##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549488_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	420940
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40212619375683	31.0	31.0	34.0	30.0	34.0
2	31.534411079963892	31.0	31.0	34.0	30.0	34.0
3	31.143319713023235	31.0	31.0	34.0	28.0	34.0
4	35.2323062669264	37.0	35.0	37.0	33.0	37.0
5	35.09089894046657	35.0	35.0	37.0	32.0	37.0
6	35.115900128284316	36.0	35.0	37.0	32.0	37.0
7	32.06651779350977	35.0	35.0	37.0	16.0	37.0
8	33.59298237278472	37.0	35.0	37.0	19.0	37.0
9	36.48538984178268	39.0	35.0	39.0	32.0	39.0
10	36.24686416116311	38.0	35.0	39.0	32.0	39.0
11	36.32541217275621	38.0	35.0	39.0	32.0	39.0
12	36.423487908015396	38.0	35.0	39.0	32.0	39.0
13	32.860381527058486	37.0	34.0	39.0	11.0	39.0
14	35.30836223689837	38.0	34.0	40.0	18.0	41.0
15	36.608462013588635	39.0	35.0	40.0	30.0	41.0
16	37.188528056255045	39.0	36.0	40.0	32.0	41.0
17	37.32384425333777	39.0	36.0	40.0	32.0	41.0
18	37.281638713355825	39.0	36.0	40.0	32.0	41.0
19	37.27882358530907	39.0	36.0	40.0	32.0	41.0
20	37.20429990022331	39.0	35.0	40.0	32.0	41.0
21	37.13216372879745	39.0	35.0	40.0	32.0	41.0
22	37.14393025134223	39.0	35.0	40.0	32.0	41.0
23	37.01753694113175	39.0	35.0	40.0	32.0	41.0
24	36.943875611726135	39.0	35.0	40.0	32.0	41.0
25	36.74942747184872	38.0	35.0	40.0	31.0	41.0
26	36.63945217845774	38.0	35.0	40.0	31.0	41.0
27	36.63164346462679	38.0	35.0	40.0	31.0	41.0
28	36.456601891005846	38.0	35.0	40.0	30.0	41.0
29	36.400688934289924	38.0	35.0	40.0	30.0	41.0
30	36.25220934099872	38.0	35.0	40.0	30.0	41.0
31	36.15072932009313	38.0	35.0	40.0	30.0	41.0
32	35.9645864018625	38.0	35.0	40.0	30.0	41.0
33	35.78922886872238	38.0	35.0	40.0	29.0	41.0
34	35.605506723048414	38.0	35.0	40.0	27.0	41.0
35	35.53089038817884	38.0	35.0	40.0	27.0	41.0
36	35.28989404665748	38.0	34.0	40.0	26.0	41.0
37	35.245607449992875	38.0	34.0	40.0	25.0	41.0
38	35.092215042523875	38.0	34.0	40.0	25.0	41.0
39	34.95681569819927	38.0	34.0	40.0	24.0	41.0
40	34.86232717251865	38.0	34.0	40.0	24.0	41.0
41	34.80414548391695	38.0	34.0	40.0	24.0	41.0
42	34.73842115265833	38.0	34.0	40.0	24.0	41.0
43	34.684083242267306	38.0	34.0	40.0	23.0	41.0
44	34.51358388368889	38.0	33.0	40.0	23.0	41.0
45	34.51720672779969	38.0	33.0	40.0	23.0	41.0
46	34.39053784387323	38.0	33.0	40.0	23.0	41.0
47	34.200244690454696	38.0	33.0	40.0	22.0	41.0
48	34.108716206585264	37.0	33.0	40.0	22.0	41.0
49	33.97950301705706	37.0	33.0	40.0	21.0	41.0
50	33.74639616097306	37.0	33.0	40.0	20.0	41.0
51	31.72818453936428	35.0	29.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	7.0
10	12.0
11	17.0
12	10.0
13	10.0
14	6.0
15	17.0
16	47.0
17	89.0
18	177.0
19	404.0
20	698.0
21	1205.0
22	1873.0
23	2791.0
24	3959.0
25	5229.0
26	6740.0
27	7518.0
28	8086.0
29	9120.0
30	11108.0
31	13969.0
32	18203.0
33	24405.0
34	33150.0
35	41204.0
36	47583.0
37	63408.0
38	68968.0
39	50922.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.7614386848482	23.70955480591058	27.481113697914196	16.04789281132703
2	31.3802442153276	26.65130422387989	28.672257328835464	13.296194231957049
