##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549495_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	518645
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.365849473146373	31.0	31.0	34.0	30.0	34.0
2	31.50553847043739	31.0	31.0	34.0	30.0	34.0
3	31.16897878124729	31.0	31.0	34.0	28.0	34.0
4	35.22175283671876	37.0	35.0	37.0	33.0	37.0
5	35.04313354992336	35.0	35.0	37.0	32.0	37.0
6	35.074382284607005	36.0	35.0	37.0	32.0	37.0
7	32.12668395530662	35.0	35.0	37.0	17.0	37.0
8	33.60574574130668	37.0	35.0	37.0	19.0	37.0
9	36.443440117999785	39.0	35.0	39.0	32.0	39.0
10	36.2124439645615	37.0	35.0	39.0	32.0	39.0
11	36.319326321472296	38.0	35.0	39.0	32.0	39.0
12	36.37528559997686	38.0	35.0	39.0	32.0	39.0
13	32.72574111386401	37.0	34.0	39.0	10.0	39.0
14	35.210369327767545	38.0	34.0	40.0	18.0	41.0
15	36.51539299520867	38.0	34.0	40.0	30.0	41.0
16	37.11329329310029	38.0	36.0	40.0	32.0	41.0
17	37.22329917380868	39.0	36.0	40.0	32.0	41.0
18	37.17186514860839	39.0	36.0	40.0	32.0	41.0
19	37.187249467362065	39.0	36.0	40.0	32.0	41.0
20	37.139147200879215	39.0	35.0	40.0	32.0	41.0
21	37.05644515998419	39.0	35.0	40.0	32.0	41.0
22	37.059703650859454	39.0	35.0	40.0	32.0	41.0
23	36.944752190804884	39.0	35.0	40.0	31.0	41.0
24	36.858413751217114	39.0	35.0	40.0	31.0	41.0
25	36.67099653905851	38.0	35.0	40.0	31.0	41.0
26	36.550787147278	38.0	35.0	40.0	31.0	41.0
27	36.53774354327141	38.0	35.0	40.0	31.0	41.0
28	36.36324075234505	38.0	35.0	40.0	30.0	41.0
29	36.34145513790743	38.0	35.0	40.0	30.0	41.0
30	36.175975860174105	38.0	35.0	40.0	30.0	41.0
31	36.071312747640484	38.0	35.0	40.0	30.0	41.0
32	35.940915269596736	38.0	35.0	40.0	29.0	41.0
33	35.76234996963241	38.0	35.0	40.0	29.0	41.0
34	35.623411003673034	38.0	35.0	40.0	27.0	41.0
35	35.44726354250017	38.0	34.0	40.0	27.0	41.0
36	35.32068177655236	38.0	34.0	40.0	26.0	41.0
37	35.220040682933416	38.0	34.0	40.0	25.0	41.0
38	35.10066230273115	38.0	34.0	40.0	25.0	41.0
39	34.968356004588884	38.0	34.0	40.0	24.0	41.0
40	34.9326919183642	38.0	34.0	40.0	24.0	41.0
41	34.85487954188318	38.0	34.0	40.0	24.0	41.0
42	34.81473840488195	38.0	34.0	40.0	24.0	41.0
43	34.74595532589729	38.0	34.0	40.0	24.0	41.0
44	34.52216255820455	38.0	33.0	40.0	23.0	41.0
45	34.51042620675028	38.0	33.0	40.0	23.0	41.0
46	34.381571209594235	38.0	33.0	40.0	23.0	41.0
47	34.18213999942157	38.0	33.0	40.0	22.0	41.0
48	34.05832891476829	37.0	33.0	40.0	22.0	41.0
49	33.95586190939853	37.0	33.0	40.0	21.0	41.0
50	33.72957996317327	37.0	32.0	40.0	20.0	41.0
51	31.703110991140374	35.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	11.0
10	9.0
11	9.0
12	10.0
13	4.0
14	8.0
15	21.0
16	41.0
17	112.0
18	262.0
19	514.0
20	913.0
21	1482.0
22	2284.0
23	3437.0
24	4857.0
25	6617.0
26	8203.0
27	9316.0
28	10519.0
29	11843.0
30	14486.0
31	17852.0
32	23101.0
33	30026.0
34	40437.0
35	49109.0
36	58104.0
37	77042.0
38	85418.0
39	62588.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.53325492388821	23.659535906062914	26.512354307859905	15.294854862188972
