##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549494_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	377503
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.516284638797572	31.0	31.0	34.0	30.0	34.0
2	31.661388121418902	31.0	31.0	34.0	30.0	34.0
3	31.28928511826396	31.0	31.0	34.0	28.0	34.0
4	35.34649525964032	37.0	35.0	37.0	33.0	37.0
5	35.18805413466913	37.0	35.0	37.0	33.0	37.0
6	35.23399284244099	37.0	35.0	37.0	32.0	37.0
7	32.12011560173032	36.0	35.0	37.0	15.0	37.0
8	33.63391019409117	37.0	35.0	37.0	19.0	37.0
9	36.5642524695168	39.0	35.0	39.0	32.0	39.0
10	36.372023533587814	38.0	35.0	39.0	32.0	39.0
11	36.431954183145564	38.0	35.0	39.0	32.0	39.0
12	36.469567129267844	38.0	35.0	39.0	32.0	39.0
13	32.76575550392977	37.0	34.0	39.0	8.0	39.0
14	35.317584231118694	39.0	34.0	40.0	18.0	41.0
15	36.685530975912776	39.0	35.0	40.0	30.0	41.0
16	37.27869712293677	39.0	36.0	40.0	32.0	41.0
17	37.41301923428423	39.0	36.0	40.0	32.0	41.0
18	37.35342765487957	39.0	36.0	40.0	32.0	41.0
19	37.377342696614335	39.0	36.0	40.0	32.0	41.0
20	37.32886361167991	39.0	35.0	40.0	32.0	41.0
21	37.26569590175442	39.0	35.0	40.0	32.0	41.0
22	37.25771450822907	39.0	35.0	40.0	32.0	41.0
23	37.168817201452704	39.0	35.0	40.0	32.0	41.0
24	37.07640733980922	39.0	35.0	40.0	32.0	41.0
25	36.896345194607726	39.0	35.0	40.0	31.0	41.0
26	36.762481357764045	39.0	35.0	40.0	31.0	41.0
27	36.73440740868284	39.0	35.0	40.0	31.0	41.0
28	36.594895934601844	38.0	35.0	40.0	31.0	41.0
29	36.56994249052325	38.0	35.0	40.0	31.0	41.0
30	36.41493180186647	38.0	35.0	40.0	30.0	41.0
31	36.32103056134653	38.0	35.0	40.0	30.0	41.0
32	36.13323337827779	38.0	35.0	40.0	30.0	41.0
33	35.92347345584009	38.0	35.0	40.0	29.0	41.0
34	35.809731313393534	38.0	35.0	40.0	29.0	41.0
35	35.64464653261034	38.0	35.0	40.0	27.0	41.0
36	35.48798817492841	38.0	35.0	40.0	27.0	41.0
37	35.4013213140028	38.0	34.0	40.0	26.0	41.0
38	35.284297078433816	38.0	34.0	40.0	25.0	41.0
39	35.158364304389636	38.0	34.0	40.0	25.0	41.0
40	35.081281473259814	38.0	34.0	40.0	24.0	41.0
41	35.07306432001865	38.0	34.0	40.0	25.0	41.0
42	35.07647091546292	38.0	34.0	40.0	25.0	41.0
43	35.018683295232094	38.0	34.0	40.0	24.0	41.0
44	34.82600932972718	38.0	34.0	40.0	23.0	41.0
45	34.76453167259598	38.0	34.0	40.0	23.0	41.0
46	34.71611881230083	38.0	34.0	40.0	23.0	41.0
47	34.53569640506168	38.0	33.0	40.0	23.0	41.0
48	34.42069334548335	38.0	33.0	40.0	23.0	41.0
49	34.35688193206411	38.0	33.0	40.0	23.0	41.0
50	34.16665562922679	37.0	33.0	40.0	22.0	41.0
51	32.304924729075	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	8.0
10	16.0
11	10.0
12	7.0
13	7.0
14	4.0
15	13.0
16	41.0
17	79.0
18	165.0
19	348.0
20	585.0
21	1002.0
22	1540.0
23	2269.0
24	3243.0
25	4580.0
26	5610.0
27	6497.0
28	6780.0
29	7900.0
30	9620.0
31	12018.0
32	15640.0
33	21047.0
34	28859.0
35	35414.0
36	40554.0
37	56133.0
38	64467.0
39	53037.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.83336556265778	23.187895195534868	26.908395429970096	15.070343811837256
