FastQCFastQC Report
Sat 18 Jun 2016
SRR3549486_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549486_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480529
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28430.5916396304905636No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10620.22100643249418872No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6910.14379985391100225No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6530.13589190246582414No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA5250.1092545923346978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTT302.1628548E-645.00000427
ACGATGG207.029183E-445.0000042
TCTACGG302.1628548E-645.0000042
GCTACCG207.029183E-445.0000041
CGCCGTT302.1628548E-645.00000426
TCGACGT302.1628548E-645.00000426
GTACGAG207.029183E-445.0000041
CTAGCGG207.029183E-445.0000042
GTTAACG253.887406E-545.01
TCACTCG253.887406E-545.021
CGTGTAC351.2099554E-745.018
AGTACGG253.887406E-545.02
GGGCGAA1000.042.757
ATGGGCG950.042.631585
TAACGGG700.041.7857133
TACGAAA700.041.78571320
CATACGA700.041.78571318
TGGGCGA2000.041.6256
GCTACGA650.041.5384610
CGGTTTT4400.041.4204561
Produced by FastQC (version 0.11.5)