##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549496_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	156532
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.451243196279353	31.0	31.0	34.0	30.0	34.0
2	31.539525464441777	31.0	31.0	34.0	30.0	34.0
3	31.02172079830322	31.0	31.0	34.0	27.0	34.0
4	35.21528505353538	37.0	35.0	37.0	33.0	37.0
5	35.12453044744844	35.0	35.0	37.0	33.0	37.0
6	35.1800718064038	36.0	35.0	37.0	32.0	37.0
7	32.03601180589273	36.0	35.0	37.0	11.0	37.0
8	33.553554544757624	37.0	35.0	37.0	19.0	37.0
9	36.55787315053791	39.0	35.0	39.0	32.0	39.0
10	36.41485447065137	38.0	35.0	39.0	32.0	39.0
11	36.47042777195717	38.0	35.0	39.0	32.0	39.0
12	36.2929944037002	38.0	35.0	39.0	32.0	39.0
13	32.442433496026375	37.0	33.0	39.0	10.0	39.0
14	34.89400250428027	38.0	33.0	40.0	18.0	41.0
15	36.28300922495081	38.0	34.0	40.0	30.0	41.0
16	36.9657450233818	38.0	35.0	40.0	32.0	41.0
17	37.106751335190246	38.0	35.0	40.0	32.0	41.0
18	37.00240206475353	39.0	35.0	40.0	32.0	41.0
19	36.95172872000613	38.0	35.0	40.0	32.0	41.0
20	36.868327242991846	38.0	35.0	40.0	32.0	41.0
21	36.83744537858074	38.0	35.0	40.0	32.0	41.0
22	36.86557381238341	38.0	35.0	40.0	32.0	41.0
23	36.756522627961054	38.0	35.0	40.0	32.0	41.0
24	36.633819282958115	38.0	35.0	40.0	31.0	41.0
25	36.44245904990673	38.0	35.0	40.0	31.0	41.0
26	36.300864998850074	38.0	35.0	40.0	31.0	41.0
27	36.284178314977126	38.0	35.0	40.0	31.0	41.0
28	36.2265543147727	38.0	35.0	40.0	31.0	41.0
29	36.17516546137531	38.0	35.0	40.0	31.0	41.0
30	36.035379347353896	38.0	35.0	40.0	30.0	41.0
31	35.836122965272274	38.0	35.0	40.0	30.0	41.0
32	35.58659571205887	38.0	34.0	40.0	29.0	41.0
33	35.31966626632254	38.0	34.0	40.0	27.0	41.0
34	34.96598778524519	38.0	34.0	40.0	25.0	41.0
35	34.80612909820356	38.0	34.0	40.0	25.0	41.0
36	34.555848005519636	38.0	34.0	40.0	23.0	41.0
37	34.39911966882171	38.0	33.0	40.0	23.0	41.0
38	34.29472567909437	37.0	33.0	40.0	23.0	41.0
39	34.048430991746095	37.0	33.0	40.0	21.0	41.0
40	33.906166151330076	37.0	33.0	40.0	21.0	41.0
41	33.81758362507347	37.0	33.0	40.0	20.0	41.0
42	33.84376996396903	37.0	33.0	40.0	20.0	41.0
43	33.84117624511282	37.0	33.0	40.0	20.0	41.0
44	33.639843610252214	37.0	33.0	40.0	18.0	41.0
45	33.56027521529144	37.0	33.0	40.0	18.0	41.0
46	33.37648531929573	37.0	33.0	40.0	18.0	41.0
47	33.09858687041627	36.0	32.0	40.0	17.0	41.0
48	33.01621393708635	36.0	32.0	40.0	17.0	41.0
49	32.99838371706743	36.0	32.0	40.0	15.0	41.0
50	32.81280504944676	36.0	31.0	40.0	15.0	41.0
51	30.89183681292004	35.0	28.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	2.0
12	0.0
13	1.0
14	5.0
15	4.0
16	18.0
17	29.0
18	91.0
19	204.0
20	315.0
21	574.0
22	866.0
23	1260.0
24	1790.0
25	2594.0
26	3191.0
27	3408.0
28	3549.0
29	3770.0
30	4554.0
31	5652.0
32	7283.0
33	10185.0
34	14078.0
35	16910.0
36	15745.0
37	20727.0
38	22229.0
39	17488.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.82758796923313	22.233792451383742	26.318580226407377	17.62003935297575
2	31.279227250658014	29.006848439935606	27.803899522142437	11.910024787263946