2	32.56689350813106	26.30575121257315	28.072889486970965	13.054465792324828
3	29.978569706730806	26.51290188422344	30.233137220101565	13.275391188944194
4	26.486941825627873	27.34494825206687	31.841336360240845	14.326773562064407
5	23.60484552440643	32.542787739435184	28.899902782229546	14.952463953928843
6	20.596127712892347	42.8091962183082	26.85223693586541	9.742439132934043
7	74.8500011920435	13.717241982182923	9.213966511524411	2.2187903142491585
8	80.86902620641425	5.973992259664162	9.448931531669947	3.708050002251638
9	73.78404939828293	8.417416550332051	12.293942034897736	5.504592016487287
10	30.865714974450537	36.215606233592844	19.040378487058383	13.87830030489824
11	27.27210114886504	27.15872456642729	30.351546875124168	15.2176274095835
12	26.32482390868417	24.200072582204648	31.976964421474797	17.49813908763639
13	19.70898244517262	34.98038426184692	30.19949510335017	15.11113818963028
14	17.487808043909585	32.74517023705772	31.98120279838836	17.78581892064434
15	17.09337409239132	27.145214740015312	39.97451675880722	15.786894408786157
16	19.344217132049284	26.470783013644926	35.703292424166165	18.481707430139625
17	17.986876925481386	25.797940678617127	32.80901078931823	23.40617160658326
18	19.107132923446965	27.24190271335592	34.5056860475281	19.145278315669014
19	23.23981531272599	27.142565754444338	31.259884027411704	18.35773490541797
20	24.076364956040084	26.341777416338413	31.282665303322094	18.29919232429941
21	21.43373695043483	28.37646323340477	31.943587203280504	18.246212612879898
22	19.345011827720572	26.118997729819366	31.045051297605582	23.49093914485448
23	18.384489659684824	29.188112412351693	31.566636556530675	20.860761371432808
24	18.341840991992118	26.784422905248434	34.8749546361221	19.998781466637354
25	18.53998511270109	29.35446870620896	31.76133699599738	20.34420918509257
26	17.334431779350098	30.832072857699146	31.42200194435541	20.41149341859535
27	19.450441453445404	29.530361348121737	30.789953987120633	20.229243211312227
28	17.458404304071756	29.480295520830296	34.109132907553054	18.952167267544894
29	18.90686961428121	26.886143951173896	32.65007165505969	21.556914779485194
30	19.56143394886928	28.502290048026108	32.44080179495262	19.49547420815199
31	22.323796102282632	27.88375191720331	29.698042134764492	20.09440984574957
32	22.23399549142656	28.88162478178981	29.5131429419104	19.371236784873233
33	20.74049742651052	28.586792687740232	29.6100958138081	21.062614071941148
34	19.57335438393867	27.518191908408674	31.174851590583387	21.733602117069267
35	19.840107230935914	27.94176470120767	30.84982106102468	21.36830700683173
36	21.49227953155339	28.44454216257884	30.509956212268513	19.553222093599256
37	19.714280416314573	30.46439366044773	31.10306408160995	18.718261841627747
38	19.178125736749113	30.54598241603378	30.03605269362098	20.239839153596133
39	19.35163429164801	29.052749249674836	30.621213606249487	20.974402852427662
