Basic Statistics
Measure | Value |
---|---|
Filename | SRR3549494_1.fastq |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 377503 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 42 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 2496 | 0.6611867985155084 | No Hit |
GAATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC | 921 | 0.24397157108685227 | No Hit |
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 893 | 0.23655441148812065 | No Hit |
GAATGATACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG | 884 | 0.2341703244742426 | No Hit |
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC | 716 | 0.1896673668818526 | No Hit |
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC | 483 | 0.12794600307812123 | TruSeq Adapter, Index 20 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTGCGG | 20 | 7.02726E-4 | 45.000004 | 2 |
CACGACT | 20 | 7.02726E-4 | 45.000004 | 12 |
TCGGCAA | 20 | 7.02726E-4 | 45.000004 | 21 |
AACGGGC | 20 | 7.02726E-4 | 45.000004 | 4 |
CCATACT | 20 | 7.02726E-4 | 45.000004 | 25 |
CGGGACG | 20 | 7.02726E-4 | 45.000004 | 6 |
CATACGA | 20 | 7.02726E-4 | 45.000004 | 18 |
TCGAGAA | 20 | 7.02726E-4 | 45.000004 | 38 |
AGGGCGC | 80 | 0.0 | 45.000004 | 6 |
CGGACGG | 20 | 7.02726E-4 | 45.000004 | 2 |
TCACGAC | 25 | 3.8858125E-5 | 45.0 | 25 |
CTCACGT | 25 | 3.8858125E-5 | 45.0 | 45 |
CGTATGA | 25 | 3.8858125E-5 | 45.0 | 15 |
CGACGGT | 25 | 3.8858125E-5 | 45.0 | 28 |
CACGAAA | 25 | 3.8858125E-5 | 45.0 | 35 |
GTATGAC | 25 | 3.8858125E-5 | 45.0 | 16 |
GCGATGC | 70 | 0.0 | 45.0 | 9 |
ATGACCG | 25 | 3.8858125E-5 | 45.0 | 18 |
CGGTCTA | 25 | 3.8858125E-5 | 45.0 | 31 |
GGCGCGA | 70 | 0.0 | 45.0 | 8 |