##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549479_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	371841
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.622201424802537	31.0	31.0	34.0	30.0	34.0
2	31.766860028883315	31.0	31.0	34.0	30.0	34.0
3	31.42550713880395	31.0	31.0	34.0	28.0	34.0
4	35.43951581455514	37.0	35.0	37.0	33.0	37.0
5	35.301419154961394	37.0	35.0	37.0	33.0	37.0
6	35.34312784227667	37.0	35.0	37.0	33.0	37.0
7	32.14800949868358	37.0	35.0	37.0	11.0	37.0
8	33.71373517175352	37.0	35.0	37.0	19.0	37.0
9	36.65500307927313	39.0	35.0	39.0	32.0	39.0
10	36.49513636204722	38.0	35.0	39.0	32.0	39.0
11	36.57632428914509	38.0	35.0	39.0	32.0	39.0
12	36.62897582568894	39.0	35.0	39.0	32.0	39.0
13	32.887290535470804	38.0	35.0	39.0	8.0	39.0
14	35.48621588259498	39.0	35.0	40.0	18.0	41.0
15	36.876788734970056	39.0	35.0	41.0	31.0	41.0
16	37.45182214978983	39.0	36.0	41.0	32.0	41.0
17	37.59434543259081	39.0	36.0	41.0	33.0	41.0
18	37.544106217442405	39.0	36.0	40.0	33.0	41.0
19	37.57020339338588	39.0	36.0	40.0	33.0	41.0
20	37.52016049870778	39.0	36.0	40.0	33.0	41.0
21	37.45154514967419	39.0	35.0	40.0	33.0	41.0
22	37.4380474450101	39.0	35.0	40.0	33.0	41.0
23	37.31627227766707	39.0	35.0	40.0	32.0	41.0
24	37.22571475442461	39.0	35.0	40.0	32.0	41.0
25	37.06133804502462	39.0	35.0	40.0	32.0	41.0
26	36.911951613727375	39.0	35.0	40.0	31.0	41.0
27	36.92264704537692	39.0	35.0	40.0	31.0	41.0
28	36.78553736677774	39.0	35.0	40.0	31.0	41.0
29	36.756124257411095	39.0	35.0	40.0	31.0	41.0
30	36.622174531587426	39.0	35.0	40.0	31.0	41.0
31	36.49612334304178	38.0	35.0	40.0	30.0	41.0
32	36.32927783649463	38.0	35.0	40.0	30.0	41.0
33	36.09990291549345	38.0	35.0	40.0	30.0	41.0
34	35.973416056863016	38.0	35.0	40.0	29.0	41.0
35	35.76225322113484	38.0	35.0	40.0	28.0	41.0
36	35.61491605282903	38.0	35.0	40.0	27.0	41.0
37	35.53335162071961	38.0	35.0	40.0	27.0	41.0
38	35.38965848306131	38.0	34.0	40.0	26.0	41.0
39	35.29126158761406	38.0	34.0	40.0	25.0	41.0
40	35.19385436248289	38.0	34.0	40.0	25.0	41.0
41	35.10106201306473	38.0	34.0	40.0	24.0	41.0
42	35.0809028590177	38.0	34.0	40.0	24.0	41.0
43	35.066329425749174	38.0	34.0	40.0	24.0	41.0
44	34.93036808743522	38.0	34.0	40.0	24.0	41.0
45	34.886709642024414	38.0	34.0	40.0	23.0	41.0
46	34.79100475740975	38.0	34.0	40.0	23.0	41.0
47	34.61400706215829	38.0	33.0	40.0	23.0	41.0
48	34.50231147183877	38.0	33.0	40.0	23.0	41.0
49	34.43458090958232	38.0	33.0	40.0	23.0	41.0
50	34.27200604559476	38.0	33.0	40.0	22.0	41.0
51	32.413125502566956	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	16.0
10	15.0
11	10.0
12	6.0
13	5.0
14	8.0
15	11.0
16	33.0
17	73.0
18	151.0
19	317.0
20	565.0
21	901.0
22	1461.0
23	2204.0
24	3076.0
25	4397.0
26	5466.0
27	6098.0
28	6490.0
29	7180.0
30	8920.0
31	11219.0
32	14381.0
33	19756.0
34	27609.0
35	34204.0
36	39159.0
37	56011.0
38	65300.0
39	56790.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.74269647510629	23.131661113217746	26.475025615787395	14.650616795888565