2	31.59463602271303	26.895275188230872	28.052135854004185	13.457952935051914
3	29.46485553702436	26.415756442267835	31.189349169470447	12.930038851237358
4	26.182070587781624	27.796084026646355	31.72844623972081	14.293399145851208
5	23.12660875936334	32.33117064658871	29.301159752817433	15.241060841230514
6	20.477397834742455	41.94718931060745	27.190081847892106	10.385331006757994
7	74.52322879811817	13.225038321009555	9.59847294392118	2.6532599369510934
8	79.09321404814469	5.643551947864147	10.244965245977498	5.01826875801367
9	71.36519199066798	8.358318310212187	13.840102574979033	6.43638712414079
10	27.377107655525457	40.32565627741519	18.871482420538133	13.425753646521224
11	25.96747293428067	26.760886540890205	31.401054671306966	15.87058585352216
12	24.991660962700884	23.080719952954333	33.20267234813794	18.724946736206846
13	20.08869265104262	32.86274812251154	32.00011568606658	15.048443540379258
14	17.158364584638818	31.586923618274543	32.22069045300736	19.03402134407928
15	16.253313923782166	27.087892489082126	40.472191961746475	16.186601625389237
16	17.637497710379932	26.787108715981066	35.86518717041522	19.710206403223786
17	17.402269375006025	26.103982492841926	32.775983572578546	23.717764559573503
18	18.509577842262047	25.71662697991883	36.08846127890947	19.68533389890966
19	21.371843939496188	28.388203877411332	31.19359099191162	19.046361191180868
20	22.445603447444785	26.7063212794879	31.249120303868732	19.598954969198584
21	20.682547792806254	29.179110952578352	31.584609896942993	18.553731357672394
22	18.323323275072546	26.221982280750805	30.384174146092217	25.070520298084432
23	17.220835060590577	29.415496148618033	31.11627413741577	22.247394653375625
24	17.645210114818422	26.276161921931184	34.344108205034274	21.73451975821612
25	17.447965371304072	29.54622140385042	30.69941867751545	22.30639454733006
26	16.453258008849982	28.53898138418379	32.60457538393313	22.403185223033095
27	19.37895863259069	27.563940653047847	30.887601345814574	22.169499368546887
28	16.842734432993666	28.410569850282947	33.94942590789461	20.797269808828776
29	18.53753530835157	26.325328500226554	30.930212380337224	24.206923811084653
30	19.391298479692274	26.776311349767184	31.931860906785953	21.900529263754592
31	20.664038022153882	27.54716617339413	28.76534045445343	23.023455349998553
32	20.79244955605472	27.891332221461695	29.444417665262367	21.87180055722122
33	18.964031273799996	26.69186052116573	30.77943487356477	23.564673331469503
34	19.38840632802784	25.66321857918229	30.216043729333165	24.7323313634567
35	17.518148251694317	26.21696921786579	32.00512874895159	24.259753781488303
36	19.997686278668453	26.573475113034927	31.214221673784575	22.214616934512048
37	17.857879667209748	28.377984941530332	32.06316459235122	21.700970798908696
38	18.148637314540775	27.95997262096424	31.159463602271302	22.73192646222368
39	19.235700720145765	25.811682364623202	31.032208929036237	23.920407986194796
40	18.58515940575924	24.596978665561224	34.323477523161316	22.494384405518225
