FastQCFastQC Report
Sat 18 Jun 2016
SRR3549494_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549494_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377503
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24960.6611867985155084No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC9210.24397157108685227No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8930.23655441148812065No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG8840.2341703244742426No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC7160.1896673668818526No Hit
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC4830.12794600307812123TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGG207.02726E-445.0000042
CACGACT207.02726E-445.00000412
TCGGCAA207.02726E-445.00000421
AACGGGC207.02726E-445.0000044
CCATACT207.02726E-445.00000425
CGGGACG207.02726E-445.0000046
CATACGA207.02726E-445.00000418
TCGAGAA207.02726E-445.00000438
AGGGCGC800.045.0000046
CGGACGG207.02726E-445.0000042
TCACGAC253.8858125E-545.025
CTCACGT253.8858125E-545.045
CGTATGA253.8858125E-545.015
CGACGGT253.8858125E-545.028
CACGAAA253.8858125E-545.035
GTATGAC253.8858125E-545.016
GCGATGC700.045.09
ATGACCG253.8858125E-545.018
CGGTCTA253.8858125E-545.031
GGCGCGA700.045.08