3	27.936779699997444	29.221501034932157	30.95916489918994	11.882554365880459
4	23.13839981601206	26.19847698873074	36.950272148825796	13.712851046431402
5	22.760202386732427	31.124626274499782	31.416579357575447	14.698591981192344
6	19.240794214601486	41.06444688625968	29.837988398538318	9.856770500600517
7	70.06107377405259	14.084660005621854	13.288656632509646	2.56560958781591
8	76.21317046993586	7.5275343060843785	12.687501597117523	3.5717936268622386
9	70.55618020596428	7.522423530013032	14.117241203076686	7.804155060946005
10	33.43150282370378	34.47985076533872	19.970996345795108	12.117650065162396
11	31.920629647611992	24.444841949249994	28.956379526231057	14.678148876906958
12	29.357575447831753	21.658830143357267	32.13017146653719	16.853422942273784
13	18.679886540771214	37.11381698310888	31.496435233690235	12.709861242429662
14	16.03697646487619	34.690031431272836	32.909564817417525	16.363427286433446
15	13.388316765900901	24.994889223928656	47.72570464825084	13.891089361919606
16	15.289525464441775	24.869675210180667	40.099149055784125	19.74165026959344
17	14.940076150563463	21.896481230674876	35.259882963227966	27.903559655533694
18	17.705006005161884	23.933125495106434	39.351059208340786	19.0108092913909
19	21.203332225998516	25.731479825211455	33.27626300053663	19.788924948253392
20	23.13137249891396	24.692714588710295	34.1163468172642	18.059566095111542
21	18.11770117292311	28.960212608284568	34.64403444663072	18.278051772161604
22	17.2169268903483	23.821327268545726	32.48345386246901	26.47829197863696
23	14.724784708557994	30.124191858533717	33.11782894232489	22.033194490583394
24	17.022717399637134	24.594332149336875	37.84657450233818	20.53637594868781
25	14.848721028288145	29.4534024991695	36.38744793396877	19.310428538573582
26	15.330411673012549	30.135691104694246	33.05777732348657	21.476119898806633
27	15.723302583497304	31.322668847264456	33.05777732348657	19.896251245751667
28	13.34806940433905	28.332225998517874	39.41366621521478	18.906038381928294
29	16.27079447014029	26.92037410880842	35.22091329568395	21.587918125367338
30	16.958193851736386	29.126951677612244	35.949837732859734	17.965016737791633
31	21.82620805969386	28.422942273784273	29.0694554468096	20.681394219712264
32	23.991899419926916	27.696573224644162	30.817979710218996	17.493547645209926
33	21.86645542125572	26.267472465693913	29.66102777706795	22.205044335982418
34	18.06020494212046	29.488539084660005	30.581606316919224	21.869649656300307
35	17.804666138553138	27.168246748268725	33.06991541665602	21.957171696522117
36	22.896276799632023	27.14013747987632	29.72746786599545	20.236117854496204
37	19.335343571921396	29.21830679988756	33.055221935450895	18.39112769274014
38	20.28786446221859	29.79135256688728	27.27621189277592	22.64457107811821
39	21.57386349117113	28.62865101065597	29.053484271586637	20.744001226586256
40	22.597935246467177	26.682723021490812	30.191909641479057	20.527432090562954
41	16.22990826156952	27.217437967955433	29.623335803541767	26.92931796693328
