##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549469_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	443218
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.633866404342783	31.0	31.0	34.0	30.0	34.0
2	31.77612371338709	31.0	31.0	34.0	30.0	34.0
3	31.379149763773132	31.0	31.0	34.0	28.0	34.0
4	35.42704718671173	37.0	35.0	37.0	33.0	37.0
5	35.31405312961116	37.0	35.0	37.0	33.0	37.0
6	35.355301454363314	37.0	35.0	37.0	33.0	37.0
7	32.08049988944492	37.0	35.0	37.0	10.0	37.0
8	33.67889616396446	37.0	35.0	37.0	19.0	37.0
9	36.660521458966016	39.0	35.0	39.0	32.0	39.0
10	36.49011096119742	38.0	35.0	39.0	32.0	39.0
11	36.55891231854302	38.0	35.0	39.0	32.0	39.0
12	36.59984025919525	39.0	35.0	39.0	32.0	39.0
13	32.920824515249834	38.0	35.0	39.0	10.0	39.0
14	35.48787729740218	39.0	35.0	40.0	18.0	41.0
15	36.85719668425019	39.0	35.0	41.0	31.0	41.0
16	37.43178751765497	39.0	36.0	41.0	32.0	41.0
17	37.56153405321986	39.0	36.0	41.0	33.0	41.0
18	37.515931212180014	39.0	36.0	40.0	33.0	41.0
19	37.51908541620602	39.0	36.0	40.0	32.0	41.0
20	37.45166938165869	39.0	35.0	40.0	32.0	41.0
21	37.38986458131213	39.0	35.0	40.0	32.0	41.0
22	37.395193787255934	39.0	35.0	40.0	32.0	41.0
23	37.29499478811781	39.0	35.0	40.0	32.0	41.0
24	37.18823468360942	39.0	35.0	40.0	32.0	41.0
25	37.00906326006615	39.0	35.0	40.0	32.0	41.0
26	36.90075312825743	39.0	35.0	40.0	31.0	41.0
27	36.89119575468505	39.0	35.0	40.0	31.0	41.0
28	36.757272944690875	39.0	35.0	40.0	31.0	41.0
29	36.6885776299699	39.0	35.0	40.0	31.0	41.0
30	36.56066089373626	38.0	35.0	40.0	31.0	41.0
31	36.47372850380626	38.0	35.0	40.0	30.0	41.0
32	36.294175778059554	38.0	35.0	40.0	30.0	41.0
33	36.09477954415209	38.0	35.0	40.0	30.0	41.0
34	35.91798618287164	38.0	35.0	40.0	29.0	41.0
35	35.80052479818058	38.0	35.0	40.0	28.0	41.0
36	35.62240928843143	38.0	35.0	40.0	27.0	41.0
37	35.55331010924647	38.0	35.0	40.0	27.0	41.0
38	35.414026506143706	38.0	35.0	40.0	26.0	41.0
39	35.27766697200926	38.0	34.0	40.0	25.0	41.0
40	35.19735209310091	38.0	34.0	40.0	25.0	41.0
41	35.12043734685866	38.0	34.0	40.0	24.0	41.0
42	35.086454070006184	38.0	34.0	40.0	24.0	41.0
43	35.04704682571556	38.0	34.0	40.0	24.0	41.0
44	34.85107103050869	38.0	34.0	40.0	23.0	41.0
45	34.83331001899742	38.0	34.0	40.0	23.0	41.0
46	34.769330216733074	38.0	34.0	40.0	23.0	41.0
47	34.59641305181649	38.0	33.0	40.0	23.0	41.0
48	34.49694958237256	38.0	33.0	40.0	23.0	41.0
49	34.41673172118461	38.0	33.0	40.0	23.0	41.0
50	34.24122215252991	37.0	33.0	40.0	22.0	41.0
51	32.38803478198088	35.0	30.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	8.0
10	12.0
11	17.0
12	8.0
13	5.0
14	7.0
15	16.0
16	41.0
17	88.0
18	216.0
19	387.0
20	711.0
21	1080.0
22	1719.0
23	2691.0
24	3872.0
25	5344.0
26	6726.0
27	7365.0
28	7921.0
29	8652.0
30	10458.0
31	13350.0
32	17477.0
33	23530.0
34	32520.0
35	40233.0
36	46762.0
37	66534.0
38	77370.0
39	68091.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.46344236921786	23.659463288945844	26.947235897459038	15.92985844437726
