FastQCFastQC Report
Sat 18 Jun 2016
SRR3549493_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549493_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences470348
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42560.9048619320162944No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17780.37801797817785976No Hit
GAATCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTC13200.28064326838851233No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGC9510.20219071836172367No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCG9190.1953872451886688No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5760.12246251711498721No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4810.10226470613248063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAG302.1627566E-645.0000041
GTTTACG302.1627566E-645.0000041
TTAACGG302.1627566E-645.0000042
CTAGACG207.029032E-445.01
CGGGTAG253.887279E-545.06
TTGTGCG207.029032E-445.01
ACGGGTA406.7993824E-945.05
GTTACGG207.029032E-445.02
TGACCGG207.029032E-445.02
TTGCGAA207.029032E-445.01
CGATGCG207.029032E-445.012
CACACCG253.887279E-545.043
CGCCGGT253.887279E-545.028
GGCGAAA207.029032E-445.08
CGGTTTT8400.042.8571431
CATACGA950.042.6315818
CGTTTTT25600.041.3085941
CGAAAAA1000.040.522
TACGGGT501.0786607E-940.54
AACGGGC501.0786607E-940.54