##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549490_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	199795
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.08361570609875	31.0	31.0	33.0	28.0	34.0
2	31.188428138842312	31.0	31.0	34.0	28.0	34.0
3	30.741349883630722	31.0	31.0	34.0	27.0	34.0
4	34.923026101754296	35.0	35.0	37.0	32.0	37.0
5	34.77316249155384	35.0	35.0	37.0	32.0	37.0
6	34.798323281363395	35.0	35.0	37.0	32.0	37.0
7	32.003488575790186	35.0	35.0	37.0	17.0	37.0
8	33.44310918691659	35.0	35.0	37.0	19.0	37.0
9	36.25517155083961	39.0	35.0	39.0	32.0	39.0
10	36.04496108511224	37.0	35.0	39.0	32.0	39.0
11	36.08018218674141	37.0	35.0	39.0	31.0	39.0
12	36.05549187917615	37.0	35.0	39.0	32.0	39.0
13	32.38541004529643	37.0	33.0	39.0	11.0	39.0
14	34.65199829825571	38.0	33.0	40.0	18.0	41.0
15	36.00378387847544	38.0	33.0	40.0	27.0	41.0
16	36.629650391651445	38.0	35.0	40.0	31.0	41.0
17	36.79839835831728	38.0	35.0	40.0	32.0	41.0
18	36.7278760729748	38.0	35.0	40.0	31.0	41.0
19	36.65574213568908	38.0	35.0	40.0	31.0	41.0
20	36.51513301133662	38.0	35.0	40.0	31.0	41.0
21	36.36045446582747	38.0	35.0	40.0	31.0	41.0
22	36.425656297705146	38.0	35.0	40.0	31.0	41.0
23	36.25618759228209	38.0	35.0	40.0	31.0	41.0
24	36.1977076503416	38.0	35.0	40.0	31.0	41.0
25	36.01333867213894	38.0	34.0	40.0	30.0	41.0
26	35.928827047723914	38.0	34.0	40.0	30.0	41.0
27	35.887619810305566	38.0	34.0	40.0	30.0	41.0
28	35.70371130408669	38.0	34.0	40.0	29.0	41.0
29	35.70072824645261	38.0	34.0	40.0	30.0	41.0
30	35.447038214169524	37.0	34.0	40.0	28.0	41.0
31	35.25487624815436	37.0	34.0	40.0	27.0	41.0
32	35.00334342701269	37.0	34.0	40.0	26.0	41.0
33	34.787607297479916	37.0	34.0	40.0	25.0	41.0
34	34.59141620160665	37.0	34.0	40.0	24.0	41.0
35	34.38153607447634	37.0	33.0	40.0	23.0	41.0
36	34.078645611752044	37.0	33.0	40.0	22.0	41.0
37	33.969658900372885	37.0	33.0	40.0	21.0	41.0
38	33.67876072974799	37.0	33.0	40.0	19.0	41.0
39	33.60101103631222	37.0	33.0	40.0	19.0	41.0
40	33.52615430816587	37.0	33.0	40.0	18.0	41.0
41	33.500763282364424	37.0	33.0	40.0	18.0	41.0
42	33.417212642959036	37.0	32.0	40.0	18.0	41.0
43	33.32447258439901	37.0	32.0	40.0	18.0	41.0
44	33.01603643734828	36.0	31.0	40.0	15.0	41.0
45	33.04516629545284	36.0	31.0	40.0	15.0	41.0
46	32.85700843364449	36.0	31.0	40.0	15.0	41.0
47	32.70031282064116	36.0	31.0	39.0	15.0	41.0
48	32.557806751920715	36.0	31.0	39.0	15.0	40.0
49	32.48705423058635	36.0	31.0	39.0	14.0	40.0
50	32.13297129557797	35.0	30.0	39.0	12.0	40.0
51	29.872133937285717	34.0	26.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	5.0
10	2.0
11	4.0
12	4.0
13	2.0
14	3.0
15	11.0
16	35.0
17	81.0
18	185.0
19	354.0
20	615.0
21	964.0
22	1550.0
23	2057.0
24	2726.0
25	3572.0
26	4185.0
27	4680.0
28	4925.0
29	5593.0
30	6567.0
31	8131.0
32	10326.0
33	13433.0
34	18289.0
35	20992.0
36	21812.0
37	26818.0
38	26800.0
39	15073.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.482794864736356	24.206311469255986	25.993643484571688	17.317250181435973
2	29.665407042218273	28.065266898571036	28.748467178858327	13.52085888035236
3	27.10127881078105	28.366575740133637	32.04034134988363	12.491804099201682
4	23.654746114767637	27.791986786456118	34.39425411046322	14.159012988313021
5	20.01952000800821	31.88217923371456	32.24555169048274	15.85274906779449
6	18.477939888385595	40.20420931454741	30.350108861583124	10.967741935483872
7	68.72794614479841	14.687554743612202	12.585900548061762	3.998598563527616
8	71.07284967091269	7.482169223454041	13.421757301233765	8.02322380439951
9	64.44105207838035	8.949172902224781	18.69616356765685	7.913611451738031
10	31.80510022773343	31.998298255712104	21.672214019369854	14.524387497184616
11	26.43159238219175	25.383518106058713	32.84116219124603	15.343727320503517
12	25.781926474636503	19.390375134512876	35.355239120098105	19.47245927075252
13	19.00648164368478	34.4192797617558	34.33419254736105	12.240046047198378
