##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549493_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	470348
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.624180394091184	31.0	31.0	34.0	30.0	34.0
2	31.724527371223008	31.0	31.0	34.0	30.0	34.0
3	31.141235850901886	31.0	31.0	34.0	28.0	34.0
4	35.3486184697288	37.0	35.0	37.0	33.0	37.0
5	35.29213263370951	37.0	35.0	37.0	33.0	37.0
6	35.363169397977664	37.0	35.0	37.0	33.0	37.0
7	32.052442021652055	37.0	35.0	37.0	10.0	37.0
8	33.652038490649474	37.0	35.0	37.0	18.0	37.0
9	36.681225390561885	39.0	35.0	39.0	32.0	39.0
10	36.55068374905389	38.0	35.0	39.0	32.0	39.0
11	36.60760755865869	38.0	35.0	39.0	32.0	39.0
12	36.69852747327511	39.0	35.0	39.0	32.0	39.0
13	33.05804213050762	38.0	35.0	39.0	10.0	39.0
14	35.692387338736424	39.0	35.0	41.0	18.0	41.0
15	37.039073196867	39.0	35.0	41.0	31.0	41.0
16	37.56698869773019	39.0	36.0	41.0	32.0	41.0
17	37.67462814766939	39.0	36.0	41.0	33.0	41.0
18	37.591013462372544	39.0	36.0	40.0	33.0	41.0
19	37.580499970234804	39.0	36.0	40.0	33.0	41.0
20	37.481101227176474	39.0	35.0	41.0	33.0	41.0
21	37.43988918843069	39.0	35.0	41.0	33.0	41.0
22	37.42814894503644	39.0	35.0	41.0	33.0	41.0
23	37.31533885548573	39.0	35.0	40.0	32.0	41.0
24	37.189478853954945	39.0	35.0	40.0	32.0	41.0
25	36.99425319125414	39.0	35.0	40.0	32.0	41.0
26	36.878885421007425	39.0	35.0	40.0	31.0	41.0
27	36.884132599692144	39.0	35.0	40.0	31.0	41.0
28	36.718321328037966	39.0	35.0	40.0	31.0	41.0
29	36.658191381700355	39.0	35.0	40.0	31.0	41.0
30	36.50881262384447	38.0	35.0	40.0	31.0	41.0
31	36.4040412630648	38.0	35.0	40.0	30.0	41.0
32	36.21424349630487	38.0	35.0	40.0	30.0	41.0
33	35.947668534787006	38.0	35.0	40.0	29.0	41.0
34	35.667429222618146	38.0	35.0	40.0	27.0	41.0
35	35.48575948021465	38.0	35.0	40.0	25.0	41.0
36	35.258823254271306	38.0	34.0	40.0	25.0	41.0
37	35.18531172663645	38.0	34.0	40.0	24.0	41.0
38	35.05523994999447	38.0	34.0	40.0	24.0	41.0
39	34.88733448425421	38.0	34.0	40.0	23.0	41.0
40	34.82877571500251	38.0	34.0	40.0	23.0	41.0
41	34.7362782450441	38.0	34.0	40.0	23.0	41.0
42	34.71670125098863	38.0	34.0	40.0	23.0	41.0
43	34.67509588645003	38.0	34.0	40.0	23.0	41.0
44	34.50366111900125	38.0	34.0	40.0	22.0	41.0
45	34.451750618690845	38.0	33.0	40.0	22.0	41.0
46	34.35723549371954	38.0	33.0	40.0	22.0	41.0
47	34.18168037283033	38.0	33.0	40.0	20.0	41.0
48	34.08450551506544	38.0	33.0	40.0	20.0	41.0
49	33.978658355090275	37.0	33.0	40.0	20.0	41.0
50	33.842040361604596	37.0	33.0	40.0	20.0	41.0
51	32.028368357046276	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	11.0
10	13.0
11	8.0
12	7.0
13	8.0
14	7.0
15	21.0
16	53.0
17	104.0
18	217.0
19	436.0
20	755.0
21	1271.0
22	1935.0
23	3084.0
24	4803.0
25	7005.0
26	8774.0
27	9377.0
28	9330.0
29	10037.0
30	11224.0
31	13882.0
32	17907.0
33	23652.0
34	32750.0
35	39777.0
36	48770.0
37	70037.0
38	81795.0
39	73290.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.016404874688526	22.318155918596442	26.486133671239166	18.17930553547586
2	34.976868191211615	25.97693622594334	26.667913970081724	12.378281612763315
3	30.620519275089936	25.668228630715976	31.297890072882208	12.41336202131188
4	25.3631353806118	26.602643149327733	33.646576577342735	14.387644892717733
5	22.97830542491942	31.341262214360434	31.410997814384245	14.269434546335905
6	20.565198533851532	40.307814639373404	29.324882852696298	9.802103974078767
7	73.70393836053304	13.394550417988382	11.027154362301955	1.8743568591766095
8	81.00938028863735	4.990985398045702	11.365839761198092	2.6337945521188564
9	74.65089678280762	7.020546488982625	13.364785222856268	4.963771505353483
10	29.671647375985437	36.85568982965804	20.438696454540043	13.033966339816477
11	24.559900329118015	27.405240375211548	32.28354324882853	15.751316046841913
