FastQCFastQC Report
Sat 18 Jun 2016
SRR3549490_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549490_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199795
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15580.7797992942766335No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT7210.3608698916389299No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC7180.35936835256137545No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6500.3253334668034736No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC6270.3138216672088891No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG5920.2963037113040867No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5540.2772842163217298No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC5500.2752821642183238No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC5460.2732801121149178No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3220.1611651943241823No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC2500.12512825646287445No Hit
ACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC2070.10360619635126005No Hit
GAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT2000.10010260517029955No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAATC253.879201E-545.0000048
TTTACGG253.879201E-545.0000042
TTAGCGG351.2051896E-745.0000042
GTCCTGC302.156472E-645.00000414
CTCCACT302.156472E-645.0000045
TAAGTAG253.879201E-545.0000041
CAGGGCC253.879201E-545.0000045
AACGGGA302.156472E-645.0000044
AACATTG253.879201E-545.00000415
TCCACTC302.156472E-645.0000046
CGGGATT600.045.0000046
TAGGGTC302.156472E-645.0000045
CTCTGTG302.156472E-645.00000418
TCATAAT253.879201E-545.00000444
GGCGATC253.879201E-545.0000048
TGGGACC502.1827873E-1145.0000046
CTCTAGT253.879201E-545.00000410
AGTACGG302.156472E-645.0000042
TGGTACC302.156472E-645.00000415
TCAGTAG253.879201E-545.0000041
Produced by FastQC (version 0.11.5)