2	33.06359438577242	26.290269227976477	27.53596295190686	13.110173434344249
3	32.181496930139495	25.107505627405263	29.9515115331553	12.759485909299942
4	27.22749777458645	27.818072778418735	30.23496602042271	14.71946342657211
5	22.98374843010857	33.54202468259283	28.475074023574592	14.99915286372401
6	21.744778009955866	43.06491215331284	26.18780607840448	9.002503758326812
7	75.99995697085582	14.151478723432865	7.940759625754018	1.9078046799572936
8	82.31340815025777	5.907632563380584	8.666069637291207	3.112889649070436
9	74.60742629242068	9.264174741354504	11.444676622534901	4.683722343689911
10	29.783966803015264	39.0774551488405	18.194066818882266	12.944511229261972
11	27.635467847816674	29.00836647922096	28.529936182400544	14.826229490561825
12	26.88729860343534	24.891015245763644	31.721354019594393	16.50033213120662
13	20.56846878101124	35.809660580732086	28.410799239459877	15.211071398796797
14	17.677717088755678	33.20424590080169	31.256101398178256	17.861935612264382
15	18.064172589897296	28.148590392129968	37.935569235237644	15.851667782735094
16	19.249894444130693	27.12826181082775	35.594783791997116	18.027059953044446
17	18.854295249851415	26.02671571989103	32.547513587796935	22.571475442460624
18	20.223697763291298	27.607229971950375	33.80235100486498	18.366721259893342
19	24.232131475550034	27.75541158721066	30.534018572454357	17.47843836478495
20	24.925438561105416	26.925218036741512	30.06338730801606	18.08595609413701
21	22.53328707700334	28.22900110531114	30.691074948701193	18.546636868984322
22	20.3794094787826	27.432424073730438	29.715926968785045	22.47223947870192
23	19.29937795993449	29.714044443727296	30.423487458349136	20.563090137989086
24	18.736234035515178	28.152355442245476	34.0578365484172	19.053573973822143
25	19.350744000796038	29.836946436783464	30.83091966727714	19.98138989514335
26	18.44982129458559	31.495451012664017	30.66095454777714	19.393773144973256
27	19.959606390903637	29.761914366624442	30.738675939447234	19.53980330302468
28	17.34155189987118	30.12255238125973	33.582095573107864	18.953800145761228
29	19.245322597561863	28.42343905056193	31.80230259707778	20.52893575479842
30	19.849075276798416	29.684461907105458	31.27116159864028	19.19530121745585
31	21.831374162612516	29.875134802240744	28.758528510842	19.534962524304746
32	22.45099383876442	29.950166872399762	29.01293832578979	18.585900963046033
33	21.061152481840356	30.572744802213847	28.4575934337526	19.908509282193197
34	20.114242377790507	28.745081903286618	30.043755260985204	21.096920457937667
35	20.507152250558708	28.91719847999548	30.341732084412428	20.233917185033388
36	21.818465419359352	31.382499509198823	28.03456316006035	18.764471911381477
37	19.821644197385442	32.595114578542976	29.07075873827792	18.51248248579366
38	19.89882772475332	30.53159818309439	29.48088026871701	20.088693823435285
39	21.38763611328498	29.599479347355455	28.987658703585673	20.025225835773895
40	21.576426483362514	28.335498237149746	32.028205604008164	18.059869675479572