41	16.722035303531317	24.769736524983372	32.936980015231995	25.57124815625331
42	19.512190419265586	25.53615671605819	32.75901628281387	22.192636581862352
43	19.48037675095682	24.812154749395056	33.872301863509726	21.835166636138396
44	19.389563188693614	26.78633747553722	30.194063376683474	23.630035959085692
45	17.480357469945723	26.714226494037348	30.980535819298368	24.824880216718565
46	20.488966441400187	26.68491935717109	30.240144993203444	22.58596920822528
47	17.89432077818161	25.046419034214157	33.23332915578093	23.82593103182331
48	17.94888603958392	25.085173866517565	32.444928612056415	24.52101148184211
49	19.436416045657435	23.7106305854679	33.348629602136334	23.504323766738327
50	18.087709319476712	23.729140356120276	33.819664703217036	24.363485621185976
51	17.82934377078734	23.00745211078869	31.098728417318206	28.064475701105767
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	455.0
1	614.0
2	773.0
3	3387.0
4	6001.0
5	5330.5
6	4660.0
7	3674.0
8	2688.0
9	2652.5
10	2617.0
11	2604.5
12	2592.0
13	2546.0
14	2500.0
15	2435.5
16	2371.0
17	2305.0
18	2239.0
19	2393.0
20	2547.0
21	2686.0
22	2825.0
23	3174.5
24	3524.0
25	4323.5
26	6017.5
27	6912.0
28	8253.0
29	9594.0
30	11177.0
31	12760.0
32	14137.0
33	15514.0
34	16473.0
35	17432.0
36	18985.0
37	20538.0
38	22767.5
39	24997.0
40	27495.0
41	29993.0
42	33587.0
43	37181.0
44	41115.0
45	45049.0
46	52723.5
47	60398.0
48	59076.5
49	57755.0
50	53034.5
51	48314.0
52	40484.5
53	32655.0
54	26605.0
55	20555.0
56	17090.0
57	13625.0
58	11440.5
59	9256.0
60	7672.5
61	6089.0
62	4830.0
63	3571.0
64	2808.0
65	2045.0
66	1633.0
67	1221.0
68	1014.0
69	807.0
70	691.5
71	576.0
72	480.5
73	385.0
74	304.0
75	179.5
76	136.0
77	107.5
78	79.0
79	59.0
80	39.0
81	28.5
82	18.0
83	11.5
84	5.0
85	4.0
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	518645.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.54126401102859
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.52649992278366	40.19369828470454
2	9.03383340436613	9.131627182434354
3	3.466112544862214	5.25545127665458
4	1.800561063261721	3.6401052826515596
5	1.0319829532413034	2.6078861447324844
6	0.7278421973111682	2.2071638791602544
7	0.4983518379325639	1.7631132267931726
8	0.44452720033304255	1.7973573273692558
9	0.35666052525193553	1.622346639216318
>10	3.0597278138213437	26.98214751748657
>50	0.03494241701658153	1.2020303230334028
>100	0.015984297101718805	1.5986364483563948
>500	0.001115183518724568	0.4559749277425044
>1k	0.00185863919787428	1.5424615396645904
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3131	0.6036884574227073	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTC	1450	0.2795746608952173	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1319	0.2543165363591667	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCG	1229	0.236963626372567	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGC	1081	0.20842772995015857	No Hit
