SRR3549478_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549478_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	143417
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1291	0.9001722250500289	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCG	1080	0.7530488017459576	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGC	935	0.6519450274374725	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTC	930	0.6484586903923524	RNA PCR Primer, Index 41 (95% over 21bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	551	0.38419434237224315	No Hit
GCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	347	0.2419517909313401	Illumina PCR Primer Index 3 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	280	0.19523487452672975	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCT	260	0.18128952634624904	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	260	0.18128952634624904	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTC	260	0.18128952634624904	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCT	242	0.1687387129838164	TruSeq Adapter, Index 11 (95% over 21bp)
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	171	0.11923272694310996	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGATC	60	0.0	45.000004	16
CTTCGGG	20	7.012626E-4	45.000004	3
GTTTCGA	20	7.012626E-4	45.000004	33
GGGTCTA	20	7.012626E-4	45.000004	7
TTTCGAT	20	7.012626E-4	45.000004	34
TGGGTAC	20	7.012626E-4	45.000004	6
ATATGTC	20	7.012626E-4	45.000004	31
GTAGGGC	45	3.8016879E-10	45.000004	4
TACGGGA	40	6.7429937E-9	45.000004	4
TCGATGA	20	7.012626E-4	45.000004	36
TCGATAG	20	7.012626E-4	45.000004	1
ACGGGAT	25	3.8736915E-5	45.000004	5
ACGGGAA	25	3.8736915E-5	45.000004	5
AGACGAG	25	3.8736915E-5	45.000004	1
ACCTATG	20	7.012626E-4	45.000004	34
TAACCTC	20	7.012626E-4	45.000004	28
GCTTCGG	20	7.012626E-4	45.000004	2
CGGATGT	30	2.1521864E-6	45.000004	6
TACGAGG	25	3.8736915E-5	45.000004	2
CTACGAA	25	3.8736915E-5	45.000004	11