2	32.82064356592016	25.658479574385517	28.404532306900894	13.116344552793432
3	31.026041361136052	25.444589344295583	30.327965019471232	13.201404275097131
4	25.883650934754453	27.450148685297076	31.844374551575072	14.821825828373397
5	23.05434346078002	32.273283124782836	29.931546101467	14.740827312970142
6	21.37616252047525	41.70002120852492	27.197902612258527	9.725913658741296
7	75.4933238271009	13.651070128018267	8.804470937552175	2.0511351073286734
8	82.54425587408454	4.969563510507244	9.351380133478333	3.1348004819298856
9	75.5215266527984	7.914164135933108	12.037417252909403	4.526891958359092
10	30.921578094752473	36.52220803306725	18.794814290033347	13.761399582146936
11	27.892143369628492	27.627036808071875	29.416900938138795	15.063918884160843
12	25.790017553438716	24.5657892955611	32.27621621865538	17.367976932344806
13	20.241732059618517	34.487543375945926	30.225081111326705	15.045643453108854
14	18.16600408828161	31.346425461059795	32.25500769373085	18.23256275692774
15	17.44942669295922	27.298981539558408	38.535212920052885	16.716378847429482
16	19.867424156961132	26.619631874156735	35.39116191129422	18.121782057587914
17	19.46965150332342	26.065051509640853	32.548993948801716	21.916303038234005
18	21.151893650528635	26.452219900816303	34.015089639861195	18.380796808793868
19	23.76324968751269	27.527537239011053	30.838774598504575	17.870438474971685
20	24.487272628819227	26.940467219291637	30.90104643764468	17.67121371424446
21	22.026858114968253	28.654522153883644	31.746003095542147	17.572616635605957
22	20.792928085050697	25.638173539883308	31.26542694565654	22.303471429409456
23	18.77924633024832	28.730331349358558	31.848435758475514	20.641986561917612
24	18.521585314675846	27.737140639594966	34.531539784034045	19.209734261695147
25	19.370151934262598	29.527230392267462	31.693433028441987	19.409184645027956
26	18.38869359998917	29.979378093849977	31.371243947673605	20.260684358487246
27	19.78665126416346	28.720855199924188	31.322058219657144	20.170435316255205
28	17.923234164677424	29.374935133500895	33.60626147855006	19.095569223271617
29	19.23049154140852	27.52460414513851	33.08259141099865	20.162312902454325
30	19.942556484619306	28.581420429675692	32.197925174519085	19.278097911185917
31	21.49619374664386	29.004237192532795	29.63846233681845	19.86110672400489
32	21.90141194626572	28.85735687630015	30.40129236628476	18.839938811149366
33	21.48288201291464	28.7797426999806	29.882811618661698	19.854563668443067
34	18.91687611965218	29.27250247056753	30.607737050390554	21.20288435938974
35	19.505751120216235	27.80324806302993	31.252566457138474	21.438434359615357
36	20.65845701212496	30.586754148071606	29.116822872717265	19.637965967086174
37	19.246961991615862	30.731152615642866	30.198232021262672	19.823653371478596
38	19.03690734582079	30.65173345847867	28.94783154113777	21.363527654562766
39	19.46807214508436	29.474209079956136	29.39208245152498	21.665636323434516