41	19.150927412523096	28.811777076761302	31.041224609443287	20.99607090127232
42	20.194922023122786	29.891270731307202	29.344531668105457	20.56927557746456
43	20.25731428217975	28.813928533970163	30.079254304931407	20.849502878918678
44	19.480369297629903	30.425101051255776	29.7073211399496	20.38720851116472
45	18.927175862801572	30.563063244773975	29.67962112838552	20.83013976403893
46	20.50096681108323	30.628951621795338	28.777891625721747	20.092189941399685
47	19.792061660763604	29.73125610139818	30.338504898599133	20.138177339239082
48	19.383284791080058	29.23050443603583	30.64965939743062	20.736551375453487
49	19.702507254444775	28.818231448387888	31.33597424705721	20.14328705011013
50	18.864245739442396	29.356095750603078	31.01460032648363	20.76505818347089
51	18.975045785698725	28.543920654258137	30.0009950489591	22.48003851108404
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	380.0
1	553.0
2	726.0
3	2539.5
4	4353.0
5	3904.5
6	3456.0
7	2729.5
8	2003.0
9	1950.0
10	1897.0
11	1910.0
12	1923.0
13	1906.5
14	1890.0
15	1944.0
16	1998.0
17	1884.5
18	1771.0
19	1838.0
20	1905.0
21	2228.5
22	2552.0
23	2895.0
24	3238.0
25	3865.5
26	5465.0
27	6437.0
28	7337.0
29	8237.0
30	9629.0
31	11021.0
32	11742.5
33	12464.0
34	13726.0
35	14988.0
36	16090.5
37	17193.0
38	18935.5
39	20678.0
40	22984.5
41	25291.0
42	26919.0
43	28547.0
44	29929.5
45	31312.0
46	32779.5
47	34247.0
48	33301.0
49	32355.0
50	29691.5
51	27028.0
52	24247.0
53	21466.0
54	18362.0
55	15258.0
56	12996.5
57	10735.0
58	9391.0
59	8047.0
60	6795.5
61	5544.0
62	4438.5
63	3333.0
64	2505.0
65	1677.0
66	1351.5
67	1026.0
68	893.5
69	761.0
70	674.0
71	587.0
72	517.5
73	448.0
74	364.5
75	210.5
76	140.0
77	104.0
78	68.0
79	52.0
80	36.0
81	32.0
82	28.0
83	20.0
84	12.0
85	9.5
86	7.0
87	4.5
88	2.0
89	2.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	371841.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.374981136957174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.89690305715756	35.66092428766107
2	9.283477476924597	8.6104218575549
3	3.820212867529561	5.314868990125333
4	2.0931809359316706	3.882849056802784
5	1.235536732733126	2.8648996337258215
6	0.9304158342806065	2.5888810058573646
7	0.669104245155596	2.1720787723424113
8	0.5738436866560853	2.1289592115390334
9	0.44718757500835776	1.8664483820124793
>10	3.988787402424913	30.994579594307677
>50	0.04701603054424502	1.5156213315678784
>100	0.012040690749135918	1.2925325075002556
>500	0.0017200986784479885	0.530201567569535
>1k	5.733662261493295E-4	0.5767338014334611
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2169	0.583313835752378	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	936	0.2517204934367108	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	544	0.14629909020253282	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	514	0.1382311256693049	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	462	0.12424665381170984	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	433	0.11644762142958953	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	392	0.10542140323417805	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.689321511075971E-4	0.0	0.0	0.07772139167009555	0.0