42	18.59619758260292	26.777272378810725	31.513684102931027	23.11284593565533
43	20.27700406306698	24.213579332021567	30.86269900084328	24.646717604068176
44	18.903482993892624	26.594562134260087	31.044770398384998	23.457184473462295
45	17.817443078731504	29.8827076891626	29.017708839087213	23.28214039301868
46	22.433112718166253	30.0258094191603	28.102879922316205	19.438197940357245
47	17.582986226458488	27.81859300334756	33.11910663634273	21.479314133851226
48	19.266348094958218	26.402269184575676	32.109089515242886	22.222293205223213
49	19.392839802724044	23.52681879743439	34.8631589706897	22.217182429151865
50	19.11110827179107	27.349040451792604	31.540515677305596	21.999335599110726
51	17.88771624971252	26.866072113050365	29.35502005979608	25.891191577441035
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	287.0
1	329.5
2	372.0
3	1610.0
4	2848.0
5	2426.5
6	2005.0
7	1593.5
8	1182.0
9	1190.0
10	1198.0
11	1182.0
12	1166.0
13	1126.5
14	1087.0
15	1056.0
16	1025.0
17	988.0
18	951.0
19	969.0
20	987.0
21	1003.5
22	1020.0
23	1093.0
24	1166.0
25	1331.5
26	1670.0
27	1843.0
28	2165.0
29	2487.0
30	2943.5
31	3400.0
32	3896.0
33	4392.0
34	4767.0
35	5142.0
36	5438.5
37	5735.0
38	6592.5
39	7450.0
40	8695.0
41	9940.0
42	11629.0
43	13318.0
44	14349.0
45	15380.0
46	17813.0
47	20246.0
48	17818.0
49	15390.0
50	13905.5
51	12421.0
52	10371.5
53	8322.0
54	6851.0
55	5380.0
56	4260.0
57	3140.0
58	2546.0
59	1952.0
60	1653.5
61	1355.0
62	1118.0
63	881.0
64	740.5
65	600.0
66	473.0
67	346.0
68	284.0
69	222.0
70	185.5
71	149.0
72	130.5
73	112.0
74	91.5
75	54.0
76	37.0
77	26.5
78	16.0
79	10.5
80	5.0
81	6.0
82	7.0
83	4.5
84	2.0
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	156532.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.820483990493955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.62043982817208	39.66728847775535
2	8.220811694556646	8.191296348350496
3	3.2852471629159457	4.910178110546086
4	1.7259729435147784	3.43955229601615
5	1.1425274091171378	2.8460634247310455
6	0.9424889401808041	2.8173153093297216
7	0.6783355773546195	2.3656504740244806
8	0.5911393216644226	2.356067768890706
9	0.5385651086747452	2.4148416937111903
>10	3.2031800987369365	24.71635192804027
>50	0.025645957555940246	0.9148289167710117
>100	0.019234468166955183	1.8571282549255104
>500	0.0025645957555940247	0.9589093603863748
>1k	0.0038468936333910368	2.5445276365216056
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1609	1.0279048373495514	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG	1318	0.842000357754325	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC	1056	0.6746224414177293	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC	898	0.5736846140086372	RNA PCR Primer, Index 30 (95% over 21bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	603	0.38522474637773746	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	354	0.2261518411570797	No Hit