40	20.077478802756204	28.4645479199852	30.926993037286394	20.530980239972205
41	17.735516156834784	29.106218610255	30.510493707385532	22.647771525524686
42	19.870357250833674	28.982126177185947	29.82482660902761	21.32268996295277
43	20.044989147552673	28.02571195213191	30.5391477782942	21.390151122021216
44	19.05901836116764	29.612515737176736	30.515006159497133	20.813459742158486
45	18.29099901177299	29.77000031587165	30.62691497186486	21.312085700490506
46	19.897206340897707	30.034430009611523	29.55430510493707	20.514058544553695
47	18.913943025779638	28.92414116755186	30.873294857158328	21.28862094951017
48	18.772252029475336	28.914439395511916	30.67677756769806	21.636531007314687
49	19.50597674282182	28.159731779846485	31.366505872956424	20.967785604375276
50	18.326196138243482	28.707317843589387	31.19864265440483	21.767843363762303
51	18.61724929944181	28.290141645871785	29.862279961553906	23.2303290931325
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	333.0
1	558.0
2	783.0
3	3379.5
4	5976.0
5	5335.0
6	4694.0
7	3683.5
8	2673.0
9	2556.5
10	2440.0
11	2400.0
12	2360.0
13	2403.5
14	2447.0
15	2365.5
16	2284.0
17	2323.5
18	2363.0
19	2481.5
20	2600.0
21	2721.5
22	2843.0
23	3160.5
24	3478.0
25	4596.5
26	6386.5
27	7058.0
28	8481.0
29	9904.0
30	11162.5
31	12421.0
32	14023.5
33	15626.0
34	17056.0
35	18486.0
36	19532.5
37	20579.0
38	22341.0
39	24103.0
40	26146.0
41	28189.0
42	30496.5
43	32804.0
44	34424.5
45	36045.0
46	38214.0
47	40383.0
48	38890.0
49	37397.0
50	35087.5
51	32778.0
52	28840.0
53	24902.0
54	21123.5
55	17345.0
56	15196.0
57	13047.0
58	11389.5
59	9732.0
60	8380.5
61	7029.0
62	5832.5
63	4636.0
64	3756.5
65	2877.0
66	2457.5
67	2038.0
68	1803.5
69	1569.0
70	1384.0
71	1199.0
72	1038.5
73	878.0
74	710.5
75	412.5
76	282.0
77	234.5
78	187.0
79	140.0
80	93.0
81	71.5
82	50.0
83	35.0
84	20.0
85	18.5
86	17.0
87	13.0
88	9.0
89	5.0
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	443218.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.44460071875035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.97130365093207	38.8278667947296
2	9.65978760263803	9.745682572860202
3	3.862014094945798	5.844532769691805
4	2.0461812981469576	4.128751943327902
5	1.2225661259877556	3.083593003886089
6	0.927565236992269	2.807439481240083
7	0.7624128978766074	2.69217299513476
8	0.6692936585324305	2.7009801094608084
9	0.5699402495226782	2.5875367488644803
>10	3.2758912064792067	24.407991558594123
>50	0.021304669992139893	0.7459333974664015
>100	0.009565362036661796	1.0573786853893679
>500	0.0013043675504538813	0.4195738280750593
>1k	8.695783669692543E-4	0.9505661112793032
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3072	0.6931126443420619	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1262	0.28473572824208404	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTC	720	0.16244827601767076	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCG	658	0.14845967447170466	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGC	535	0.1207080939853526	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2562260558009828E-4	0.0	0.0	0.08054727019209508	0.0