14	14.39225205835982	34.27112790610376	31.99379363847944	19.342826397056985
15	11.452238544508122	24.38099051527816	48.70342100653169	15.463349933682025
16	12.16396806726895	25.560199204184286	41.027553242073125	21.24827948647364
17	13.029355088966192	25.23336419830326	33.974824194799666	27.76245651793088
18	15.530919192171977	23.998098050501763	40.00300307815511	20.46797967917115
19	18.502465026652317	28.801521559598587	33.259090567832025	19.436922845917064
20	19.41840386396056	26.558722690758024	33.08941665206837	20.933456793213043
21	16.261167696889313	32.20300808328537	33.49433168998223	18.04149252984309
22	14.814184539152631	26.699366851022297	30.10836107009685	28.37808753972822
23	13.006832002802874	31.8466428088791	31.412197502440005	23.734327685878025
24	15.472859681173205	25.146274931805102	36.10050301559098	23.280362371430716
25	13.134963337420857	28.95868264971596	33.75610000250256	24.15025401036062
26	12.22703270852624	28.675892790109863	34.92629945694337	24.17077504442053
27	17.200130133386722	29.69393628469181	30.626892564879	22.47904101704247
28	13.74108461172702	27.017192622437996	38.22768337545985	21.01403939037513
29	16.00940964488601	24.20731249530769	32.365174303661256	27.418103556145045
30	17.99144122725794	26.623789384118723	31.73252583898496	23.65224354963838
31	18.835306188843564	26.40906929602843	29.580820340849375	25.174804174278638
32	21.010535799194173	24.23434019870367	28.871593383217796	25.883530618884354
33	17.26619785279912	23.902500062564126	31.277559498485946	27.553742586150804
34	15.481368402612677	21.205235366250406	37.14207062238795	26.17132560874897
35	18.127080257263696	20.229234965839986	31.63242323381466	30.01126154308166
36	19.24522635701594	19.60259265747391	38.29725468605321	22.85492629945694
37	18.691658950424184	21.96801721764809	38.42738807277459	20.912935759153132
38	17.181611151430214	24.105207838033984	33.999849846092246	24.713331164443556
39	20.04604719837834	20.923446532696012	37.93388222928502	21.09662403964063
40	19.84233839685678	19.76275682574639	36.339748241947994	24.055156535448834
41	14.59996496408819	20.29380114617483	34.73960809830076	30.366625791436224
42	20.233739583072648	19.775269651392676	33.708050752020824	26.28294001351385
43	20.2847919117095	19.33531870166921	35.47736429840587	24.90252508821542
44	18.87334517880828	23.613203533621963	33.34718086038189	24.166270427187868
45	18.43689782026577	23.00758277234165	32.982807377562004	25.572712029830573
46	23.134713080907932	23.106183838434397	32.248054255612004	21.511048825045673
47	17.245176305713354	20.505017643084162	36.904326935108486	25.345479116093994
48	18.01396431342126	19.856853274606472	33.941289822067624	28.18789258990465
49	20.502515077954904	17.64808929152381	37.05097725168297	24.79841837883831
50	18.77074000850872	18.88285492629946	36.2241297329763	26.122275332215523
51	16.83225305938587	18.945919567556746	31.302585149778523	32.91924222327886
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	610.0
1	535.5
2	461.0
3	1655.0
4	2849.0
5	2525.5
6	2202.0
7	1791.0
8	1380.0
9	1414.5
10	1449.0
11	1446.0
12	1443.0
13	1410.0
14	1377.0
15	1313.5
16	1250.0
17	1237.0
18	1224.0
19	1296.0
20	1368.0
21	1408.5
22	1449.0
23	1575.5
24	1702.0
25	1665.5
26	1779.5
27	1930.0
28	2190.0
29	2450.0
30	2605.0
31	2760.0
32	3229.0
33	3698.0
34	4244.5
35	4791.0
36	5267.0
37	5743.0
38	6350.5
39	6958.0
40	8336.5
41	9715.0
42	12295.5
43	14876.0
44	17701.0
45	20526.0
46	24401.0
47	28276.0
48	27872.0
49	27468.0
50	24787.0
51	22106.0
52	17438.0
53	12770.0
54	9961.5
55	7153.0
56	5420.5
57	3688.0
58	2843.0
59	1998.0
60	1540.0
61	1082.0
62	845.0
63	608.0
64	462.5
65	317.0
66	250.5
67	184.0
68	158.0
69	132.0
70	93.5
71	55.0
72	53.5
73	52.0
74	38.0
75	21.0
76	18.0
77	14.0
78	10.0
79	7.5
80	5.0
81	4.0
82	3.0
83	2.0
84	1.0
85	1.0
86	1.0
87	1.0
88	1.0
89	2.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	199795.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.35271065355214