3	29.992635530004275	26.12106238418777	30.927685655912956	12.958616429894999
4	25.623129187057536	27.839597092222167	31.341996484059486	15.195277236660806
5	21.747517460920797	32.36138166959662	29.959614196797645	15.931486672684944
6	20.210006176652254	41.137454269016956	28.28455361809284	10.367985936237943
7	73.27196275003564	13.994631063809567	9.840832422673065	2.8925737634817312
8	78.16862260654726	5.707939373782487	10.845726231766998	5.2777117879032645
9	71.09350501259087	8.470803439920179	14.562645507673302	5.873046039815651
10	29.20463724046182	36.4512757162541	20.206442723428516	14.13764431985556
11	24.746757257566397	26.828764194422007	32.225020192901596	16.199458355109993
12	24.948686273578183	22.418634484724663	34.23623319237896	18.396446049318193
13	19.73202831757495	32.43787713213285	32.622701572670685	15.207392977621515
14	16.75440680382002	31.160260369648878	32.3385755689647	19.7467572575664
15	15.076733026084476	26.79621798831187	41.67387276096356	16.453176224640092
16	16.753931676723525	25.984938471041	37.46139592340951	19.79973392882596
17	16.329168052454033	26.069273530669456	33.25675868294769	24.344799733928827
18	17.987836746329645	25.965458260084574	36.301373117308884	19.745331876276904
19	20.974485674918043	27.86477882833658	32.56259799496365	18.59813750178173
20	21.995296241744665	26.13008979902124	32.02309117688982	19.851522782344276
21	19.69330545921034	28.762056350073646	32.83294531287119	18.711692877844825
22	17.629353352021663	26.698816933529717	30.889913051741342	24.781916662707275
23	16.897182496317765	29.478072884496605	31.470280800114033	22.1544638190716
24	17.13545873521167	26.41896707369221	34.94108424003421	21.50448995106191
25	17.22311968451561	29.42533377678529	31.710695110942176	21.640851427756925
26	15.705326174751747	29.171853470803438	33.11754644367368	22.00527391077113
27	18.666318240129236	28.45322373734974	31.268826911198744	21.61163111132228
28	15.925785147526964	28.897230009027414	34.75887299852711	20.418111844918517
29	17.42932484439588	27.410557324084195	31.781251484772177	23.378866346747753
30	18.202119066850383	27.9020763054117	31.874613959234093	22.021190668503827
31	18.80624317004799	28.57200551147432	29.05901078538509	23.5627405330926
32	18.782011688126573	29.585451608305224	29.42557134033354	22.206965363234666
33	17.573763481731362	28.455361809283986	28.9720625267259	24.998812182258753
34	16.474794507530767	27.897562597994963	31.720435216420395	23.90720767805388
35	16.173326364802584	26.622083907445244	29.06732550957381	28.137264218178366
36	17.39107711312776	30.44566921651542	30.164156411840164	21.999097258516652
37	16.33296906922602	28.88083812419822	30.55471088516178	24.231481921413977
38	16.049555756164775	29.40585356582886	29.084667648596003	25.45992302941037
39	17.935810329263077	28.364137406756306	28.482919180880884	25.21713308309973
40	18.204257138784623	27.209578562265406	31.086853233239893	23.499311065710078
41	15.618140352544305	28.062906827576377	29.589727752173705	26.72922506770561