12	23.385025555546108	24.20952146070569	35.29280447668535	17.112648507062854
13	19.54744146886986	33.09634568447192	32.35008972080247	15.00612312585575
14	18.43422317092876	29.68567953940487	34.37476081539626	17.505336474270113
15	18.41232449165299	26.3281655285023	38.47342818508849	16.78608179475622
16	20.32431306181806	26.799518654273008	34.94965429851939	17.92651398538954
17	19.67755789330453	25.699482085604703	34.274409586093704	20.348550434997065
18	21.008062115710068	25.833000246625904	34.88608434605867	18.272853291605365
19	23.02146495786099	26.454454999277132	32.394737513500644	18.12934252936124
20	23.75942918860078	26.03604139913426	32.794016345344296	17.410513066920664
21	22.587318326005427	26.869679471370134	33.03511442591443	17.50788777671001
22	21.13328854380161	24.632187231581725	33.105700460084876	21.12882376453179
23	19.349715529777953	27.815149633888097	33.220296461343516	19.61483837499043
24	19.113507445550955	26.459557604156924	35.25262146325699	19.17431348703513
25	19.713913953073046	28.956007041594734	32.67197904530263	18.658099960029595
26	18.973185811356696	28.303511442591443	32.36879927202837	20.35450347402349
27	19.637800096949494	27.194970532456818	32.83271109901605	20.33451827157764
28	18.274128942825314	28.0473181559186	34.783819639926186	18.89473326132991
29	20.15805318615153	27.083776267784703	32.91010060635955	19.848069939704217
30	19.474091523722862	27.351025198363764	33.81793905788905	19.35694422002432
31	22.06260045753357	27.362506059343296	31.469677770501843	19.105215712621295
32	21.667148579349757	27.664410181397603	32.025436485325756	18.643004753926878
33	20.319635674011582	27.86086046926956	30.86416865809996	20.955335198618897
34	19.997108523901453	28.22059411329484	32.73512378069004	19.04717358211367
35	20.003274171464533	27.234090503201884	32.49019874646007	20.272436578873513
36	20.474202080161923	30.025002763910972	30.401745090868886	19.09905006505821
37	19.98987983365508	29.56853223570633	31.132268022825656	19.309319907812938
38	20.05791456538563	29.477535782016716	30.432998545757613	20.03155110684004
39	20.936413038856337	28.18678935596622	30.66559228486142	20.211205320316022
40	20.618775885089338	27.729468393614944	32.39984011838043	19.251915602915286
41	18.780137260071267	29.14033864287719	31.094211094763878	20.98531300228767
42	20.227363569102028	28.771037614702305	30.85885344468351	20.14274537151216
43	20.519912915543383	27.925280855876927	30.938794254466906	20.616011974112787
44	20.55286723872537	28.292030581611915	30.316276459132386	20.83882572053033
45	19.78322433602354	28.92943097451249	30.554823237262625	20.73252145220135
46	20.28030309472986	29.124818219701158	29.961432811450244	20.63344587411874
47	19.33525814928521	28.720862000051028	31.762227116943198	20.181652733720565
48	19.69477918477383	28.241642358423974	31.201153188702836	20.862425268099365
49	19.978611581212206	27.809621811934992	32.01140432190633	20.200362284946465
50	19.280617755364112	27.879570020495464	32.198074617092026	20.641737607048398
51	19.13072873702025	27.197309226360055	31.261108796040375	22.410853240579314
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	251.0
1	466.0
2	681.0
3	4625.5
4	8570.0
5	7734.5
6	6899.0
7	5320.5
8	3742.0
9	3614.5
10	3487.0
11	3408.0
12	3329.0
13	3252.5
14	3176.0
15	3099.0
16	3022.0
17	2920.0
18	2818.0
19	2907.0
20	2996.0
21	3114.0
22	3232.0
23	3648.0
24	4064.0
25	4807.0
26	6705.0
27	7860.0
28	9155.5
29	10451.0
30	11764.0
31	13077.0
32	14471.5
33	15866.0
34	17411.0
35	18956.0
36	20038.0
37	21120.0
38	22682.0
39	24244.0
40	26872.0
41	29500.0
42	31047.0
43	32594.0
44	34240.0
45	35886.0
46	38865.5
47	41845.0
48	39100.5
49	36356.0
50	34335.5
51	32315.0
52	28991.0
53	25667.0
54	22296.0
55	18925.0
56	16791.5
57	14658.0
58	12921.5