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	935	0.18027745374967463	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	799	0.15405527865881288	TruSeq Adapter, Index 16 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	693	0.13361740689681767	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9281011096221887E-4	0.0	0.0	0.24911066336318677	0.0
2	1.9281011096221887E-4	0.0	0.0	0.8570409432270628	0.0
3	1.9281011096221887E-4	0.0	0.0	1.1238901367987737	0.0
4	1.9281011096221887E-4	0.0	0.0	1.6215330331922606	0.0
5	1.9281011096221887E-4	0.0	0.0	2.9226156619653136	0.0
6	1.9281011096221887E-4	0.0	0.0	3.8502251058045482	0.0
7	1.9281011096221887E-4	0.0	0.0	4.569599629804587	0.0
8	1.9281011096221887E-4	0.0	0.0	6.7292656826924	0.0
9	1.9281011096221887E-4	0.0	0.0	7.604430776349912	0.0
10	1.9281011096221887E-4	0.0	0.0	9.523469810756875	0.0
11	1.9281011096221887E-4	0.0	0.0	10.893964079476328	0.0
12	1.9281011096221887E-4	0.0	0.0	12.438565878394662	0.0
13	1.9281011096221887E-4	0.0	0.0	13.033963501045994	0.0
14	1.9281011096221887E-4	0.0	0.0	13.298884593508083	0.0
15	1.9281011096221887E-4	0.0	0.0	13.70609954786029	0.0
16	1.9281011096221887E-4	0.0	0.0	14.501826875801367	0.0
17	1.9281011096221887E-4	0.0	0.0	15.349420123591281	0.0
18	3.8562022192443774E-4	0.0	0.0	16.594009389852403	0.0
19	3.8562022192443774E-4	0.0	0.0	17.12115223322311	0.0
20	3.8562022192443774E-4	0.0	0.0	17.78075562282486	0.0
21	3.8562022192443774E-4	0.0	0.0	18.459640023522834	0.0
22	3.8562022192443774E-4	0.0	0.0	19.188076622738098	0.0
23	3.8562022192443774E-4	0.0	0.0	19.921526284838375	0.0
24	3.8562022192443774E-4	0.0	0.0	20.486845530179604	0.0
25	3.8562022192443774E-4	0.0	0.0	20.950361036932776	0.0
26	3.8562022192443774E-4	0.0	0.0	21.403657607804952	0.0
27	3.8562022192443774E-4	0.0	0.0	21.872186177443144	0.0
28	3.8562022192443774E-4	0.0	0.0	22.346499050410202	0.0
29	3.8562022192443774E-4	0.0	0.0	22.882511158885173	0.0
30	3.8562022192443774E-4	0.0	0.0	23.48774209719558	0.0
31	3.8562022192443774E-4	0.0	0.0	24.019126763007453	0.0
32	3.8562022192443774E-4	0.0	0.0	24.544534315379497	0.0
33	5.784303328866566E-4	0.0	0.0	25.06859219697481	0.0
34	5.784303328866566E-4	0.0	0.0	25.588022635907027	0.0
35	5.784303328866566E-4	0.0	0.0	26.1296262375999	0.0
36	5.784303328866566E-4	0.0	0.0	26.647707005755382	0.0
37	5.784303328866566E-4	0.0	0.0	27.178320431123407	0.0
38	7.712404438488755E-4	0.0	0.0	27.724551475479373	0.0
39	7.712404438488755E-4	0.0	0.0	28.245331585188328	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	20	7.029703E-4	45.0	28
CCTAGCG	20	7.029703E-4	45.0	1
CGGCACC	20	7.029703E-4	45.0	14
GACCGCG	25	3.8878352E-5	45.0	20
CGACAAG	20	7.029703E-4	45.0	27
ACGGGTC	20	7.029703E-4	45.0	5
AACGGTA	20	7.029703E-4	45.0	19
TGACCGC	25	3.8878352E-5	45.0	19
CGGGCAA	45	3.8380676E-10	45.0	6
GTCGTTT	35	1.21021E-7	45.0	9
AATCTCG	20	7.029703E-4	45.0	18
GTCGTGG	25	3.8878352E-5	45.0	2
ATGACCG	25	3.8878352E-5	45.0	18
TCCCGGT	20	7.029703E-4	45.0	20
CGGTCTA	20	7.029703E-4	45.0	31
AGCTACG	35	1.21021E-7	45.0	9
CCCGGTA	20	7.029703E-4	45.0	21
CGGTTTT	500	0.0	43.65	1
CGGGAAC	100	0.0	42.75	6
TACGGGA	230	0.0	42.06522	4
>>END_MODULE