##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549477_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	295854
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69391997404125	31.0	31.0	34.0	30.0	34.0
2	31.866008233790993	31.0	31.0	34.0	30.0	34.0
3	31.594881258999372	31.0	31.0	34.0	30.0	34.0
4	35.53830605636564	37.0	35.0	37.0	33.0	37.0
5	35.356895630953105	37.0	35.0	37.0	33.0	37.0
6	35.39629006199004	37.0	35.0	37.0	33.0	37.0
7	32.122742974575296	37.0	35.0	37.0	10.0	37.0
8	33.71476471502836	37.0	35.0	37.0	19.0	37.0
9	36.685496900498215	39.0	35.0	39.0	32.0	39.0
10	36.51272925159031	38.0	35.0	39.0	32.0	39.0
11	36.61998823744144	38.0	35.0	39.0	32.0	39.0
12	36.66141407586174	39.0	35.0	39.0	33.0	39.0
13	32.92921846586492	38.0	35.0	39.0	7.0	39.0
14	35.518262386176964	39.0	35.0	40.0	18.0	41.0
15	36.90611923448728	39.0	35.0	41.0	31.0	41.0
16	37.479172159240704	39.0	36.0	41.0	32.0	41.0
17	37.627620380322725	39.0	36.0	41.0	33.0	41.0
18	37.58229058927714	39.0	36.0	40.0	33.0	41.0
19	37.652220351930346	39.0	36.0	41.0	33.0	41.0
20	37.63100380593131	39.0	36.0	41.0	33.0	41.0
21	37.53808973344961	39.0	36.0	41.0	33.0	41.0
22	37.54485658466676	39.0	36.0	41.0	33.0	41.0
23	37.42610206385582	39.0	36.0	40.0	32.0	41.0
24	37.38453088347631	39.0	35.0	40.0	32.0	41.0
25	37.20930594144409	39.0	35.0	40.0	32.0	41.0
26	37.11539475552131	39.0	35.0	40.0	32.0	41.0
27	37.101110682972006	39.0	35.0	40.0	32.0	41.0
28	36.986030271688065	39.0	35.0	40.0	31.0	41.0
29	36.96591562054257	39.0	35.0	40.0	31.0	41.0
30	36.82329797805674	39.0	35.0	40.0	31.0	41.0
31	36.72298160579204	39.0	35.0	40.0	31.0	41.0
32	36.6000966693031	39.0	35.0	40.0	30.0	41.0
33	36.442157280280135	39.0	35.0	40.0	30.0	41.0
34	36.33874816632528	39.0	35.0	40.0	30.0	41.0
35	36.20378632703969	39.0	35.0	40.0	30.0	41.0
36	36.075239814232695	39.0	35.0	40.0	29.0	41.0
37	36.03368215403544	39.0	35.0	40.0	29.0	41.0
38	35.9272005786638	38.0	35.0	40.0	29.0	41.0
39	35.8332995328777	38.0	35.0	40.0	28.0	41.0
40	35.77373975001183	38.0	35.0	40.0	28.0	41.0
41	35.76061841313621	38.0	35.0	40.0	28.0	41.0
42	35.71380816213403	38.0	35.0	40.0	28.0	41.0
43	35.66803896516525	38.0	35.0	40.0	27.0	41.0
44	35.46331974555017	38.0	35.0	40.0	26.0	41.0
45	35.41227429745753	38.0	34.0	40.0	26.0	41.0
46	35.334678591467416	38.0	34.0	40.0	26.0	41.0
47	35.21670824122709	38.0	34.0	40.0	26.0	41.0
48	35.15684763430611	38.0	34.0	40.0	26.0	41.0
49	35.091822317764844	38.0	34.0	40.0	25.0	41.0
50	34.896918750464756	38.0	34.0	40.0	24.0	41.0
51	32.999665375489265	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	10.0
10	8.0
11	10.0
12	4.0
13	3.0
14	5.0
15	7.0
16	13.0
17	49.0
18	104.0
19	168.0
20	377.0
21	598.0
22	947.0
23	1385.0
24	2052.0
25	2774.0
26	3429.0
27	4068.0
28	4414.0
29	5155.0
30	6369.0
31	8126.0
32	10935.0
33	15261.0
34	21555.0
35	27301.0
36	32116.0
37	46402.0
38	54654.0
39	47552.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.013398500611785	23.487260608273	28.18214389529971	14.317196995815504
2	31.914390205979977	25.385494196461767	29.489545519073594	13.210570078484658
3	30.367343351788385	25.909063254172665	30.275406112474396	13.448187281564556
4	26.119302088192146	27.981369188856664	30.577920190364168	15.32140853258702
5	22.192703157638565	33.3265732422073	28.758441663793626	15.722281936360503
6	21.73132693828713	42.07244113650652	26.139920366126535	10.056311559079816
7	77.06571484583613	13.725351017731718	6.975400028392382	2.2335341080397764
8	84.12088394951563	4.556639423499429	7.705151865447146	3.6173247615377857
9	76.43060428454575	7.87043609347854	11.037876790579137	4.661082831396567
