FastQCFastQC Report
Sat 18 Jun 2016
SRR3549467_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549467_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295984
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20700.6993621276825774No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8260.2790691388723715No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4250.1435888426401427No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3980.1344667279312395No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3980.1344667279312395No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3950.13345315963025028No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3510.1185874912157414No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA3310.11183036920914644No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGAC351.2078453E-745.00000421
CTACGGG351.2078453E-745.0000043
AGACACG351.2078453E-745.00000424
TGATCGC207.0247904E-445.028
TCGTTGA502.1827873E-1145.024
CTCACGA406.7848305E-945.024
ACTGCGG207.0247904E-445.02
GATCGCC207.0247904E-445.029
CGACGGT406.7848305E-945.028
GCGCGAC1000.045.09
GTCTAAC207.0247904E-445.033
AGGCGAT406.7848305E-945.07
GCCGATC253.8837643E-545.09
CTAACGG207.0247904E-445.02
TACGAAT253.8837643E-545.012
GCCCTCG253.8837643E-545.013
GACGGGT253.8837643E-545.04
CATACGA406.7848305E-945.018
CGGTCTA406.7848305E-945.031
GCTACGA253.8837643E-545.010