##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549474_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	296697
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.616979612196957	31.0	31.0	34.0	30.0	34.0
2	31.73044553871458	31.0	31.0	34.0	30.0	34.0
3	31.331573962662244	31.0	31.0	34.0	28.0	34.0
4	35.39304745245149	37.0	35.0	37.0	33.0	37.0
5	35.28527420230066	37.0	35.0	37.0	33.0	37.0
6	35.33263228141842	37.0	35.0	37.0	33.0	37.0
7	32.12543436569969	37.0	35.0	37.0	11.0	37.0
8	33.71486061537528	37.0	35.0	37.0	19.0	37.0
9	36.64330613386721	39.0	35.0	39.0	32.0	39.0
10	36.4499068072815	38.0	35.0	39.0	32.0	39.0
11	36.54186931448582	38.0	35.0	39.0	32.0	39.0
12	36.521646663093996	38.0	35.0	39.0	32.0	39.0
13	32.7932200190767	37.0	34.0	39.0	10.0	39.0
14	35.340802232580714	39.0	34.0	40.0	18.0	41.0
15	36.69796459013741	39.0	35.0	40.0	30.0	41.0
16	37.30501151005908	39.0	36.0	41.0	32.0	41.0
17	37.422441076249505	39.0	36.0	40.0	32.0	41.0
18	37.37872981526608	39.0	36.0	40.0	32.0	41.0
19	37.40151737294276	39.0	36.0	40.0	32.0	41.0
20	37.33221434662298	39.0	35.0	40.0	32.0	41.0
21	37.244785083772335	39.0	35.0	40.0	32.0	41.0
22	37.23487935503224	39.0	35.0	40.0	32.0	41.0
23	37.129060961182624	39.0	35.0	40.0	32.0	41.0
24	37.05616504379889	39.0	35.0	40.0	32.0	41.0
25	36.84481474366104	39.0	35.0	40.0	31.0	41.0
26	36.73288910909109	39.0	35.0	40.0	31.0	41.0
27	36.707947839041175	39.0	35.0	40.0	31.0	41.0
28	36.51429572931307	38.0	35.0	40.0	31.0	41.0
29	36.44178067186389	38.0	35.0	40.0	31.0	41.0
30	36.25341678547474	38.0	35.0	40.0	30.0	41.0
31	36.131969652541144	38.0	35.0	40.0	30.0	41.0
32	36.006686956726895	38.0	35.0	40.0	30.0	41.0
33	35.72301371432808	38.0	35.0	40.0	29.0	41.0
34	35.5780375265001	38.0	35.0	40.0	27.0	41.0
35	35.38803897579012	38.0	34.0	40.0	26.0	41.0
36	35.19018055457251	38.0	34.0	40.0	25.0	41.0
37	35.10672504272035	38.0	34.0	40.0	24.0	41.0
38	34.97517332497464	38.0	34.0	40.0	24.0	41.0
39	34.852492610306136	38.0	34.0	40.0	24.0	41.0
40	34.76990330202193	38.0	34.0	40.0	23.0	41.0
41	34.68257178198633	38.0	34.0	40.0	23.0	41.0
42	34.66018193645369	38.0	34.0	40.0	23.0	41.0
43	34.57925425602551	38.0	34.0	40.0	23.0	41.0
44	34.39917828626511	38.0	33.0	40.0	22.0	41.0
45	34.34961256770375	38.0	33.0	40.0	22.0	41.0
46	34.26560767382212	38.0	33.0	40.0	22.0	41.0
47	34.07380593669636	37.0	33.0	40.0	21.0	41.0
48	33.96844592294496	37.0	33.0	40.0	20.0	41.0
49	33.86362517989733	37.0	33.0	40.0	20.0	41.0
50	33.67502199213339	37.0	33.0	40.0	20.0	41.0
51	31.788332878323676	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	5.0
10	9.0
11	7.0
12	6.0
13	6.0
14	5.0
15	13.0
16	29.0
17	61.0
18	190.0
19	315.0
20	651.0
21	965.0
22	1507.0
23	2251.0
24	3098.0
25	4108.0
26	5032.0
27	5438.0
28	5832.0
29	6374.0
30	7504.0
31	9133.0
32	12005.0
33	16326.0
34	22986.0
35	27580.0
36	30172.0
37	42513.0
38	49635.0
39	42938.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.42367128754251	22.68004058012046	27.084534053259723	15.811754079077309
2	32.532179294027245	25.806799529486312	28.599884730887066	13.061136445599383
3	30.764045474002096	25.14417065221421	31.201528832445224	12.890255041338468
4	26.250349683346986	27.4471936015531	32.00066060661213	14.301796108487782
5	23.21931128390243	32.76372865246363	29.299588469044174	14.717371594589768
6	21.057172805926587	41.93031948418757	27.397985149832994	9.61452256005285
7	75.21579254256025	13.939810648574136	8.76854164349488	2.0758551653707316
8	82.5404368766789	4.9633127399333326	9.59396286447116	2.902287518916605
9	75.33982480443011	8.349258671304396	12.235378180433235	4.07553834383226
