##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549467_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	295984
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.649967565814368	31.0	31.0	34.0	30.0	34.0
2	31.787667576625765	31.0	31.0	34.0	30.0	34.0
3	31.400106086815505	31.0	31.0	34.0	28.0	34.0
4	35.44642615817071	37.0	35.0	37.0	33.0	37.0
5	35.32058151791989	37.0	35.0	37.0	33.0	37.0
6	35.37240188658846	37.0	35.0	37.0	33.0	37.0
7	32.142872587707444	37.0	35.0	37.0	10.0	37.0
8	33.722410670847076	37.0	35.0	37.0	19.0	37.0
9	36.69260500567598	39.0	35.0	39.0	32.0	39.0
10	36.5141392777988	38.0	35.0	39.0	32.0	39.0
11	36.58334234282934	38.0	35.0	39.0	32.0	39.0
12	36.5668009081572	38.0	35.0	39.0	32.0	39.0
13	32.790704902967725	38.0	34.0	39.0	8.0	39.0
14	35.37074977025785	39.0	35.0	40.0	18.0	41.0
15	36.775356100329745	39.0	35.0	40.0	31.0	41.0
16	37.39645386237094	39.0	36.0	41.0	32.0	41.0
17	37.52618046921455	39.0	36.0	40.0	33.0	41.0
18	37.47846167360398	39.0	36.0	40.0	33.0	41.0
19	37.47943807773393	39.0	36.0	40.0	33.0	41.0
20	37.41480620574085	39.0	35.0	40.0	33.0	41.0
21	37.33746418725337	39.0	35.0	40.0	32.0	41.0
22	37.325095275420296	39.0	35.0	40.0	32.0	41.0
23	37.2197753932645	39.0	35.0	40.0	32.0	41.0
24	37.11716173847235	39.0	35.0	40.0	32.0	41.0
25	36.9437503378561	39.0	35.0	40.0	32.0	41.0
26	36.8281799016163	39.0	35.0	40.0	31.0	41.0
27	36.80757405805719	39.0	35.0	40.0	31.0	41.0
28	36.67450943294232	38.0	35.0	40.0	31.0	41.0
29	36.62837518244229	38.0	35.0	40.0	31.0	41.0
30	36.47456281420617	38.0	35.0	40.0	31.0	41.0
31	36.36214119682145	38.0	35.0	40.0	30.0	41.0
32	36.19912900697335	38.0	35.0	40.0	30.0	41.0
33	36.00769974052651	38.0	35.0	40.0	30.0	41.0
34	35.8231526028434	38.0	35.0	40.0	29.0	41.0
35	35.72690415698146	38.0	35.0	40.0	28.0	41.0
36	35.498209362668256	38.0	35.0	40.0	27.0	41.0
37	35.40996472782312	38.0	35.0	40.0	26.0	41.0
38	35.28661346559274	38.0	34.0	40.0	26.0	41.0
39	35.12555408400454	38.0	34.0	40.0	25.0	41.0
40	35.014977160927614	38.0	34.0	40.0	24.0	41.0
41	34.95869709173469	38.0	34.0	40.0	24.0	41.0
42	34.85746188983188	38.0	34.0	40.0	24.0	41.0
43	34.7534832963944	38.0	34.0	40.0	23.0	41.0
44	34.52574125628412	38.0	34.0	40.0	23.0	41.0
45	34.4440577869074	38.0	33.0	40.0	23.0	41.0
46	34.30984445105141	38.0	33.0	40.0	22.0	41.0
47	34.1379128601546	38.0	33.0	40.0	21.0	41.0
48	34.046120736256015	37.0	33.0	40.0	21.0	41.0
49	33.963602762311474	37.0	33.0	40.0	20.0	41.0
50	33.78542759068058	37.0	33.0	40.0	20.0	41.0
51	31.9422130925996	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	5.0
10	14.0
11	7.0
12	9.0
13	4.0
14	5.0
15	10.0
16	26.0
17	64.0
18	105.0
19	271.0
20	451.0
21	812.0
22	1176.0
23	1765.0
24	2698.0
25	3698.0
26	4654.0
27	5266.0
28	5616.0
29	6113.0
30	7348.0
31	9239.0
32	12180.0
33	16414.0
34	23022.0
35	28215.0
36	30440.0
37	42987.0
38	50192.0
39	43167.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.18253689388616	23.13908859938375	27.994756473322884	15.683618033407212
2	32.794002378506946	25.933158549110765	27.975836531704417	13.297002540677875
3	31.484472133628845	25.152373101248713	30.10128925887886	13.26186550624358
4	26.62035785718147	27.729877290664362	31.49122925563544	14.158535596518732
5	23.185374885128926	32.895359208605875	29.390102167684738	14.529163738580465
6	20.976809557273366	42.68778042056328	26.624412130385426	9.710997891777934
7	75.20102437969621	14.351113573706687	8.531880101627115	1.9159819449699984
8	82.30884101843343	5.723958051786584	9.06130061084383	2.9059003189361587
9	75.30373263419644	8.369371317368508	11.897264717011732	4.4296313314233196
10	31.841586031677387	36.57258500459484	18.56113843991567	13.0246905238121
11	29.066098167468514	28.16064381858479	28.10253256932807	14.670725444618629
