##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549486_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	480529
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60372630996256	31.0	31.0	34.0	30.0	34.0
2	31.759013503867614	31.0	31.0	34.0	30.0	34.0
3	31.427381073774942	31.0	31.0	34.0	28.0	34.0
4	35.43723271644375	37.0	35.0	37.0	33.0	37.0
5	35.279872806844125	37.0	35.0	37.0	33.0	37.0
6	35.32444659947683	37.0	35.0	37.0	33.0	37.0
7	32.10053295430661	37.0	35.0	37.0	10.0	37.0
8	33.689073916454575	37.0	35.0	37.0	19.0	37.0
9	36.60798411750383	39.0	35.0	39.0	32.0	39.0
10	36.45206012540346	38.0	35.0	39.0	32.0	39.0
11	36.582984585737805	38.0	35.0	39.0	32.0	39.0
12	36.61867858131351	38.0	35.0	39.0	32.0	39.0
13	32.94041150482073	38.0	35.0	39.0	10.0	39.0
14	35.49590139200756	39.0	35.0	40.0	18.0	41.0
15	36.845665922348076	39.0	35.0	40.0	31.0	41.0
16	37.408932655469286	39.0	36.0	40.0	32.0	41.0
17	37.52731260756375	39.0	36.0	40.0	33.0	41.0
18	37.50802344915707	39.0	36.0	40.0	32.0	41.0
19	37.54072074734303	39.0	36.0	40.0	32.0	41.0
20	37.5038155865723	39.0	36.0	40.0	33.0	41.0
21	37.4340778600251	39.0	36.0	40.0	32.0	41.0
22	37.4282696777926	39.0	35.0	40.0	32.0	41.0
23	37.33230252492565	39.0	35.0	40.0	32.0	41.0
24	37.25263199515534	39.0	35.0	40.0	32.0	41.0
25	37.057311837579	39.0	35.0	40.0	32.0	41.0
26	36.952136083358134	39.0	35.0	40.0	32.0	41.0
27	36.962306125124606	39.0	35.0	40.0	32.0	41.0
28	36.81042559346054	39.0	35.0	40.0	31.0	41.0
29	36.782042290891916	39.0	35.0	40.0	31.0	41.0
30	36.63806138651361	39.0	35.0	40.0	31.0	41.0
31	36.532319589452456	38.0	35.0	40.0	30.0	41.0
32	36.371873497749355	38.0	35.0	40.0	30.0	41.0
33	36.197328361035446	38.0	35.0	40.0	30.0	41.0
34	36.05826495383213	38.0	35.0	40.0	29.0	41.0
35	35.92188400700062	38.0	35.0	40.0	29.0	41.0
36	35.771435230756104	38.0	35.0	40.0	28.0	41.0
37	35.66722507902749	38.0	35.0	40.0	27.0	41.0
38	35.52400999731546	38.0	35.0	40.0	27.0	41.0
39	35.422765327378784	38.0	34.0	40.0	26.0	41.0
40	35.39208871889105	38.0	34.0	40.0	26.0	41.0
41	35.38355021237012	38.0	34.0	40.0	26.0	41.0
42	35.360313321360415	38.0	34.0	40.0	26.0	41.0
43	35.32576181666455	38.0	34.0	40.0	26.0	41.0
44	35.10871976509222	38.0	34.0	40.0	24.0	41.0
45	35.068243539932034	38.0	34.0	40.0	24.0	41.0
46	34.97718139800095	38.0	34.0	40.0	24.0	41.0
47	34.82063309394438	38.0	34.0	40.0	24.0	41.0
48	34.70051339253198	38.0	34.0	40.0	23.0	41.0
49	34.647534279929	38.0	33.0	40.0	24.0	41.0
50	34.46287528952467	38.0	33.0	40.0	23.0	41.0
51	32.47359680685245	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	13.0
10	26.0
11	8.0
12	6.0
13	6.0
14	4.0
15	15.0
16	36.0
17	76.0
18	153.0
19	343.0
20	623.0
21	1103.0
22	1649.0
23	2480.0
24	3628.0
25	5179.0
26	6551.0
27	7480.0
28	8192.0
29	9386.0
30	11204.0
31	14594.0
32	18893.0
33	25817.0
34	35883.0
35	44415.0
36	52113.0
37	73791.0
38	84632.0
39	72218.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.25605738675501	24.036426521604316	27.981245668835804	14.726270422804866
2	32.381396336121234	26.739905395928236	28.082592309725324	12.796105958225207
3	30.974613394821127	25.627589593968313	30.38796825998015	13.009828751230415
4	26.644593770615298	27.30303478041908	30.950057124543996	15.102314324421625
5	22.626105812552417	33.13369224334015	28.79493225174755	15.445269692359878
6	21.428467376578727	41.77812369284684	26.88432956179544	9.909079368778992
7	75.7802338672588	14.067829413001087	7.959769337542583	2.1921673821975367
8	82.47410666161667	5.565741089507605	8.648801633200078	3.3113506156756407
9	74.03673867758242	9.381535765791451	11.679003764601095	4.902721792025039
10	27.71487256752454	42.27028961831648	17.623077899564855	12.391759914594125
11	27.268489518842777	27.320515515192632	29.751378168643306	15.659616797321284
12	26.21943732844428	24.10301979693213	32.22511024308626	17.452432631537327
13	20.109920525087976	33.91949289220838	30.57546995082503	15.395116631878617
