##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549498_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	62629
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.398968528956235	31.0	31.0	34.0	30.0	34.0
2	31.528924300244295	31.0	31.0	34.0	30.0	34.0
3	31.11871497229718	31.0	31.0	34.0	28.0	34.0
4	35.244678982579956	37.0	35.0	37.0	33.0	37.0
5	35.09158696450526	35.0	35.0	37.0	32.0	37.0
6	35.13455427996615	36.0	35.0	37.0	32.0	37.0
7	32.04410097558639	35.0	35.0	37.0	11.0	37.0
8	33.57821456513756	37.0	35.0	37.0	19.0	37.0
9	36.51833815005828	39.0	35.0	39.0	32.0	39.0
10	36.335723067588496	38.0	35.0	39.0	32.0	39.0
11	36.37776429449616	38.0	35.0	39.0	32.0	39.0
12	36.32668572067253	38.0	35.0	39.0	32.0	39.0
13	32.61885069217136	37.0	33.0	39.0	10.0	39.0
14	35.065640517970905	38.0	34.0	40.0	18.0	41.0
15	36.418464289706044	38.0	34.0	40.0	30.0	41.0
16	37.04649603218956	38.0	35.0	40.0	32.0	41.0
17	37.18580849127401	39.0	36.0	40.0	32.0	41.0
18	37.100304970540805	39.0	36.0	40.0	32.0	41.0
19	37.05280301457791	39.0	36.0	40.0	32.0	41.0
20	36.9957687333344	39.0	35.0	40.0	32.0	41.0
21	36.937441121525175	39.0	35.0	40.0	32.0	41.0
22	36.972073640007025	39.0	35.0	40.0	32.0	41.0
23	36.83764709639304	38.0	35.0	40.0	32.0	41.0
24	36.755177314023854	38.0	35.0	40.0	31.0	41.0
25	36.57708090501205	38.0	35.0	40.0	31.0	41.0
26	36.422743457503714	38.0	35.0	40.0	31.0	41.0
27	36.400485398138244	38.0	35.0	40.0	31.0	41.0
28	36.35572977374699	38.0	35.0	40.0	31.0	41.0
29	36.22195787893787	38.0	35.0	40.0	30.0	41.0
30	36.053808938351246	38.0	35.0	40.0	30.0	41.0
31	35.89792268757285	38.0	35.0	40.0	30.0	41.0
32	35.695779910265216	38.0	34.0	40.0	29.0	41.0
33	35.48429641220521	38.0	34.0	40.0	27.0	41.0
34	35.22981366459627	38.0	34.0	40.0	26.0	41.0
35	35.11892254386945	38.0	34.0	40.0	25.0	41.0
36	34.8842868319788	38.0	34.0	40.0	24.0	41.0
37	34.84540707978732	38.0	34.0	40.0	24.0	41.0
38	34.72391384183046	38.0	34.0	40.0	24.0	41.0
39	34.64110875153683	38.0	34.0	40.0	23.0	41.0
40	34.538456625524915	38.0	34.0	40.0	23.0	41.0
41	34.457663382777945	38.0	33.0	40.0	23.0	41.0
42	34.40866052467707	38.0	33.0	40.0	23.0	41.0
43	34.383720001916046	38.0	33.0	40.0	22.0	41.0
44	34.17723418863466	38.0	33.0	40.0	22.0	41.0
45	34.14648166184994	38.0	33.0	40.0	22.0	41.0
46	34.08475306966421	37.0	33.0	40.0	21.0	41.0
47	33.85859585814878	37.0	33.0	40.0	20.0	41.0
48	33.72765013013141	37.0	33.0	40.0	20.0	41.0
49	33.69247473215284	37.0	33.0	40.0	20.0	41.0
50	33.47830877069728	37.0	32.0	40.0	19.0	41.0
51	31.528700761628	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	1.0
13	1.0
14	4.0
15	4.0
16	8.0
17	16.0
18	39.0
19	69.0
20	140.0
21	229.0
22	375.0
23	487.0
24	720.0
25	889.0
26	1065.0
27	1151.0
28	1262.0
29	1398.0
30	1672.0
31	2063.0
32	2834.0
33	3792.0
34	5113.0
35	6406.0
36	6743.0
37	9097.0
38	9902.0
39	7142.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.05178112376056	23.584920723626436	27.789043414392694	15.574254738220313
2	31.303389803445686	28.37663063437066	27.889635791725876	12.430343770457775
3	27.557521276086156	28.03812930112248	31.271455715403405	13.132893707387952
4	24.29385747816507	28.208976672148683	32.812275463443456	14.684890386242794
5	22.157466988136488	32.48495106101007	30.024429577352347	15.333152373501093
6	19.668204825240704	42.58410640438136	27.844928068466686	9.902760701911255
7	72.5622315540724	14.213862587619156	10.64842165769851	2.575484200609941
8	78.63449839531208	7.067013683756726	10.645228248894282	3.653259672036916
9	71.85648820833799	8.60304331859043	13.086589279726644	6.453879193344935
10	29.657187564866117	38.686550958820995	18.912963643040765	12.743297833272127
11	27.873668747704738	26.70488112535726	29.623656772421718	15.79779335451628
12	26.313688546839327	22.8871608999026	33.36473518657491	17.434415366683165
13	19.684171869261842	34.58621405419215	31.847866004566576	13.881748071979436
