FastQCFastQC Report
Sat 18 Jun 2016
SRR3549482_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549482_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences551765
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25880.4690402617056174No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10620.19247324495029586No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGC8340.15115130535644705No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTC8140.14752657381312695No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCG7970.1444455520013049No Hit
CTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGCT5760.10439226844761809No Hit
GCTGTCTCTTATACACATCTGACGCGAAACCCATCGTATGCCGTCTTCTGC5570.10094877348146403No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG253.888159E-545.00000426
GCGCATG253.888159E-545.0000041
CGTACAT253.888159E-545.00000435
ATAACGG253.888159E-545.0000042
TAGCGCG207.030095E-445.01
AATGCGG453.8380676E-1045.02
GCGGGTC406.8048394E-945.05
CAGTCGA207.030095E-445.031
CTCACGA302.1634423E-644.99999624
GGTCGTT302.1634423E-644.9999968
CGGTTTA2050.043.902441
TACGGGA1850.042.5675664
AGGGCGC700.041.7857176
CACGGGC650.041.538464
TATGCGG603.6379788E-1241.2499962
CGGTTTT5700.041.052631
TACGAAT501.0786607E-940.50000412
CGACCTC451.9252184E-840.012
ACGATAG451.9252184E-840.01
TTAAGCG451.9252184E-840.01