40	19.63401615351401	27.886400902774284	32.94675803900896	19.532824904702746
41	17.534165291401656	27.93037406325248	31.24187092552907	23.293589719816797
42	20.312156459683763	27.578058982312726	29.89353726990249	22.21624728810102
43	20.48831400015364	26.670516525696485	30.99260138330027	21.84856809084961
44	19.216800926085355	28.504409236482893	31.028892485622634	21.249897351809125
45	17.76489193463363	29.459103636262498	31.009819789511607	21.766184639592268
46	19.87533873902989	29.403210040714907	30.329825193442172	20.391626026813032
47	18.810976336611894	27.99183052849911	31.730343864816966	21.466849270072025
48	19.059716081726503	27.859646148507426	31.500411917256287	21.580225852509784
49	19.845670100634962	26.755284063967704	31.963984392177014	21.43506144322032
50	18.31641073051075	27.796865190475305	32.11471167116552	21.772012407848415
51	18.23084849656824	26.985216011528383	30.497770878642022	24.286164613261352
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	339.0
1	481.0
2	623.0
3	2697.5
4	4772.0
5	4144.5
6	3517.0
7	2843.5
8	2170.0
9	2111.5
10	2053.0
11	2016.0
12	1979.0
13	1943.0
14	1907.0
15	1869.0
16	1831.0
17	1926.5
18	2022.0
19	2016.0
20	2010.0
21	2195.0
22	2380.0
23	2610.5
24	2841.0
25	3361.5
26	4598.0
27	5314.0
28	6325.0
29	7336.0
30	8577.5
31	9819.0
32	11221.5
33	12624.0
34	13682.0
35	14740.0
36	16327.0
37	17914.0
38	19045.0
39	20176.0
40	22772.0
41	25368.0
42	27775.5
43	30183.0
44	31537.0
45	32891.0
46	36398.0
47	39905.0
48	37728.5
49	35552.0
50	32265.0
51	28978.0
52	24991.5
53	21005.0
54	17705.5
55	14406.0
56	12110.5
57	9815.0
58	8392.5
59	6970.0
60	5831.5
61	4693.0
62	3805.5
63	2918.0
64	2238.0
65	1558.0
66	1230.5
67	903.0
68	786.5
69	670.0
70	574.5
71	479.0
72	456.0
73	433.0
74	342.5
75	186.0
76	120.0
77	92.0
78	64.0
79	50.5
80	37.0
81	34.0
82	31.0
83	22.0
84	13.0
85	10.0
86	7.0
87	4.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	377503.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.45255270597769
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.37929199054528	37.49224223523132
2	9.368289112600705	9.07835043986245
3	3.7287315921578106	5.419996919864112
4	2.0029670893977203	3.8819547386952693
5	1.206114028603617	2.921965177016791
6	0.8754042102658063	2.5449341182163248
7	0.6894519381239368	2.3383994459131965
8	0.5755592828844033	2.2309853191497013
9	0.492758207356699	2.148785371192819
>10	3.635070885467842	27.853523391126277
>50	0.028032633197696957	0.9149921132996385
>100	0.015633583898715515	1.6301643674287805
>500	0.0021563563998228297	0.8917450970039826
>1k	5.390890999557074E-4	0.6519612659993969
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2496	0.6611867985155084	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTC	921	0.24397157108685227	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	893	0.23655441148812065	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCG	884	0.2341703244742426	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGC	716	0.1896673668818526	No Hit