14	16.765396222197385	33.18271088473391	33.39347586581296	16.658417027255744
15	15.278864423829216	26.59151511280717	42.028453272445674	16.101167190917945
16	16.8468281467052	26.848584521547526	37.07068610388159	19.233901227865687
17	16.47958613421897	24.9916173018889	33.54995289722014	24.978843666671988
18	18.593622762617954	25.1959954653595	36.778489198294714	19.431892573727826
19	21.18986412045538	27.904006131344904	32.86816011751744	18.03796963068227
20	22.310750610739433	26.81984384230947	32.2151080170528	18.65429752989829
21	19.406345303294	29.40171486052787	33.52759903559054	17.66434080058759
22	17.49189672515927	25.015567867920613	31.51734819332897	25.97518721359115
23	15.923933002283288	29.872742659151513	32.532852193073495	21.670472145491704
24	17.557361605645948	26.34721933928372	35.114723211291896	20.98069584377844
25	16.35184978204985	30.225614332018715	33.01026680930559	20.412269076625844
26	15.547110763384374	30.490667262769644	32.50570821823756	21.456513755608427
27	17.164572322725892	30.211243992399687	31.911734180651134	20.712449504223283
28	14.506059493206022	28.4724328984975	37.39960721071708	19.621900397579395
29	16.858005077519998	27.156748471155534	33.703236519823086	22.28200993150138
30	18.99439556754858	27.896022609334338	33.39028245700874	19.719299366108352
31	21.98981302591451	28.23771735138674	29.218093854284753	20.554375768413994
32	21.159526736815213	28.42453176643408	30.945728017372144	19.47021347937856
33	19.977965479250827	26.709671238563605	29.773746986220438	23.538616295965127
34	19.029523064395086	26.990691213335676	31.9644254259209	22.015360296348337
35	19.094987944881765	26.069392773315876	31.68819556435517	23.14742371744719
36	21.279279566973766	26.49890625748455	32.34444107362404	19.87737310191764
37	20.126458988647432	26.851777930351755	33.936355362531735	19.08540771846908
38	17.247600951635825	27.805010458413832	31.578022960609303	23.36936562934104
39	20.32445033450957	24.77446550320139	32.515288444650245	22.385795717638793
40	20.972712321767872	24.435964169953216	31.071867665139152	23.51945584313976
41	18.600009580226413	25.015567867920613	31.570039438598734	24.814383113254245
42	20.064187516964985	25.1736416037299	31.881396797010968	22.880774082294145
43	20.610260422487986	23.370962333743154	33.82618275878587	22.192594484982994
44	18.280668699803606	25.71013428284022	32.87933704833224	23.129859969023936
45	17.99006849861885	27.097670408277313	31.30977662105414	23.60248447204969
46	21.381468648709063	26.688914081336122	30.434782608695656	21.49483466125916
47	18.45630618403615	25.210365804978522	34.21897204170592	22.114355969279405
48	18.762873429242045	25.009181050312158	33.48289131233135	22.74505420811445
49	18.80917785690335	22.95422248479139	35.43885420492104	22.797745453384216
50	18.312602787845886	24.52058950326526	33.75273435628862	23.414073352600234
51	17.785690335148256	24.083092497086014	30.838748822430507	27.29246834533523
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	130.0
1	148.0
2	166.0
3	539.5
4	913.0
5	773.5
6	634.0
7	502.5
8	371.0
9	396.5
10	422.0
11	411.5
12	401.0
13	407.5
14	414.0
15	407.0
16	400.0
17	395.0
18	390.0
19	415.0
20	440.0
21	434.5
22	429.0
23	477.0
24	525.0
25	617.5
26	779.5
27	849.0
28	949.0
29	1049.0
30	1242.5
31	1436.0
32	1584.0
33	1732.0
34	1881.5
35	2031.0
36	2121.0
37	2211.0
38	2495.5
39	2780.0
40	3422.5
41	4065.0
42	4449.5
43	4834.0
44	5322.0
45	5810.0
46	6814.0
47	7818.0
48	7152.0
49	6486.0
50	5963.0
51	5440.0
52	4521.5
53	3603.0
54	2934.0
55	2265.0
56	1871.5
57	1478.0
58	1191.0
59	904.0
60	724.5
61	545.0
62	437.5
63	330.0
64	282.0
65	234.0
66	186.5
67	139.0
68	103.0
69	67.0
70	63.0
71	59.0
72	59.5
73	60.0
74	41.5
75	19.5
76	16.0
77	12.5
78	9.0
79	6.5
80	4.0
81	4.0
82	4.0
83	2.5
84	1.0
85	1.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	62629.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.96485653610947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.40414891646601	56.82990308004279