10	29.031549345285175	41.34674535412737	17.109790639977827	12.511914660609625
11	26.76962285451608	27.042392531451327	30.69554577595706	15.492438838075536
12	26.436012357446582	23.621448417124665	31.93467047935806	18.007868746070695
13	20.28838548743637	34.6336368614249	29.542612234412918	15.535365416725819
14	17.817572180872997	31.91675623787409	31.26440744421235	19.001264137040565
15	16.982362922252193	27.92289440061652	38.097845558958134	16.996897118173155
16	18.92352308909124	27.398987338349322	34.98076754074645	18.69672203181299
17	18.39894001771144	25.85126447504512	32.89358940558519	22.85620610165825
18	19.430529923543368	26.47691090875905	34.95372717624234	19.138831991455245
19	22.2957945473105	28.03173186774558	30.964935407329293	18.707538177614637
20	23.37774713203134	26.315344730846974	31.289757785934952	19.017150351186736
21	21.869232797258107	28.63912605541923	31.447605913727717	18.04403523359495
22	20.619629952611763	25.952665841935552	29.63116942816389	23.796534777288798
23	18.221487625653193	29.051153609550656	30.794581110953374	21.932777653842773
24	17.63437371135763	26.67768561520209	35.24542510833046	20.44251556510982
25	17.916945520425614	29.439182840184685	31.885321814138056	20.75854982525164
26	17.976096317778364	30.02832478181806	31.400623280401817	20.594955620001755
27	18.590926605690644	30.013790585897098	30.625240828246366	20.770041980165892
28	16.8647373366593	28.76148370480034	34.573134045846935	19.800644912693425
29	18.021050923766452	26.37145348719301	33.42628458631622	22.181211002724314
30	20.013587783163317	28.36669438304028	32.239212584585644	19.38050524921076
31	21.044501679882647	28.45660359501646	30.544795743846624	19.95409898125427
32	20.901187748010845	27.68764322943073	31.44997194562183	19.961197076936596
33	21.123256741500875	27.814056933487464	29.89785502308571	21.16483130192595
34	18.556112136391597	26.97479162019104	33.140670736241525	21.328425507175837
35	18.936029257674395	27.414535547939185	32.23448052079742	21.414954673589
36	20.489498198435715	27.713331575709642	31.017664118112314	20.779506107742332
37	18.967801685966727	29.949569720199825	31.76465418753845	19.317974406294997
38	18.18971519736086	29.614945209461425	29.891432936515983	22.30390665666173
39	18.96644966774152	28.283883266746436	30.233831552049324	22.51583551346272
40	19.506242944154888	26.659095364605516	32.221298343101665	21.61336334813793
41	17.56575878642844	26.72973831687251	31.50574269741156	24.198760199287488
42	19.806390990150547	26.127752202099686	31.080194961028074	22.985661846721694
43	19.503538907704478	26.106119910496396	31.851521358507913	22.538819823291217
44	18.75891487017245	28.056068195799277	31.30970005475674	21.875316879271534
45	18.15828077362483	28.945696187984616	31.098447207068354	21.797575831322206
46	19.333522615884863	29.1086143841219	30.414325985114278	21.14353701487896
47	18.208981457070042	27.14717394390476	32.29194129536866	22.351903303656535
48	18.537183881238718	26.396803828915615	32.31526360975346	22.750748680092208
49	19.301074178479926	26.175072839981883	32.083392484130684	22.440460497407503
50	18.146112609597978	27.257025424702725	32.439987291028686	22.156874674670615
51	17.75842138352025	26.254165906156413	30.828719571139818	25.15869313918352
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	199.0
1	322.5
2	446.0
3	1541.5
4	2637.0
5	2329.5
6	2022.0
7	1636.0
8	1250.0
9	1216.0
10	1182.0
11	1202.5
12	1223.0
13	1194.0
14	1165.0
15	1184.5
16	1204.0
17	1179.0
18	1154.0
19	1169.0
20	1184.0
21	1454.5
22	1725.0
23	1950.5
24	2176.0
25	2668.5
26	3706.0
27	4251.0
28	5332.5
29	6414.0
30	7189.5