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	314	0.20059796080034753	Illumina Single End Adapter 2 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCT	281	0.1795160095060435	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	263	0.16801676334551402	No Hit
GAACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT	202	0.12904709580149745	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	195	0.12457516673906933	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTC	191	0.12201977870339611	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTA	179	0.11435361459637647	No Hit
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	170	0.10860399151611172	RNA PCR Primer, Index 30 (96% over 25bp)
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	165	0.1054097564715202	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18845986763089975	0.0
2	0.0	0.0	0.0	0.7436179183809062	0.0
3	0.0	0.0	0.0	0.9633812894488027	0.0
4	0.0	0.0	0.0	1.4444330871642859	0.0
5	0.0	0.0	0.0	3.0076917179873766	0.0
6	0.0	0.0	0.0	3.6510106559681086	0.0
7	0.0	0.0	0.0	4.322438862341246	0.0
8	0.0	0.0	0.0	5.448087292055298	0.0
9	0.0	0.0	0.0	5.8575882247719315	0.0
10	0.0	0.0	0.0	7.93384100375642	0.0
11	0.0	0.0	0.0	8.906804998338998	0.0
12	0.0	0.0	0.0	10.788209439603405	0.0
13	0.0	0.0	0.0	11.308869751871821	0.0
14	0.0	0.0	0.0	11.549076227225104	0.0
15	0.0	0.0	0.0	12.03651649502977	0.0
16	0.0	0.0	0.0	12.72966549970613	0.0
17	0.0	0.0	0.0	13.455395701837324	0.0
18	0.0	0.0	0.0	14.714563156415302	0.0
19	0.0	0.0	0.0	15.132369100247873	0.0
20	0.0	0.0	0.0	15.665167505685739	0.0
21	0.0	0.0	0.0	16.20754861625738	0.0
22	0.0	0.0	0.0	16.744180103748754	0.0
23	0.0	0.0	0.0	17.295505072445252	0.0
24	0.0	0.0	0.0	17.6915902179746	0.0
25	0.0	0.0	0.0	18.074898423325582	0.0
26	0.0	0.0	0.0	18.42179234916822	0.0
27	0.0	0.0	0.0	18.778268980144635	0.0
28	0.0	0.0	0.0	19.147522551299414	0.0
29	6.388470089183043E-4	0.0	0.0	19.53274729767715	0.0
30	6.388470089183043E-4	0.0	0.0	20.04638029284747	0.0
31	6.388470089183043E-4	0.0	0.0	20.466102777706794	0.0
32	6.388470089183043E-4	0.0	0.0	20.832800960825903	0.0
33	6.388470089183043E-4	0.0	0.0	21.276799632024122	0.0
34	6.388470089183043E-4	0.0	0.0	21.68118978866941	0.0
35	6.388470089183043E-4	0.0	0.0	22.138604247054914	0.0
36	6.388470089183043E-4	0.0	0.0	22.552577108833976	0.0
37	6.388470089183043E-4	0.0	0.0	22.994020391996525	0.0
38	6.388470089183043E-4	0.0	0.0	23.381161679401018	0.0
39	6.388470089183043E-4	0.0	0.0	23.837298443768685	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTTA	75	0.0	45.000004	1
AGCGGGA	75	0.0	45.000004	4
TATACTA	65	0.0	45.000004	44
GTTTGCG	25	3.8753256E-5	45.0	1
AACGTAG	20	7.014603E-4	45.0	18
AGGTAAG	20	7.014603E-4	45.0	1
AGATCAC	20	7.014603E-4	45.0	38
ATAGGGC	50	2.1827873E-11	45.0	4
GGCACCG	45	3.8016879E-10	45.0	8
TTGCTAG	20	7.014603E-4	45.0	1
GTAGGGC	35	1.2029523E-7	45.0	4
TGCGGGC	25	3.8753256E-5	45.0	4
AGGCGAT	35	1.2029523E-7	45.0	7
CAGGGCG	35	1.2029523E-7	45.0	5
AAACGAT	25	3.8753256E-5	45.0	43
GTAGCAG	40	6.7502697E-9	45.0	1
AGCATGG	25	3.8753256E-5	45.0	2
CGTAGGG	20	7.014603E-4	45.0	3
CGGGCGT	20	7.014603E-4	45.0	6
CGGGCGA	20	7.014603E-4	45.0	6
>>END_MODULE