2	2.689321511075971E-4	0.0	0.0	0.22751659983702713	0.0
3	2.689321511075971E-4	0.0	0.0	0.3071205165648758	0.0
4	2.689321511075971E-4	0.0	0.0	0.44723416729193394	0.0
5	2.689321511075971E-4	0.0	0.0	0.8619275442998486	0.0
6	2.689321511075971E-4	0.0	0.0	1.1816878719667816	0.0
7	2.689321511075971E-4	0.0	0.0	1.4600326483631445	0.0
8	2.689321511075971E-4	0.0	0.0	2.3362135966716955	0.0
9	2.689321511075971E-4	0.0	0.0	2.7363846375198	0.0
10	2.689321511075971E-4	0.0	0.0	3.531348076193857	0.0
11	2.689321511075971E-4	0.0	0.0	4.165490088505571	0.0
12	2.689321511075971E-4	0.0	0.0	4.862293292025355	0.0
13	2.689321511075971E-4	0.0	0.0	5.1180477677286795	0.0
14	2.689321511075971E-4	0.0	0.0	5.247404132411434	0.0
15	2.689321511075971E-4	0.0	0.0	5.438614891848935	0.0
16	2.689321511075971E-4	0.0	0.0	5.848736422288021	0.0
17	2.689321511075971E-4	0.0	0.0	6.281179321269037	0.0
18	8.067964533227912E-4	0.0	0.0	6.87847762887901	0.0
19	8.067964533227912E-4	0.0	0.0	7.157898133879804	0.0
20	8.067964533227912E-4	0.0	0.0	7.527948773803857	0.0
21	8.067964533227912E-4	0.0	0.0	7.921934375176487	0.0
22	8.067964533227912E-4	0.0	0.0	8.375622914095002	0.0
23	8.067964533227912E-4	0.0	0.0	8.825546402898013	0.0
24	8.067964533227912E-4	0.0	0.0	9.192638789159883	0.0
25	8.067964533227912E-4	0.0	0.0	9.493036001947068	0.0
26	8.067964533227912E-4	0.0	0.0	9.779986607178875	0.0
27	8.067964533227912E-4	0.0	0.0	10.100553731299131	0.0
28	8.067964533227912E-4	0.0	0.0	10.423272312628248	0.0
29	8.067964533227912E-4	0.0	0.0	10.790633631041224	0.0
30	8.067964533227912E-4	0.0	0.0	11.208823126013538	0.0
31	8.067964533227912E-4	0.0	0.0	11.622440774417022	0.0
32	8.067964533227912E-4	0.0	0.0	12.030679779798355	0.0
33	8.067964533227912E-4	0.0	0.0	12.445642088957378	0.0
34	8.067964533227912E-4	0.0	0.0	12.862217991023044	0.0
35	8.067964533227912E-4	0.0	0.0	13.29358516139963	0.0
36	8.067964533227912E-4	0.0	0.0	13.781965947811027	0.0
37	8.067964533227912E-4	0.0	0.0	14.23269623306736	0.0
38	8.067964533227912E-4	0.0	0.0	14.675896418092679	0.0
39	8.067964533227912E-4	0.0	0.0	15.145451953926544	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACCA	25	3.8856975E-5	45.000004	23
CGACGGT	25	3.8856975E-5	45.000004	28
CGGTTTA	65	0.0	45.000004	1
TACGGGT	30	2.1615251E-6	45.000004	4
CGGTCTA	25	3.8856975E-5	45.000004	31
ATAACGC	30	2.1615251E-6	45.000004	11
GGCGAGC	25	3.8856975E-5	45.000004	9
GTAACGG	35	1.2089549E-7	45.000004	2
AGTAACG	25	3.8856975E-5	45.000004	1
TCGCCGT	20	7.0271234E-4	45.0	25
ATGGGTA	80	0.0	45.0	5
ACACGCG	45	3.8380676E-10	45.0	36
GCACGTA	20	7.0271234E-4	45.0	37
TCGGGCG	20	7.0271234E-4	45.0	5
ACGGGTC	20	7.0271234E-4	45.0	5
GCTCTCG	20	7.0271234E-4	45.0	17
GAGCGCT	20	7.0271234E-4	45.0	36
CATACGG	20	7.0271234E-4	45.0	2
TTACGCT	20	7.0271234E-4	45.0	14
CACGTAA	20	7.0271234E-4	45.0	38
>>END_MODULE