14	17.95313082040834	31.262837414599325	32.77325614062835	18.01077562436398
15	17.493637220646413	27.423943195936147	38.25617184394699	16.82624773947046
16	19.797764546988837	27.11178721783701	35.12316634375865	17.967281891415503
17	18.645284675846828	26.274792988560524	32.29128731044328	22.788635025149368
18	19.31600382078917	26.65936915357866	34.97000181050468	19.054625215127494
19	22.608833181764265	27.665551922984875	31.456166017035393	18.269448878215467
20	22.67126437738409	26.933858310320502	31.683415569091565	18.711461743203845
21	21.34480957444816	28.637189430814793	32.23072905069205	17.787271944045
22	19.49726239207207	25.529364512859786	31.231413712804013	23.74195938226413
23	17.940644581284378	29.115620493248066	31.63867321223069	21.30506171323687
24	18.248638479675524	27.553591978839986	33.81044640385908	20.38732313762541
25	18.30045637203998	29.34682402102683	31.523383604319406	20.829336002613786
26	17.20249974507262	30.023161973574958	31.60558467855218	21.168753602800248
27	18.79636816924681	28.899192348432667	31.55189385031913	20.7525456320014
28	17.28740617111558	28.29360975092034	34.73879828272592	19.680185795238163
29	18.519797972650977	27.56691063390555	32.595327233111846	21.317964160331634
30	18.616566325861704	28.58911741018752	32.84754926341594	19.946767000534827
31	21.55832426346797	28.305263574102707	29.99798139134163	20.138430771087698
32	21.278424403105745	28.313379629533287	31.3799999583792	19.02819600898177
33	19.871017149849436	28.006842459039934	30.400038291133313	21.722102099977317
34	18.955983926048166	27.143002815646923	33.146386586449516	20.75462667185539
35	19.694961178201524	26.612961964834586	32.012427970008055	21.679648886955835
36	21.813251645582266	27.65389809980251	31.373548734831818	19.159301519783405
37	19.532015757633776	30.147191948873015	30.823321797435742	19.49747049605747
38	19.85083106326569	29.979876344611878	30.296610610389802	19.872681981732633
39	19.612135792012552	28.071770902484555	31.175641844716967	21.140451460785926
40	20.681582172980196	26.48872388555113	33.277075889280354	19.55261805218832
41	17.998705593210815	26.853738275941723	31.695901808215527	23.45165432263193
42	19.580087778261042	27.641828068649342	30.68347591924733	22.094608233842287
43	19.950720976257415	25.984487928928328	31.06659535636767	22.998195738446586
44	19.395707647197153	27.702594432385975	31.3444141768759	21.55728374354097
45	18.306699491601965	29.034043730971494	31.051195661448112	21.608061115978433
46	20.157160129773647	29.05443792154064	30.794603447450626	19.993798501235098
47	19.32474418817595	26.71014652601612	32.623837479111565	21.341271806696373
48	19.106859315462753	26.78173429699352	32.181408406152386	21.92999798139134
49	19.36823792112443	26.022154750285626	32.819871433357825	21.78973589523213
50	18.62926066897107	26.316621889625807	33.27000035377677	21.784117087626345
51	18.8356998224873	25.51937552156061	31.480514183327124	24.16441047262496
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	394.0
1	602.0
2	810.0
3	3205.0
4	5600.0
5	4888.5
6	4177.0
7	3282.5
8	2388.0
9	2302.5
10	2217.0
11	2278.5
12	2340.0
13	2320.0
14	2300.0
15	2218.5
16	2137.0
17	2088.0
18	2039.0
19	2275.0
20	2511.0
21	2656.0
22	2801.0
23	3240.5
24	3680.0
25	4774.0
26	6763.5
27	7659.0
28	9260.5
29	10862.0
30	12175.0
31	13488.0
32	15065.5
33	16643.0
34	17957.5
35	19272.0
36	20601.5
37	21931.0
38	24294.0
39	26657.0
40	29502.5
41	32348.0
42	34732.0
43	37116.0
44	39727.0
45	42338.0
46	45229.5
47	48121.0
48	47149.0
49	46177.0
50	42280.0
51	38383.0
52	32620.0
53	26857.0
54	22457.5
55	18058.0
56	15040.5
57	12023.0
58	9949.5
59	7876.0
60	6791.5
61	5707.0
62	4701.5
63	3696.0
64	2937.5
65	2179.0
66	1795.0
67	1411.0
68	1149.0
69	887.0
70	759.0
71	631.0
72	532.0
73	433.0
74	331.0
75	190.0
76	151.0
77	107.5
78	64.0
79	48.5
80	33.0
81	26.5
82	20.0
83	13.0
84	6.0
85	5.0
86	4.0
87	3.5
