FastQCFastQC Report
Sat 18 Jun 2016
SRR3549479_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549479_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371841
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21690.583313835752378No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9360.2517204934367108No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA5440.14629909020253282No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5140.1382311256693049No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4620.12424665381170984No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4330.11644762142958953No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3920.10542140323417805No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACCA253.8856975E-545.00000423
CGACGGT253.8856975E-545.00000428
CGGTTTA650.045.0000041
TACGGGT302.1615251E-645.0000044
CGGTCTA253.8856975E-545.00000431
ATAACGC302.1615251E-645.00000411
GGCGAGC253.8856975E-545.0000049
GTAACGG351.2089549E-745.0000042
AGTAACG253.8856975E-545.0000041
TCGCCGT207.0271234E-445.025
ATGGGTA800.045.05
ACACGCG453.8380676E-1045.036
GCACGTA207.0271234E-445.037
TCGGGCG207.0271234E-445.05
ACGGGTC207.0271234E-445.05
GCTCTCG207.0271234E-445.017
GAGCGCT207.0271234E-445.036
CATACGG207.0271234E-445.02
TTACGCT207.0271234E-445.014
CACGTAA207.0271234E-445.038