12	27.563314233201798	24.793232066598193	31.11789826477107	16.52555543542894
13	19.849383750473	36.90402183901833	28.834328882642307	14.412265527866372
14	17.062746634953243	34.41740094059138	31.577720417319853	16.94213200713552
15	17.132682847721497	27.70386237093897	38.934199145899775	16.229255635439756
16	19.289894048326936	26.141277906913885	36.624276987945294	17.944551056813882
17	18.21145737607438	25.529082653116387	32.91664414292664	23.34281582788259
18	19.83418022595816	26.40412995297043	34.70424076977134	19.057449051300072
19	24.226985242445537	26.517649602681225	30.94626736580356	18.30909778906968
20	24.461119519974055	26.17540137304719	30.8229498891832	18.540529217795555
21	21.609614033190983	28.329234012649334	31.979431320611923	18.08172063354776
22	19.620993026650087	26.297367425266234	30.434077517703663	23.64756203038002
23	18.7533785610033	29.348883723444512	30.92903670468674	20.968701010865452
24	17.51851451429807	27.89204821882264	35.75227039299422	18.837166873885074
25	18.26551435212714	29.41881993621277	32.751432509865396	19.564233201794693
26	16.94280771933618	31.780771933618034	31.053367749608086	20.2230525974377
27	19.04224552678523	30.155346234931617	31.22026866317098	19.582139575112166
28	17.47830963835883	30.2323774258068	34.26300070274068	18.02631223309368
29	18.37734472133629	27.9086031677388	33.346397102546085	20.367655008378833
30	18.567895561922263	30.23035028920482	32.71291691442781	18.488837234445104
31	22.35154602951511	29.624912157413913	29.21238985891129	18.811151954159687
32	22.33870749770258	29.569503756959836	29.959051840640033	18.132736904697552
33	20.961268176658198	29.540785988431807	29.571530893561814	19.92641494134818
34	18.051989296718745	30.826328450186498	30.645237580409752	20.47644467268501
35	20.278460997891777	26.979161035731664	32.051056813881836	20.69132115249473
36	21.90422455267852	30.522257959889725	29.13434509973512	18.43917238769663
37	19.84701875777069	31.37906103032596	30.556719282123357	18.217200929779988
38	19.36827666360344	31.843613168279365	28.363357478782635	20.424752689334557
39	20.752473106654413	28.90967079301584	28.647494459159955	21.69036164116979
40	21.33122060651927	28.378898859397804	30.174941888750745	20.11493864533218
41	18.0401643332072	27.850154062381748	30.453673171522784	23.656008432888264
42	19.625047299854046	28.77216336018163	29.24448618844262	22.358303151521707
43	19.438212876371697	27.600140548137738	30.708078815071083	22.253567760419482
44	18.22260662738526	30.5935455970593	30.40772474187794	20.776123033677496
45	18.172266068436134	31.63819665927888	29.487066868479378	20.702470403805613
46	19.92810422184983	31.70745715984648	28.249499972971513	20.11493864533218
47	20.268325314881885	28.29173198551273	29.98168819936213	21.458254500243257
48	19.031434131574677	28.65560300556787	31.290204875939242	21.02275798691821
49	19.310165414346724	28.21334937023623	31.528393426671713	20.948091788745337
50	18.94899724309422	29.302935293799663	31.11688469647008	20.631182766636034
51	19.588558841018433	27.900494621330886	29.300232444997025	23.210714092653657
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	348.0
1	501.5
2	655.0
3	2284.5
4	3914.0
5	3476.0
6	3038.0
7	2395.5
8	1753.0
9	1745.5
10	1738.0
11	1751.5
12	1765.0
13	1772.5
14	1780.0
15	1735.0
16	1690.0
17	1588.0
18	1486.0
19	1456.5
20	1427.0
21	1667.5
22	1908.0
23	2079.0
24	2250.0
25	2749.0
26	3960.5
27	4673.0
28	5256.0
29	5839.0
30	7087.5
31	8336.0
32	9206.5
33	10077.0
34	10780.5
35	11484.0
36	11983.0
37	12482.0
38	14164.0
39	15846.0
40	18384.0
41	20922.0
42	22614.5
43	24307.0
44	25635.5
45	26964.0
46	27661.5
47	28359.0
48	27296.5
49	26234.0
50	24234.5
51	22235.0
52	19370.5
53	16506.0
54	13842.0
55	11178.0
56	9242.5
57	7307.0
58	6239.0
59	5171.0
60	4448.0
