8876356545197768	1.4043327782768087
6	0.5969618026148288	1.1333474799338892
7	0.41257281839844806	0.9138269075761959
8	0.34718875908799807	0.8788624966180427
9	0.27578891896609325	0.7853886335812662
>10	5.216174195764666	44.217373204166385
>50	0.4729304737434487	9.00170693072082
>100	0.05851138879189652	3.1019830982531773
>500	0.0032208103916749643	0.6677862586990108
>1k	0.0024156077937562232	1.8595762330501766
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2955	0.2584582766999207	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGC	2953	0.2582833472402254	No Hit
CTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGCT	2640	0.23090688679789872	No Hit
GCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	2568	0.22460942624886515	No Hit
TCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	2389	0.20895323960612883	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCG	2341	0.20475493257343974	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTC	2335	0.20423014419435362	No Hit
CCTGTCTCTTATACACATCTGACGCCTAGGAATTCGTATGCCGTCTTCTGC	2080	0.18192663808319295	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1635	0.14300483330097138	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.746472984768892E-5	0.0	0.0	0.24901208587637036	0.0
2	8.746472984768892E-5	0.0	0.0	0.9334235969345361	0.0
3	8.746472984768892E-5	0.0	0.0	1.335498960044362	0.0
4	8.746472984768892E-5	0.0	0.0	1.8560890320978065	0.0
5	8.746472984768892E-5	0.0	0.0	2.8339447117949685	0.0
6	8.746472984768892E-5	0.0	0.0	3.4269555801622995	0.0
7	8.746472984768892E-5	0.0	0.0	4.005359838645067	0.0
8	8.746472984768892E-5	0.0	0.0	4.920065983392197	0.0
9	8.746472984768892E-5	0.0	0.0	5.411617765136209	0.0
10	8.746472984768892E-5	0.0	0.0	6.49889182187283	0.0
11	8.746472984768892E-5	0.0	0.0	7.858618512085002	0.0
12	1.7492945969537784E-4	0.0	0.0	9.469194047500345	0.0
13	1.7492945969537784E-4	0.0	0.0	9.894709958209353	0.0
14	1.7492945969537784E-4	0.0	0.0	10.040076339216212	0.0
15	1.7492945969537784E-4	0.0	0.0	10.318913897970644	0.0
16	2.623941895430668E-4	0.0	0.0	10.85682198653393	0.0
17	2.623941895430668E-4	0.0	0.0	11.634645829069427	0.0
18	2.623941895430668E-4	0.0	0.0	12.482528920212925	0.0
19	2.623941895430668E-4	0.0	0.0	13.114199199172933	0.0
20	2.623941895430668E-4	0.0	0.0	13.707822320649198	0.0
21	2.623941895430668E-4	0.0	0.0	14.483109686019112	0.0
22	2.623941895430668E-4	0.0	0.0	15.326707005400072	0.0
23	2.623941895430668E-4	0.0	0.0	16.169867001131795	0.0
24	2.623941895430668E-4	0.0	0.0	16.803811363067844	0.0
25	2.623941895430668E-4	0.0	0.0	17.357113244084324	0.0
26	2.623941895430668E-4	0.0	0.0	17.833271233375143	0.0
27	2.623941895430668E-4	0.0	0.0	18.321674284844637	0.0
28	2.623941895430668E-4	0.0	0.0	18.843226468926407	0.0
29	2.623941895430668E-4	0.0	0.0	19.370114001528883	0.0
30	2.623941895430668E-4	0.0	0.0	19.995224425750315	0.0
31	2.623941895430668E-4	0.0	0.0	20.551937431230858	0.0
32	2.623941895430668E-4	0.0	0.0	21.100778611025103	0.0
33	2.623941895430668E-4	0.0	0.0	21.657841475425034	0.0
34	2.623941895430668E-4	0.0	0.0	22.184641543297666	0.0
35	2.623941895430668E-4	0.0	0.0	22.764532702187843	0.0
36	2.623941895430668E-4	0.0	0.0	23.295093753443922	0.0
37	2.623941895430668E-4	0.0	0.0	23.82915339389391	0.0
38	2.623941895430668E-4	0.0	0.0	24.355078814468065	0.0
39	2.623941895430668E-4	0.0	0.0	24.890712820055313	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGT	20	7.033273E-4	45.0	10
CGACACG	20	7.033273E-4	45.0	40
TTATCGA	20	7.033273E-4	45.0	13
GTTACGT	40	6.8157533E-9	45.0	9
ACGGATA	20	7.033273E-4	45.0	34
CGTACAT	35	1.2119199E-7	45.0	35
CGATAAC	35	1.2119199E-7	45.0	16
TGCGTAG	20	7.033273E-4	45.0	1
AGTCGAC	20	7.033273E-4	45.0	38
CGATCTA	30	2.1654923E-6	44.999996	10
CGAATAT	250	0.0	43.199997	14
TCGTTCA	235	0.0	42.12766	16
TCGTTTG	485	0.0	41.75258	1
ATTAGTC	105	0.0	40.714283	31
ATAGCGG	45	1.9283107E-8	40.0	2
CGTTCAT	250	0.0	39.6	17
ACGGCAT	40	3.458208E-7	39.375	40
CGATAGG	40	3.458208E-7	39.375	10
TACGTTG	40	3.458208E-7	39.375	1
ACACGCG	235	0.0	39.255318	36
>>END_MODULE