10	31.727991857012373	36.96633265587451	18.965139519442396	12.340535967670721
11	28.679764203884773	27.381469984529673	29.345763523055506	14.59300228853005
12	28.453607552486204	23.16673239028369	31.85067594212277	16.52898411510733
13	20.36690630508566	35.301334357947674	29.417890979686344	14.913868357280325
14	17.804022285361835	32.0734621516227	32.93292483577522	17.189590727240248
15	17.488885967839245	27.638297657205836	39.465852367903956	15.406964007050963
16	19.711018311610832	26.35281111706556	36.49851532034365	17.437655250979955
17	19.091531090641293	24.98677101554785	33.203571320235795	22.718126573575063
18	21.03526493358544	26.031608004125424	34.6050010616892	18.32812600059994
19	24.296841558896787	26.569193486958078	31.506216780082035	17.6277481740631
20	24.97598560147221	25.86072659986451	30.91841171295969	18.244876085703595
21	22.35512998109182	27.70166196490022	32.393654131993245	17.549553922014717
22	19.942904714237084	26.259449876473305	31.58946669497838	22.208178714311234
23	18.880878471976462	28.297893136769165	31.591151915927696	21.23007647532668
24	18.497659228101394	26.66356586011992	35.73814362801107	19.100631283767612
25	18.52900433775872	28.294859739060392	32.79709602726014	20.37903989592075
26	17.091173823800037	29.97738433486014	31.851687074692364	21.079754766647454
27	18.16567070108562	29.756957434689262	31.6292379093823	20.44813395484282
28	17.147797247697145	29.674381608172645	34.56792620080419	18.60989494332602
29	19.57350428214643	27.092960158006317	33.3754638570663	19.958071702780952
30	20.188609928647743	27.81659403364375	33.426357529735725	18.568438507972783
31	22.689477817436643	28.2780075295672	30.802805555836425	18.22970909715973
32	23.43940113988345	27.738399781595362	31.011098865172215	17.811100213348972
33	21.457244259294836	28.378446698146593	30.6218128258796	19.54249621667897
34	20.533406134878344	27.488650036906336	31.773492822643977	20.204451005571343
35	19.540136907349922	27.531791693208895	32.37275739222169	20.555314007219486
36	21.629473840315203	30.488680370883426	30.46744658692201	17.41439920187936
37	20.46363798757655	30.964249722781155	30.528788629477212	18.043323660165083
38	20.00660606612133	30.883696161403723	29.349133964954145	19.760563807520803
39	21.145815427860747	29.036694000950465	29.64101423337614	20.17647633781265
40	21.3692757257404	27.709751025456946	31.657886665520717	19.263086583281936
41	18.948287309949208	28.556743074584507	30.806175997735064	21.68879361773122
42	20.522620720802703	28.195094658860725	30.530136806236662	20.752147814099906
43	19.948634465464767	27.181265735750614	31.65653848876126	21.21356131002336
44	19.094227444160204	28.873901657246282	30.929197127035323	21.102673771558187
45	18.89503432795074	29.944016960063635	30.347795899520385	20.81315281246524
46	20.103674792802085	29.841555526345058	30.25241239378895	19.802357287063906
47	19.548563012096515	28.650441359366624	30.863473510011897	20.93752211852496
48	19.11815758164053	28.350809074577764	32.32927869172927	20.201754652052433
49	20.392521663515303	27.5233655884623	31.794389562415525	20.289723185606864
50	19.10197946052707	28.66426017115104	32.07885485866052	20.154905509661372
51	18.94390573548098	28.079488501737465	30.102764773489454	22.87384098929211
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	376.0
1	529.5
2	683.0
3	2519.5
4	4356.0
5	3825.5
6	3295.0
7	2554.5
8	1814.0
9	1801.0
10	1788.0
11	1769.5
12	1751.0
13	1746.0
14	1741.0
15	1713.5
16	1686.0
17	1657.0
18	1628.0
19	1641.0
20	1654.0
21	1810.5
22	1967.0
23	2076.5
24	2186.0
25	2861.0
26	4255.0
27	4974.0
28	5647.5
29	6321.0
30	7164.0
31	8007.0
32	8948.5
33	9890.0
34	11007.0