2	8.686793850713096	11.98166983346373
3	3.7784774958325613	7.817464752750324
4	2.1971661418781254	6.061089910424883
5	1.1043711798481202	3.8081399990419778
6	0.7709761066864235	3.1902153954238455
7	0.3935914058158918	1.9000782385157036
8	0.2315243563622893	1.2773635216912294
9	0.15512131876273386	0.9628127544747641
>10	0.24541581774402665	2.639352376694503
>50	0.018521948508983144	0.9388621884430536
>100	0.013891461381737358	2.5930479490331955
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	467	0.7456609557872551	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCG	319	0.5093487042743777	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGC	294	0.4694310942215268	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTC	244	0.38959587411582497	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	184	0.29379360998898274	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	116	0.18521771064522824	No Hit
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT	97	0.15488032700506157	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	89	0.14210669178814925	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	77	0.12294623896278083	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	70	0.11176930814798255	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT	68	0.1085758993437545	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	66	0.10538249053952643	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTC	65	0.10378578613741238	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.24269906912133357	0.0
2	0.0	0.0	0.0	0.7712082262210797	0.0
3	0.0	0.0	0.0	1.0123105909402992	0.0
4	0.0	0.0	0.0	1.393922943045554	0.0
5	0.0	0.0	0.0	2.6553194207156428	0.0
6	0.0	0.0	0.0	3.361062766450047	0.0
7	0.0	0.0	0.0	3.9326829424068723	0.0
8	0.0	0.0	0.0	5.289881684203803	0.0
9	0.0	0.0	0.0	5.920579923038848	0.0
10	0.0	0.0	0.0	7.819061457152437	0.0
11	0.0	0.0	0.0	9.299206437912149	0.0
12	0.0	0.0	0.0	11.124239569528493	0.0
13	0.0	0.0	0.0	11.68468281467052	0.0
14	0.0	0.0	0.0	11.919398361781283	0.0
15	0.0	0.0	0.0	12.390426160404925	0.0
16	0.0	0.0	0.0	13.239872902329592	0.0
17	0.0	0.0	0.0	14.15318782033882	0.0
18	0.0	0.0	0.0	15.650896549521788	0.0
19	0.0	0.0	0.0	16.246467291510324	0.0
20	0.0	0.0	0.0	16.9601941592553	0.0
21	0.0	0.0	0.0	17.68509795781507	0.0
22	0.0	0.0	0.0	18.376470963930448	0.0
23	0.0	0.0	0.0	19.17322646058535	0.0
24	0.0	0.0	0.0	19.76241038496543	0.0
25	0.0	0.0	0.0	20.214277730763705	0.0
26	0.0	0.0	0.0	20.69488575580003	0.0
27	0.0	0.0	0.0	21.17868718964058	0.0
28	0.0	0.0	0.0	21.691229302719186	0.0
29	0.0	0.0	0.0	22.213351642210476	0.0
30	0.0	0.0	0.0	22.840856472241295	0.0
31	0.0	0.0	0.0	23.44760414504463	0.0
32	0.0	0.0	0.0	23.968129780133804	0.0
33	0.0	0.0	0.0	24.59882801896885	0.0
34	0.0	0.0	0.0	25.14170751568762	0.0
35	0.0	0.0	0.0	25.73887496207827	0.0
36	0.0	0.0	0.0	26.240240144342078	0.0
37	0.0	0.0	0.0	26.797489980679877	0.0
38	0.0	0.0	0.0	27.41860799310224	0.0
39	0.0	0.0	0.0	27.991824873461177	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGGG	30	2.1326687E-6	45.000004	3
ACTAATC	30	2.1326687E-6	45.000004	10
AAGTAGG	20	6.9822365E-4	45.000004	2
TGCACGG	20	6.9822365E-4	45.000004	2
GGCACCG	20	6.9822365E-4	45.000004	8
TACAGGG	30	2.1326687E-6	45.000004	3
GCGACTG	20	6.9822365E-4	45.000004	11
GATATGG	20	6.9822365E-4	45.000004	2
TCCTAGC	30	2.1326687E-6	45.000004	15
GAAGTTG	20	6.9822365E-4	45.000004	9
GACGGGA	30	2.1326687E-6	45.000004	4
GGCGATG	40	6.6393113E-9	45.000004	8
GGCGACT	20	6.9822365E-4	45.000004	10
AAGGGTG	20	6.9822365E-4	45.000004	5
AAGGGCG	20	6.9822365E-4	45.000004	5
TCACGGG	20	6.9822365E-4	45.000004	3
TCTAAGG	20	6.9822365E-4	45.000004	2
GCACGGG	20	6.9822365E-4	45.000004	3
CGGGATG	20	6.9822365E-4	45.000004	6
AGTAAAG	20	6.9822365E-4	45.000004	1
>>END_MODULE