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	480529.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.228800048520675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.64610639075154	36.671324332749684
2	9.373118282013495	8.85362258344698
3	3.698655300949346	5.240491549708132
4	2.011575745161624	3.8001723460276935
5	1.2306587085305827	2.9061267036580793
6	0.821174243102815	2.326984447949889
7	0.6001730612459724	1.9841817452866208
8	0.48487413877452473	1.8320018999104557
9	0.4137191599815486	1.7585513534709452
>10	3.6611447933880235	30.481423627248773
>50	0.0407738447428171	1.255758949097749
>100	0.015880339529441262	1.7189404176409004
>500	0.001287595096981724	0.3788560896899578
>1k	8.583967313211493E-4	0.7915639541141174
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2843	0.5916396304905636	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1062	0.22100643249418872	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	691	0.14379985391100225	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	653	0.13589190246582414	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	525	0.1092545923346978	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.081039853994244E-4	0.0	0.0	0.11737064776527535	0.0
2	2.081039853994244E-4	0.0	0.0	0.4386832012219866	0.0
3	2.081039853994244E-4	0.0	0.0	0.6039177656291296	0.0
4	2.081039853994244E-4	0.0	0.0	0.8613423955682176	0.0
5	2.081039853994244E-4	0.0	0.0	1.5149970137078095	0.0
6	2.081039853994244E-4	0.0	0.0	2.0577322076295084	0.0
7	2.081039853994244E-4	0.0	0.0	2.452297363946817	0.0
8	2.081039853994244E-4	0.0	0.0	3.5606591901841513	0.0
9	2.081039853994244E-4	0.0	0.0	4.115672519244416	0.0
10	2.081039853994244E-4	0.0	0.0	5.33641049759744	0.0
11	2.081039853994244E-4	0.0	0.0	6.30513454963176	0.0
12	2.081039853994244E-4	0.0	0.0	7.283223281009055	0.0
13	2.081039853994244E-4	0.0	0.0	7.671961525735179	0.0
14	2.081039853994244E-4	0.0	0.0	7.838652818040118	0.0
15	2.081039853994244E-4	0.0	0.0	8.116055430577552	0.0
16	2.081039853994244E-4	0.0	0.0	8.664201328119635	0.0
17	2.081039853994244E-4	0.0	0.0	9.282062060770526	0.0
18	2.081039853994244E-4	0.0	0.0	10.189811645082814	0.0
19	2.081039853994244E-4	0.0	0.0	10.572306770246957	0.0
20	2.081039853994244E-4	0.0	0.0	11.043246089205855	0.0
21	2.081039853994244E-4	0.0	0.0	11.565795196543808	0.0
22	2.081039853994244E-4	0.0	0.0	12.107073662567712	0.0
23	2.081039853994244E-4	0.0	0.0	12.654803352138996	0.0
24	2.081039853994244E-4	0.0	0.0	13.081416522207817	0.0
25	2.081039853994244E-4	0.0	0.0	13.454338864043585	0.0
26	2.081039853994244E-4	0.0	0.0	13.799375271835832	0.0
27	2.081039853994244E-4	0.0	0.0	14.165430182153418	0.0
28	2.081039853994244E-4	0.0	0.0	14.550838763113152	0.0
29	2.081039853994244E-4	0.0	0.0	14.97453847738638	0.0
30	2.081039853994244E-4	0.0	0.0	15.46628819488522	0.0
31	2.081039853994244E-4	0.0	0.0	15.9076767479174	0.0
32	2.081039853994244E-4	0.0	0.0	16.346359949139387	0.0
33	2.081039853994244E-4	0.0	0.0	16.79357541376275	0.0
34	2.081039853994244E-4	0.0	0.0	17.222477727670963	0.0
35	2.081039853994244E-4	0.0	0.0	17.69695481438165	0.0
36	2.081039853994244E-4	0.0	0.0	18.154367374289585	0.0
37	2.081039853994244E-4	0.0	0.0	18.606161126591736	0.0
38	2.081039853994244E-4	0.0	0.0	19.079389589390026	0.0
39	2.081039853994244E-4	0.0	0.0	19.547207348567934	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTT	30	2.1628548E-6	45.000004	27
ACGATGG	20	7.029183E-4	45.000004	2
TCTACGG	30	2.1628548E-6	45.000004	2
GCTACCG	20	7.029183E-4	45.000004	1
CGCCGTT	30	2.1628548E-6	45.000004	26
TCGACGT	30	2.1628548E-6	45.000004	26
GTACGAG	20	7.029183E-4	45.000004	1
CTAGCGG	20	7.029183E-4	45.000004	2
GTTAACG	25	3.887406E-5	45.0	1
TCACTCG	25	3.887406E-5	45.0	21
CGTGTAC	35	1.2099554E-7	45.0	18
AGTACGG	25	3.887406E-5	45.0	2
GGGCGAA	100	0.0	42.75	7
ATGGGCG	95	0.0	42.63158	5
TAACGGG	70	0.0	41.785713	3
TACGAAA	70	0.0	41.785713	20
CATACGA	70	0.0	41.785713	18
TGGGCGA	200	0.0	41.625	6
GCTACGA	65	0.0	41.53846	10
CGGTTTT	440	0.0	41.420456	1
>>END_MODULE