31	7965.0
32	9276.0
33	10587.0
34	11907.5
35	13228.0
36	13811.5
37	14395.0
38	15667.5
39	16940.0
40	18927.0
41	20914.0
42	22410.0
43	23906.0
44	25056.5
45	26207.0
46	27791.0
47	29375.0
48	29067.5
49	28760.0
50	26354.0
51	23948.0
52	20048.0
53	16148.0
54	13601.5
55	11055.0
56	9302.5
57	7550.0
58	6159.0
59	4768.0
60	3989.0
61	3210.0
62	2668.5
63	2127.0
64	1670.0
65	1213.0
66	971.0
67	729.0
68	597.0
69	465.0
70	412.5
71	360.0
72	317.0
73	274.0
74	214.5
75	117.5
76	80.0
77	58.5
78	37.0
79	32.0
80	27.0
81	22.0
82	17.0
83	13.5
84	10.0
85	9.0
86	8.0
87	4.5
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	295854.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.18794755801031
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.26327628216883	38.776852582095515
2	9.072055863752054	9.10615727866656
3	3.7698195488690094	5.675985174653996
4	2.117538240569403	4.250995926791146
5	1.3547798703663156	3.3996810543296325
6	0.923289825453883	2.7802812804434387
7	0.7528251918359935	2.6447925873749725
8	0.6654560095888267	2.671829704912547
9	0.5706942820129891	2.577777722759182
>10	3.484997576016471	26.2445105231721
>50	0.0159583006523491	0.5613074780087535
>100	0.00864407952002243	0.89034979270354
>500	0.0	0.0
>1k	6.649291938478792E-4	0.4194788940885869
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1257	0.4248717272708836	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	485	0.1639322098061882	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12742771772563494	0.0
2	0.0	0.0	0.0	0.4532641100002028	0.0
3	0.0	0.0	0.0	0.614830287912281	0.0
4	0.0	0.0	0.0	0.8598835912308098	0.0
5	0.0	0.0	0.0	1.4723478472489808	0.0
6	0.0	0.0	0.0	2.0665598572268755	0.0
7	0.0	0.0	0.0	2.4532370696356987	0.0
8	0.0	0.0	0.0	3.7795669485624668	0.0
9	0.0	0.0	0.0	4.364652835520223	0.0
10	0.0	0.0	0.0	5.452013493141887	0.0
11	0.0	0.0	0.0	6.276068601404747	0.0
12	0.0	0.0	0.0	7.071393322381986	0.0
13	0.0	0.0	0.0	7.37965347772888	0.0
14	0.0	0.0	0.0	7.51553130936205	0.0
15	0.0	0.0	0.0	7.745374407647015	0.0
16	0.0	0.0	0.0	8.27299952003353	0.0
17	0.0	0.0	0.0	8.85639538420978	0.0
18	0.0	0.0	0.0	9.657466182644143	0.0
19	0.0	0.0	0.0	9.993104707051451	0.0
20	0.0	0.0	0.0	10.417638429766033	0.0
21	0.0	0.0	0.0	10.886788753912402	0.0
22	0.0	0.0	0.0	11.392781574695627	0.0
23	0.0	0.0	0.0	11.905196482048577	0.0
24	0.0	0.0	0.0	12.268213375516302	0.0
25	0.0	0.0	0.0	12.620076118626079	0.0
26	0.0	0.0	0.0	12.94929255646366	0.0
27	0.0	0.0	0.0	13.305211354249055	0.0
28	0.0	0.0	0.0	13.634427792086637	0.0
29	0.0	0.0	0.0	14.001500740229979	0.0
30	0.0	0.0	0.0	14.440568658865521	0.0
31	0.0	0.0	0.0	14.82116178926092	0.0
32	0.0	0.0	0.0	15.196684851311796	0.0
33	0.0	0.0	0.0	15.581672040939113	0.0
34	0.0	0.0	0.0	15.959561134884098	0.0
35	0.0	0.0	0.0	16.366518620671005	0.0
36	0.0	0.0	0.0	16.790038329716683	0.0
37	0.0	0.0	0.0	17.185841665145645	0.0
38	0.0	0.0	0.0	17.57725094134269	0.0
39	0.0	0.0	0.0	17.989278495474117	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAC	30	2.160019E-6	45.000004	6
CGACGGT	30	2.160019E-6	45.000004	28
CGGTTTA	60	0.0	45.000004	1
ATTCGGG	30	2.160019E-6	45.000004	3
TCGTTCC	20	7.024786E-4	45.0	44
CTCACGT	25	3.8837607E-5	45.0	45
AATCACG	20	7.024786E-4	45.0	1
ATAGCGG	25	3.8837607E-5	45.0	2
TAACGCA	20	7.024786E-4	45.0	13
CGTTCCC	20	7.024786E-4	45.0	45
AACGGGC	45	3.8198777E-10	45.0	4
CTACGGG	35	1.2078453E-7	45.0	3
TCGTAGA	20	7.024786E-4	45.0	42
ATAACGC	20	7.024786E-4	45.0	12
CGTTTGG	35	1.2078453E-7	45.0	2
GTAACGG	35	1.2078453E-7	45.0	2
CTAGCGG	35	1.2078453E-7	45.0	2
CGGTTTT	190	0.0	42.63158	1
TAACGGG	80	0.0	42.1875	3
TACGGGA	155	0.0	42.096775	4
>>END_MODULE