FastQCFastQC Report
Sat 18 Jun 2016
SRR3549480_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549480_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences482319
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45230.9377611083121338No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGC37620.7799817133473904No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCG32930.6827431637567668No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTC30320.6286295999120913No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18480.38314891181977073No Hit
GCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGC10850.2249548535305472No Hit
GAACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCT8540.17706123955307587No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTC8210.1702192946991514No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCT7950.16482867148090785No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5460.11320308758311406No Hit
CTGTCTCTTATACACATCTGACGCAGGATCGTTCGTATGCCGTCTTCTGCT5110.10594647940470933Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGATCGTTCGTA4830.10014119286198553No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTG253.8874274E-545.023
GCGCGAC253.8874274E-545.09
CGGCAAC253.8874274E-545.09
CGCGACC253.8874274E-545.010
ACGATTG207.029212E-445.01
TCGTGAG207.029212E-445.01
GAAACGC253.8874274E-545.012
GCGGCTA207.029212E-445.024
CGATGAT207.029212E-445.010
GGCGCGA253.8874274E-545.08
CGATAGA253.8874274E-545.010
CGGTTTT7300.043.4589041
GGACCGA2100.042.8571438
AGGGCCG950.042.631586
CGGTTTA1800.042.51
AACGGGC603.6379788E-1241.2500044
CGTTTTT20250.041.111111
GTAGCGG1050.040.7142872
CGGGAGC501.0786607E-940.56
ACGGGAC900.040.05