SRR3549495_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549495_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	518645
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3131	0.6036884574227073	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	1450	0.2795746608952173	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1319	0.2543165363591667	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	1229	0.236963626372567	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	1081	0.20842772995015857	No Hit
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	935	0.18027745374967463	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	799	0.15405527865881288	TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	693	0.13361740689681767	TruSeq Adapter, Index 13 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	20	7.029703E-4	45.0	28
CCTAGCG	20	7.029703E-4	45.0	1
CGGCACC	20	7.029703E-4	45.0	14
GACCGCG	25	3.8878352E-5	45.0	20
CGACAAG	20	7.029703E-4	45.0	27
ACGGGTC	20	7.029703E-4	45.0	5
AACGGTA	20	7.029703E-4	45.0	19
TGACCGC	25	3.8878352E-5	45.0	19
CGGGCAA	45	3.8380676E-10	45.0	6
GTCGTTT	35	1.21021E-7	45.0	9
AATCTCG	20	7.029703E-4	45.0	18
GTCGTGG	25	3.8878352E-5	45.0	2
ATGACCG	25	3.8878352E-5	45.0	18
TCCCGGT	20	7.029703E-4	45.0	20
CGGTCTA	20	7.029703E-4	45.0	31
AGCTACG	35	1.21021E-7	45.0	9
CCCGGTA	20	7.029703E-4	45.0	21
CGGTTTT	500	0.0	43.65	1
CGGGAAC	100	0.0	42.75	6
TACGGGA	230	0.0	42.06522	4