SRR3549496_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549496_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	156532
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1609	1.0279048373495514	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG	1318	0.842000357754325	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC	1056	0.6746224414177293	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC	898	0.5736846140086372	RNA PCR Primer, Index 30 (95% over 21bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	603	0.38522474637773746	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	354	0.2261518411570797	No Hit
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	314	0.20059796080034753	Illumina Single End Adapter 2 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCT	281	0.1795160095060435	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	263	0.16801676334551402	No Hit
GAACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT	202	0.12904709580149745	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	195	0.12457516673906933	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTC	191	0.12201977870339611	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTA	179	0.11435361459637647	No Hit
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	170	0.10860399151611172	RNA PCR Primer, Index 30 (96% over 25bp)
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	165	0.1054097564715202	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTTA	75	0.0	45.000004	1
AGCGGGA	75	0.0	45.000004	4
TATACTA	65	0.0	45.000004	44
GTTTGCG	25	3.8753256E-5	45.0	1
AACGTAG	20	7.014603E-4	45.0	18
AGGTAAG	20	7.014603E-4	45.0	1
AGATCAC	20	7.014603E-4	45.0	38
ATAGGGC	50	2.1827873E-11	45.0	4
GGCACCG	45	3.8016879E-10	45.0	8
TTGCTAG	20	7.014603E-4	45.0	1
GTAGGGC	35	1.2029523E-7	45.0	4
TGCGGGC	25	3.8753256E-5	45.0	4
AGGCGAT	35	1.2029523E-7	45.0	7
CAGGGCG	35	1.2029523E-7	45.0	5
AAACGAT	25	3.8753256E-5	45.0	43
GTAGCAG	40	6.7502697E-9	45.0	1
AGCATGG	25	3.8753256E-5	45.0	2
CGTAGGG	20	7.014603E-4	45.0	3
CGGGCGT	20	7.014603E-4	45.0	6
CGGGCGA	20	7.014603E-4	45.0	6