59	11185.0
60	9849.0
61	8513.0
62	7262.5
63	6012.0
64	5043.0
65	4074.0
66	3481.0
67	2888.0
68	2439.0
69	1990.0
70	1774.0
71	1558.0
72	1395.5
73	1233.0
74	1016.5
75	613.5
76	427.0
77	344.0
78	261.0
79	199.5
80	138.0
81	96.5
82	55.0
83	45.5
84	36.0
85	27.5
86	19.0
87	15.0
88	11.0
89	9.5
90	8.0
91	4.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	470348.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.226661864689305
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.80860305198365	40.88265542947078
2	9.93936078179562	10.580779909675792
3	4.048253647303725	6.464250840825918
4	2.1286817518517034	4.532104952933804
5	1.4201979260919186	3.7796197396513778
6	1.0963374809014244	3.5012630631315234
7	0.8515517111288728	3.1727678491987876
8	0.6963628206150203	2.965205471041359
9	0.6270410595097314	3.00377722048209
>10	2.351823927577874	17.166270619727477
>50	0.01976924273627034	0.7347186454192833
>100	0.009690805245335443	1.194614139600903
>500	0.0011628966294402532	0.5046677349678426
>1k	0.0011628966294402532	1.517304383873064
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4256	0.9048619320162944	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1778	0.37801797817785976	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTC	1320	0.28064326838851233	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGC	951	0.20219071836172367	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCG	919	0.1953872451886688	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	576	0.12246251711498721	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	481	0.10226470613248063	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06548342929065287	0.0
2	0.0	0.0	0.0	0.27766674887530085	0.0
3	0.0	0.0	0.0	0.38141971476438724	0.0
4	0.0	0.0	0.0	0.6146512794781737	0.0
5	0.0	0.0	0.0	1.236106032129402	0.0
6	0.0	0.0	0.0	1.6349596468997423	0.0
7	0.0	0.0	0.0	1.937501594564025	0.0
8	0.0	0.0	0.0	2.5878711082007366	0.0
9	0.0	0.0	0.0	2.904232610747787	0.0
10	0.0	0.0	0.0	3.702577665898441	0.0
11	0.0	0.0	0.0	4.111849098965021	0.0
12	0.0	0.0	0.0	4.74478471259578	0.0
13	0.0	0.0	0.0	4.9644093309634565	0.0
14	0.0	0.0	0.0	5.074540552952282	0.0
15	0.0	0.0	0.0	5.227618699346016	0.0
16	0.0	0.0	0.0	5.484449811628837	0.0
17	0.0	0.0	0.0	5.7431944007415785	0.0
18	0.0	0.0	0.0	6.163521477714373	0.0
19	0.0	0.0	0.0	6.355081769243198	0.0
20	0.0	0.0	0.0	6.595754632740014	0.0
21	0.0	0.0	0.0	6.848333574289675	0.0
22	0.0	0.0	0.0	7.11303120242884	0.0
23	0.0	0.0	0.0	7.39707620740388	0.0
24	0.0	0.0	0.0	7.637536462364037	0.0
25	0.0	0.0	0.0	7.835262401455943	0.0
26	0.0	0.0	0.0	8.02788573566806	0.0
27	0.0	0.0	0.0	8.243045574765919	0.0
28	0.0	0.0	0.0	8.461607150450305	0.0
29	0.0	0.0	0.0	8.708020444436885	0.0
30	0.0	0.0	0.0	9.013751520151038	0.0
31	0.0	0.0	0.0	9.266968287310672	0.0
32	0.0	0.0	0.0	9.559942850825346	0.0
33	0.0	0.0	0.0	9.8550434997066	0.0
34	0.0	0.0	0.0	10.124843732725557	0.0
35	0.0	0.0	0.0	10.424621769413285	0.0
36	0.0	0.0	0.0	10.69761113048211	0.0
37	0.0	0.0	0.0	10.983357003750415	0.0
38	0.0	0.0	0.0	11.27952069531496	0.0
39	0.0	0.0	0.0	11.587590464932347	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	30	2.1627566E-6	45.000004	1
GTTTACG	30	2.1627566E-6	45.000004	1
TTAACGG	30	2.1627566E-6	45.000004	2
CTAGACG	20	7.029032E-4	45.0	1
CGGGTAG	25	3.887279E-5	45.0	6
TTGTGCG	20	7.029032E-4	45.0	1
ACGGGTA	40	6.7993824E-9	45.0	5
GTTACGG	20	7.029032E-4	45.0	2
TGACCGG	20	7.029032E-4	45.0	2
TTGCGAA	20	7.029032E-4	45.0	1
CGATGCG	20	7.029032E-4	45.0	12
CACACCG	25	3.887279E-5	45.0	43
CGCCGGT	25	3.887279E-5	45.0	28
GGCGAAA	20	7.029032E-4	45.0	8
CGGTTTT	840	0.0	42.857143	1
CATACGA	95	0.0	42.63158	18
CGTTTTT	2560	0.0	41.308594	1
CGAAAAA	100	0.0	40.5	22
TACGGGT	50	1.0786607E-9	40.5	4
AACGGGC	50	1.0786607E-9	40.5	4
>>END_MODULE