2	2.2562260558009828E-4	0.0	0.0	0.29285814204296756	0.0
3	2.2562260558009828E-4	0.0	0.0	0.40228510574931525	0.0
4	2.2562260558009828E-4	0.0	0.0	0.5974486595761003	0.0
5	2.2562260558009828E-4	0.0	0.0	1.1346560834623143	0.0
6	2.2562260558009828E-4	0.0	0.0	1.5635646566700812	0.0
7	2.2562260558009828E-4	0.0	0.0	1.8952298868728255	0.0
8	2.2562260558009828E-4	0.0	0.0	2.8678889395286293	0.0
9	2.2562260558009828E-4	0.0	0.0	3.3017612100591585	0.0
10	2.2562260558009828E-4	0.0	0.0	4.249376153495571	0.0
11	2.2562260558009828E-4	0.0	0.0	4.824939420330402	0.0
12	2.2562260558009828E-4	0.0	0.0	5.497294784959094	0.0
13	2.2562260558009828E-4	0.0	0.0	5.749766480603225	0.0
14	2.2562260558009828E-4	0.0	0.0	5.883335063106642	0.0
15	2.2562260558009828E-4	0.0	0.0	6.064961260598622	0.0
16	2.2562260558009828E-4	0.0	0.0	6.422121845231917	0.0
17	2.2562260558009828E-4	0.0	0.0	6.837493062104879	0.0
18	4.5124521116019655E-4	0.0	0.0	7.41508693238993	0.0
19	4.5124521116019655E-4	0.0	0.0	7.665528024583839	0.0
20	4.5124521116019655E-4	0.0	0.0	8.005090045981888	0.0
21	4.5124521116019655E-4	0.0	0.0	8.351195122941757	0.0
22	4.5124521116019655E-4	0.0	0.0	8.720764950881959	0.0
23	4.5124521116019655E-4	0.0	0.0	9.11334828459133	0.0
24	4.5124521116019655E-4	0.0	0.0	9.424030612475125	0.0
25	4.5124521116019655E-4	0.0	0.0	9.692521513115443	0.0
26	4.5124521116019655E-4	0.0	0.0	9.96010992333344	0.0
27	4.5124521116019655E-4	0.0	0.0	10.263572327838672	0.0
28	4.5124521116019655E-4	0.0	0.0	10.56116854459882	0.0
29	4.5124521116019655E-4	0.0	0.0	10.874558343749577	0.0
30	4.5124521116019655E-4	0.0	0.0	11.261501112319445	0.0
31	4.5124521116019655E-4	0.0	0.0	11.599935020689593	0.0
32	4.5124521116019655E-4	0.0	0.0	11.953034398422446	0.0
33	4.5124521116019655E-4	0.0	0.0	12.293047665031654	0.0
34	4.5124521116019655E-4	0.0	0.0	12.637573383752464	0.0
35	4.5124521116019655E-4	0.0	0.0	13.003307627397804	0.0
36	4.5124521116019655E-4	0.0	0.0	13.390927263784413	0.0
37	4.5124521116019655E-4	0.0	0.0	13.758240865668814	0.0
38	4.5124521116019655E-4	0.0	0.0	14.152854802828404	0.0
39	4.5124521116019655E-4	0.0	0.0	14.628241632785672	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCACGT	20	7.028591E-4	45.0	14
CTAAGCG	25	3.8869133E-5	45.0	1
ACGGGTC	25	3.8869133E-5	45.0	5
TATTGCG	20	7.028591E-4	45.0	1
CGCAGGA	20	7.028591E-4	45.0	3
TATACGG	40	6.7993824E-9	45.0	2
TCGCGCG	20	7.028591E-4	45.0	1
TCGGGTG	30	2.1624728E-6	44.999996	5
ACGGGAT	140	0.0	43.39286	5
GCGATGC	95	0.0	42.63158	9
CGGTTTA	160	0.0	42.1875	1
CACAACG	60	3.6379788E-12	41.249996	12
CGGTTTT	550	0.0	40.909092	1
TAGGGCG	240	0.0	40.312496	5
GCGGGTA	90	0.0	40.0	5
CGGGCGA	90	0.0	40.0	6
CGTTTTT	1625	0.0	39.32308	1
GGCGATG	390	0.0	38.653847	8
CGATCAT	35	6.239603E-6	38.57143	10
GTTGATC	190	0.0	37.894737	16
>>END_MODULE