FastQCFastQC Report
Sat 18 Jun 2016
SRR3549469_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549469_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences443218
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30720.6931126443420619No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12620.28473572824208404No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTC7200.16244827601767076No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCG6580.14845967447170466No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGC5350.1207080939853526No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACGT207.028591E-445.014
CTAAGCG253.8869133E-545.01
ACGGGTC253.8869133E-545.05
TATTGCG207.028591E-445.01
CGCAGGA207.028591E-445.03
TATACGG406.7993824E-945.02
TCGCGCG207.028591E-445.01
TCGGGTG302.1624728E-644.9999965
ACGGGAT1400.043.392865
GCGATGC950.042.631589
CGGTTTA1600.042.18751
CACAACG603.6379788E-1241.24999612
CGGTTTT5500.040.9090921
TAGGGCG2400.040.3124965
GCGGGTA900.040.05
CGGGCGA900.040.06
CGTTTTT16250.039.323081
GGCGATG3900.038.6538478
CGATCAT356.239603E-638.5714310
GTTGATC1900.037.89473716