42	18.82524825390792	27.83650876609493	29.909963415213568	23.42827956478358
43	18.417826768660618	26.343421865348983	31.577897087470895	23.660854278519505
44	17.73554425808904	28.223499786192807	30.447569724901413	23.593386230816744
45	16.810946928303323	27.088658716206588	30.21689551955148	25.88349883593861
46	19.314391599752934	28.374115075782775	29.175892051123675	23.13560127334062
47	16.874138832137596	26.84681902408894	31.69644129804723	24.582600845726233
48	17.299852710600085	26.107283698389317	30.74333634247161	25.849527248538983
49	18.441345559937282	25.896327267544066	31.601653442295813	24.060673730222835
50	16.930916520169145	25.31904784529862	32.48538984178268	25.264645792749562
51	17.042571387846248	24.525585594146435	29.387086045517176	29.044756972490145
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	418.0
1	581.0
2	744.0
3	3241.5
4	5739.0
5	5087.0
6	4435.0
7	3451.5
8	2468.0
9	2402.5
10	2337.0
11	2284.5
12	2232.0
13	2223.5
14	2215.0
15	2108.0
16	2001.0
17	2088.0
18	2175.0
19	2182.5
20	2190.0
21	2321.0
22	2452.0
23	2752.0
24	3052.0
25	3579.0
26	4897.0
27	5688.0
28	6693.0
29	7698.0
30	8900.0
31	10102.0
32	11501.5
33	12901.0
34	13939.0
35	14977.0
36	16032.0
37	17087.0
38	18572.5
39	20058.0
40	22506.5
41	24955.0
42	27552.5
43	30150.0
44	33327.0
45	36504.0
46	41720.5
47	46937.0
48	47176.5
49	47416.0
50	43346.5
51	39277.0
52	32380.0
53	25483.0
54	21110.5
55	16738.0
56	13507.0
57	10276.0
58	8664.5
59	7053.0
60	5922.5
61	4792.0
62	3747.0
63	2702.0
64	2047.5
65	1393.0
66	1085.5
67	778.0
68	626.0
69	474.0
70	421.5
71	369.0
72	305.5
73	242.0
74	204.0
75	121.5
76	77.0
77	55.0
78	33.0
79	29.5
80	26.0
81	18.0
82	10.0
83	10.5
84	11.0
85	7.0
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	420940.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.500004825692685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.33944083095862	41.65321026493086
2	9.298569181077582	9.763498537572206
3	3.3722847357973444	5.311348947089111
4	1.7416070046867824	3.65737504602065
5	1.0542277838965968	2.7673481870975314
6	0.7463915394740874	2.351133565454747
7	0.5744052597753154	2.1109395237075126
8	0.49564232405713177	2.0816979523853565
9	0.4262667940170125	2.0141107868633137
>10	2.898978836009646	23.928026616825466
>50	0.03803365323308412	1.3269213117236096
>100	0.011056294544395497	1.2800205059451575
>500	0.0022112589088790994	0.7260337605823521
>1k	8.845035635516397E-4	1.0283349938021225
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3133	0.7442865966646078	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1296	0.30788235853090706	No Hit
GCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	722	0.1715208818358911	No Hit
CTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGCT	669	0.1589300137786858	No Hit
CCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTCTGC	613	0.14562645507673302	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCGTCTTC	608	0.14443863733548723	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGCCG	515	0.12234522734831568	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTCACCATCGTATGC	463	0.10999192283935952	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	440	0.10452796122962893	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3756354824915665E-4	0.0	0.0	0.23756354824915665	0.0
2	2.3756354824915665E-4	0.0	0.0	0.8179312966218464	0.0
3	2.3756354824915665E-4	0.0	0.0	1.1167862403192854	0.0
4	2.3756354824915665E-4	0.0	0.0	1.615194564546016	0.0
5	2.3756354824915665E-4	0.0	0.0	2.836983893191429	0.0
6	2.3756354824915665E-4	0.0	0.0	3.801491899083005	0.0
7	2.3756354824915665E-4	0.0	0.0	4.512994726089229	0.0
8	2.3756354824915665E-4	0.0	0.0	6.7325509573810995	0.0
9	2.3756354824915665E-4	0.0	0.0	7.6089228868722385	0.0
10	2.3756354824915665E-4	0.0	0.0	9.464056635149902	0.0
11	2.3756354824915665E-4	0.0	0.0	11.001092792321947	0.0
12	2.3756354824915665E-4	0.0	0.0	12.547869054972205	0.0
13	2.3756354824915665E-4	0.0	0.0	13.122535278186914	0.0
14	2.3756354824915665E-4	0.0	0.0	13.382192236423244	0.0
15	2.3756354824915665E-4	0.0	0.0	13.769895947165868	0.0
16	2.3756354824915665E-4	0.0	0.0	14.562170380576804	0.0
17	2.3756354824915665E-4	0.0	0.0	15.459447902313869	0.0
18	4.751270964983133E-4	0.0	0.0	16.827101249584263	0.0
19	4.751270964983133E-4	0.0	0.0	17.348790801539412	0.0
20	4.751270964983133E-4	0.0	0.0	18.064094645317624	0.0
21	4.751270964983133E-4	0.0	0.0	18.778923361999336	0.0
22	4.751270964983133E-4	0.0	0.0	19.59471658668694	0.0
23	4.751270964983133E-4	0.0	0.0	20.37440015204067	0.0
24	4.751270964983133E-4	0.0	0.0	20.95548059105811	0.0
25	4.751270964983133E-4	0.0	0.0	21.43654677626265	0.0
26	4.751270964983133E-4	0.0	0.0	21.897182496317765	0.0
27	4.751270964983133E-4	0.0	0.0	22.3796740628118	0.0
28	4.751270964983133E-4	0.0	0.0	22.89304889057823	0.0
29	7.126906447474699E-4	0.0	0.0	23.417589205112368	0.0
30	7.126906447474699E-4	0.0	0.0	24.03406661281893	0.0
31	7.126906447474699E-4	0.0	0.0	24.643892241174512	0.0
32	7.126906447474699E-4	0.0	0.0	25.19694018149855	0.0
33	7.126906447474699E-4	0.0	0.0	25.721955623129187	0.0
34	7.126906447474699E-4	0.0	0.0	26.270964983132988	0.0
35	7.126906447474699E-4	0.0	0.0	26.8451560792512	0.0
36	9.502541929966266E-4	0.0	0.0	27.421010120207157	0.0
37	9.502541929966266E-4	0.0	0.0	27.974058060531192	0.0
38	9.502541929966266E-4	0.0	0.0	28.492184159262603	0.0
39	9.502541929966266E-4	0.0	0.0	29.043806718297144	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTTA	125	0.0	45.000004	1
AACGGGA	75	0.0	45.000004	4
ACGGTTA	35	1.2094824E-7	45.000004	34
CGAACGG	20	7.028187E-4	45.0	2
TAGCGCG	25	3.8865786E-5	45.0	1
CGATTTA	25	3.8865786E-5	45.0	10
TCGATGG	20	7.028187E-4	45.0	2
GTTCGGG	40	6.7975634E-9	45.0	3
TTAAGCG	25	3.8865786E-5	45.0	1
GTCGTTA	20	7.028187E-4	45.0	14
TATAGCG	20	7.028187E-4	45.0	1
AATAGCG	20	7.028187E-4	45.0	1
TCGACTG	20	7.028187E-4	45.0	1
AGTACGG	40	6.7975634E-9	45.0	2
GCGATAA	30	2.1622127E-6	44.999996	9
TCCGGGA	30	2.1622127E-6	44.999996	4
TAACGGG	85	0.0	42.35294	3
CGGTTTT	480	0.0	41.718746	1
TAATACG	65	0.0	41.53846	12
CGTTTTT	1485	0.0	41.363636	1
>>END_MODULE