35	12124.0
36	12706.0
37	13288.0
38	14373.0
39	15458.0
40	17410.0
41	19362.0
42	20732.5
43	22103.0
44	23421.5
45	24740.0
46	26021.0
47	27302.0
48	26270.5
49	25239.0
50	23862.5
51	22486.0
52	19672.0
53	16858.0
54	14546.5
55	12235.0
56	10551.0
57	8867.0
58	7635.0
59	6403.0
60	5499.0
61	4595.0
62	3802.0
63	3009.0
64	2321.5
65	1634.0
66	1346.5
67	1059.0
68	891.0
69	723.0
70	641.0
71	559.0
72	477.5
73	396.0
74	336.0
75	211.0
76	146.0
77	117.5
78	89.0
79	64.0
80	39.0
81	32.5
82	26.0
83	20.5
84	15.0
85	11.0
86	7.0
87	5.0
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	296697.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.58562079499336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.66169087347691	37.732414630767465
2	8.728254909891083	8.481353665080144
3	3.651931892872242	5.322941343486992
4	2.0048717014033723	3.896317449079896
5	1.307584172764615	3.176489438773833
6	0.9458370970260538	2.7572449517967113
7	0.7434882848459224	2.5285987911130796
8	0.6139532143015842	2.386343844473925
9	0.5397079044993657	2.3599839229260002
>10	3.7711399441551094	28.63365465208842
>50	0.017826959214271673	0.5773115440697759
>100	0.01234174099449577	1.1039875689828258
>500	6.856522774719874E-4	0.30148121603182176
>1k	6.856522774719874E-4	0.7418769813291349
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2227	0.7505974108265334	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	905	0.3050249918266784	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07785720785852233	0.0
2	0.0	0.0	0.0	0.27064648446057765	0.0
3	0.0	0.0	0.0	0.36265954829337677	0.0
4	0.0	0.0	0.0	0.536574350263063	0.0
5	0.0	0.0	0.0	0.9558573224535469	0.0
6	0.0	0.0	0.0	1.305372147342238	0.0
7	0.0	0.0	0.0	1.5679295712460861	0.0
8	0.0	0.0	0.0	2.3916655712730495	0.0
9	0.0	0.0	0.0	2.7250022750482814	0.0
10	0.0	0.0	0.0	3.429424631863484	0.0
11	0.0	0.0	0.0	3.984536412569052	0.0
12	0.0	0.0	0.0	4.58481211471636	0.0
13	0.0	0.0	0.0	4.869951499341078	0.0
14	0.0	0.0	0.0	5.01454345679262	0.0
15	0.0	0.0	0.0	5.1972214076987635	0.0
16	0.0	0.0	0.0	5.5656107072198235	0.0
17	0.0	0.0	0.0	5.943774288246932	0.0
18	0.0	0.0	0.0	6.5285459576605085	0.0
19	0.0	0.0	0.0	6.783351365197491	0.0
20	0.0	0.0	0.0	7.141292294832776	0.0
21	0.0	0.0	0.0	7.47665126374719	0.0
22	0.0	0.0	0.0	7.853803712204707	0.0
23	0.0	0.0	0.0	8.25589743071214	0.0
24	0.0	0.0	0.0	8.560585378348955	0.0
25	0.0	0.0	0.0	8.8157278300758	0.0
26	0.0	0.0	0.0	9.083003872637741	0.0
27	0.0	0.0	0.0	9.353987401288183	0.0
28	0.0	0.0	0.0	9.650586288368268	0.0
29	0.0	0.0	0.0	9.982574815384044	0.0
30	0.0	0.0	0.0	10.35871613127197	0.0
31	0.0	0.0	0.0	10.679245155832382	0.0
32	0.0	0.0	0.0	11.036511997087938	0.0
33	0.0	0.0	0.0	11.408608782697499	0.0
34	0.0	0.0	0.0	11.793850291711747	0.0
35	0.0	0.0	0.0	12.171002740169264	0.0
36	0.0	0.0	0.0	12.57343350286656	0.0
37	0.0	0.0	0.0	12.951260039703806	0.0
38	0.0	0.0	0.0	13.37054301189429	0.0
39	0.0	0.0	0.0	13.821508137931964	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTGCA	30	2.160039E-6	45.000004	19
CACGTGC	30	2.160039E-6	45.000004	18
TCACGAC	25	3.883786E-5	45.0	25
CTTAACG	20	7.024817E-4	45.0	18
CGGGTGA	20	7.024817E-4	45.0	6
CTCACGA	25	3.883786E-5	45.0	24
ATTAGCG	20	7.024817E-4	45.0	1
CGACGCG	20	7.024817E-4	45.0	15
TGCGGGT	35	1.2078635E-7	45.0	4
CTGCGGG	45	3.8198777E-10	45.0	3
GTTACGG	20	7.024817E-4	45.0	2
AATGCGG	45	3.8198777E-10	45.0	2
GCCGATA	20	7.024817E-4	45.0	9
CTACGGG	35	1.2078635E-7	45.0	3
GTATGCG	20	7.024817E-4	45.0	1
CTTTGCG	20	7.024817E-4	45.0	1
AACGACG	20	7.024817E-4	45.0	13
GCGATGA	35	1.2078635E-7	45.0	9
CAATGCG	25	3.883786E-5	45.0	1
GCTACGA	25	3.883786E-5	45.0	10
>>END_MODULE