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.93393125374085	41.32405263674471
2	9.4325369746377	9.876377579242792
3	3.5863552977552593	5.632662636126448
4	1.820185218507538	3.811665203215909
5	1.1696965627704112	3.0618392851586917
6	0.8100871194927186	2.544615394258309
7	0.601321567844575	2.2036569835775155
8	0.47546349699793605	1.9913442307727205
9	0.4405029389796248	2.0755370615795665
>10	2.676979306863107	21.71234533630147
>50	0.02996618626997489	1.0369841233395942
>100	0.013984220259321615	1.3214618656980104
>500	0.007990983005326637	2.5927217768621826
>1k	9.988728756658296E-4	0.8147358871220817
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1558	0.7797992942766335	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT	721	0.3608698916389299	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	718	0.35936835256137545	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	650	0.3253334668034736	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTC	627	0.3138216672088891	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCG	592	0.2963037113040867	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	554	0.2772842163217298	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	550	0.2752821642183238	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGC	546	0.2732801121149178	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	322	0.1611651943241823	No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	250	0.12512825646287445	No Hit
ACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	207	0.10360619635126005	No Hit
GAACTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCT	200	0.10010260517029955	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5495633023849446	0.0
2	0.0	0.0	0.0	1.771816111514302	0.0
3	0.0	0.0	0.0	2.3083660752271076	0.0
4	0.0	0.0	0.0	3.210290547811507	0.0
5	0.0	0.0	0.0	5.4000350359118094	0.0
6	0.0	0.0	0.0	6.9140869391125905	0.0
7	0.0	0.0	0.0	8.168873094922295	0.0
8	0.0	0.0	0.0	12.127430616381792	0.0
9	0.0	0.0	0.0	13.673014840211216	0.0
10	0.0	0.0	0.0	17.088515728621836	0.0
11	0.0	0.0	0.0	19.518005956105007	0.0
12	0.0	0.0	0.0	22.18523987086764	0.0
13	0.0	0.0	0.0	23.06764433544383	0.0
14	0.0	0.0	0.0	23.41600140143647	0.0
15	0.0	0.0	0.0	24.01311344127731	0.0
16	0.0	0.0	0.0	25.325458595059935	0.0
17	0.0	0.0	0.0	26.558722690758028	0.0
18	0.0	0.0	0.0	28.913135964363473	0.0
19	0.0	0.0	0.0	29.6744162766836	0.0
20	0.0	0.0	0.0	30.649415651042318	0.0
21	0.0	0.0	0.0	31.487774969343576	0.0
22	0.0	0.0	0.0	32.29960709727471	0.0
23	0.0	0.0	0.0	33.07289972221527	0.0
24	0.0	0.0	0.0	33.670011762056106	0.0
25	0.0	0.0	0.0	34.178032483295375	0.0
26	0.0	0.0	0.0	34.68355063940539	0.0
27	0.0	0.0	0.0	35.18506469130859	0.0
28	0.0	0.0	0.0	35.655546935608996	0.0
29	0.0	0.0	0.0	36.11201481518557	0.0
30	0.0	0.0	0.0	36.73265096724142	0.0
31	0.0	0.0	0.0	37.256688105307944	0.0
32	0.0	0.0	0.0	37.72917240171176	0.0
33	0.0	0.0	0.0	38.18864335944343	0.0
34	0.0	0.0	0.0	38.639105082709776	0.0
35	0.0	0.0	0.0	39.093070397157085	0.0
36	0.0	0.0	0.0	39.52100903426012	0.0
37	0.0	0.0	0.0	39.96045947095773	0.0
38	0.0	0.0	0.0	40.3838934908281	0.0
39	0.0	0.0	0.0	40.80782802372432	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTAATC	25	3.879201E-5	45.000004	8
TTTACGG	25	3.879201E-5	45.000004	2
TTAGCGG	35	1.2051896E-7	45.000004	2
GTCCTGC	30	2.156472E-6	45.000004	14
CTCCACT	30	2.156472E-6	45.000004	5
TAAGTAG	25	3.879201E-5	45.000004	1
CAGGGCC	25	3.879201E-5	45.000004	5
AACGGGA	30	2.156472E-6	45.000004	4
AACATTG	25	3.879201E-5	45.000004	15
TCCACTC	30	2.156472E-6	45.000004	6
CGGGATT	60	0.0	45.000004	6
TAGGGTC	30	2.156472E-6	45.000004	5
CTCTGTG	30	2.156472E-6	45.000004	18
TCATAAT	25	3.879201E-5	45.000004	44
GGCGATC	25	3.879201E-5	45.000004	8
TGGGACC	50	2.1827873E-11	45.000004	6
CTCTAGT	25	3.879201E-5	45.000004	10
AGTACGG	30	2.156472E-6	45.000004	2
TGGTACC	30	2.156472E-6	45.000004	15
TCAGTAG	25	3.879201E-5	45.000004	1
>>END_MODULE