GCTGTCTCTTATACACATCTGACGCCGGATTACTCGTATGCCGTCTTCTGC	483	0.12794600307812123	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.648985570975595E-4	0.0	0.0	0.13112478576329195	0.0
2	2.648985570975595E-4	0.0	0.0	0.4707247359623632	0.0
3	2.648985570975595E-4	0.0	0.0	0.6307234644492892	0.0
4	2.648985570975595E-4	0.0	0.0	0.9226416743707997	0.0
5	2.648985570975595E-4	0.0	0.0	1.7411782158022586	0.0
6	2.648985570975595E-4	0.0	0.0	2.286869243423231	0.0
7	2.648985570975595E-4	0.0	0.0	2.706733456422863	0.0
8	2.648985570975595E-4	0.0	0.0	3.8518899187556124	0.0
9	2.648985570975595E-4	0.0	0.0	4.341157553714805	0.0
10	2.648985570975595E-4	0.0	0.0	5.565783583176822	0.0
11	2.648985570975595E-4	0.0	0.0	6.38882340007894	0.0
12	2.648985570975595E-4	0.0	0.0	7.403914670876787	0.0
13	2.648985570975595E-4	0.0	0.0	7.767355491214639	0.0
14	2.648985570975595E-4	0.0	0.0	7.933446886514809	0.0
15	2.648985570975595E-4	0.0	0.0	8.228014082007295	0.0
16	2.648985570975595E-4	0.0	0.0	8.780327573555708	0.0
17	2.648985570975595E-4	0.0	0.0	9.390653849108483	0.0
18	2.648985570975595E-4	0.0	0.0	10.274090537028846	0.0
19	2.648985570975595E-4	0.0	0.0	10.623491733840526	0.0
20	2.648985570975595E-4	0.0	0.0	11.058455164594719	0.0
21	2.648985570975595E-4	0.0	0.0	11.550371785124886	0.0
22	2.648985570975595E-4	0.0	0.0	12.082023189219688	0.0
23	2.648985570975595E-4	0.0	0.0	12.632747289425515	0.0
24	2.648985570975595E-4	0.0	0.0	13.079366256692	0.0
25	2.648985570975595E-4	0.0	0.0	13.419496004005266	0.0
26	2.648985570975595E-4	0.0	0.0	13.760420446989825	0.0
27	2.648985570975595E-4	0.0	0.0	14.133927412497384	0.0
28	2.648985570975595E-4	0.0	0.0	14.524652784216284	0.0
29	2.648985570975595E-4	0.0	0.0	14.926503895333282	0.0
30	2.648985570975595E-4	0.0	0.0	15.420274805763134	0.0
31	2.648985570975595E-4	0.0	0.0	15.86133090333057	0.0
32	2.648985570975595E-4	0.0	0.0	16.291526160057007	0.0
33	2.648985570975595E-4	0.0	0.0	16.72066182255505	0.0
34	2.648985570975595E-4	0.0	0.0	17.161982818679586	0.0
35	2.648985570975595E-4	0.0	0.0	17.64012471424068	0.0
36	2.648985570975595E-4	0.0	0.0	18.118531508358874	0.0
37	2.648985570975595E-4	0.0	0.0	18.595613809691578	0.0
38	2.648985570975595E-4	0.0	0.0	19.05256382068487	0.0
39	2.648985570975595E-4	0.0	0.0	19.477461106269356	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGG	20	7.02726E-4	45.000004	2
CACGACT	20	7.02726E-4	45.000004	12
TCGGCAA	20	7.02726E-4	45.000004	21
AACGGGC	20	7.02726E-4	45.000004	4
CCATACT	20	7.02726E-4	45.000004	25
CGGGACG	20	7.02726E-4	45.000004	6
CATACGA	20	7.02726E-4	45.000004	18
TCGAGAA	20	7.02726E-4	45.000004	38
AGGGCGC	80	0.0	45.000004	6
CGGACGG	20	7.02726E-4	45.000004	2
TCACGAC	25	3.8858125E-5	45.0	25
CTCACGT	25	3.8858125E-5	45.0	45
CGTATGA	25	3.8858125E-5	45.0	15
CGACGGT	25	3.8858125E-5	45.0	28
CACGAAA	25	3.8858125E-5	45.0	35
GTATGAC	25	3.8858125E-5	45.0	16
GCGATGC	70	0.0	45.0	9
ATGACCG	25	3.8858125E-5	45.0	18
CGGTCTA	25	3.8858125E-5	45.0	31
GGCGCGA	70	0.0	45.0	8
>>END_MODULE