61	3725.0
62	3032.0
63	2339.0
64	1940.0
65	1541.0
66	1301.0
67	1061.0
68	914.0
69	767.0
70	682.0
71	597.0
72	524.5
73	452.0
74	353.5
75	197.0
76	139.0
77	115.5
78	92.0
79	66.5
80	41.0
81	36.0
82	31.0
83	21.5
84	12.0
85	9.0
86	6.0
87	5.0
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	295984.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.95016331325132
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.54137816361104	37.18121746479729
2	8.727303072264906	8.369512151986843
3	3.66665714949005	5.274504273952455
4	1.969343930571087	3.777214523633756
5	1.2723138556800255	3.0503828582784847
6	0.9510591865012321	2.7362065987981214
7	0.7454863488947236	2.502233452411102
8	0.6640361079606065	2.54725118580855
9	0.5291544676620945	2.28357388281006
>10	3.8837097039785147	28.616705299969595
>50	0.03210800867244464	1.0228194558811792
>100	0.01605400433622232	1.669110152643796
>500	6.980001885314054E-4	0.2764557822506067
>1k	6.980001885314054E-4	0.6928129167781547
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2070	0.6993621276825774	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	826	0.2790691388723715	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	425	0.1435888426401427	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	398	0.1344667279312395	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	398	0.1344667279312395	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	395	0.13345315963025028	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	351	0.1185874912157414	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	331	0.11183036920914644	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.07263906157089572	0.0
2	0.0	0.0	0.0	0.2881912535812747	0.0
3	0.0	0.0	0.0	0.3990080544894319	0.0
4	0.0	0.0	0.0	0.5703010973566138	0.0
5	0.0	0.0	0.0	1.0351910914103466	0.0
6	0.0	0.0	0.0	1.4115627871776852	0.0
7	0.0	0.0	0.0	1.7004297529596195	0.0
8	0.0	0.0	0.0	2.600140548137737	0.0
9	0.0	0.0	0.0	3.0035407319314555	0.0
10	0.0	0.0	0.0	3.8373695875452727	0.0
11	0.0	0.0	0.0	4.432334180225959	0.0
12	0.0	0.0	0.0	5.100951402778529	0.0
13	0.0	0.0	0.0	5.371574139142656	0.0
14	0.0	0.0	0.0	5.491175198659387	0.0
15	0.0	0.0	0.0	5.703686685766798	0.0
16	0.0	0.0	0.0	6.111141142764474	0.0
17	0.0	0.0	0.0	6.578058273420185	0.0
18	3.3785610032974753E-4	0.0	0.0	7.261541164387264	0.0
19	3.3785610032974753E-4	0.0	0.0	7.553786691172496	0.0
20	3.3785610032974753E-4	0.0	0.0	7.895021352505541	0.0
21	3.3785610032974753E-4	0.0	0.0	8.253486674955402	0.0
22	3.3785610032974753E-4	0.0	0.0	8.65992756365209	0.0
23	3.3785610032974753E-4	0.0	0.0	9.106573328288016	0.0
24	3.3785610032974753E-4	0.0	0.0	9.453551543326666	0.0
25	3.3785610032974753E-4	0.0	0.0	9.733296394399698	0.0
26	3.3785610032974753E-4	0.0	0.0	10.017433374777015	0.0
27	3.3785610032974753E-4	0.0	0.0	10.313057462565544	0.0
28	3.3785610032974753E-4	0.0	0.0	10.61138439915671	0.0
29	3.3785610032974753E-4	0.0	0.0	10.945524082382832	0.0
30	6.757122006594951E-4	0.0	0.0	11.338112870965999	0.0
31	6.757122006594951E-4	0.0	0.0	11.708403156927401	0.0
32	6.757122006594951E-4	0.0	0.0	12.088153413698038	0.0
33	6.757122006594951E-4	0.0	0.0	12.461484404562409	0.0
34	6.757122006594951E-4	0.0	0.0	12.84697821503865	0.0
35	6.757122006594951E-4	0.0	0.0	13.280109735661387	0.0
36	6.757122006594951E-4	0.0	0.0	13.685874912157415	0.0
37	6.757122006594951E-4	0.0	0.0	14.086910103248824	0.0
38	6.757122006594951E-4	0.0	0.0	14.462606086815503	0.0
39	6.757122006594951E-4	0.0	0.0	14.872425536515488	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	35	1.2078453E-7	45.000004	21
CTACGGG	35	1.2078453E-7	45.000004	3
AGACACG	35	1.2078453E-7	45.000004	24
TGATCGC	20	7.0247904E-4	45.0	28
TCGTTGA	50	2.1827873E-11	45.0	24
CTCACGA	40	6.7848305E-9	45.0	24
ACTGCGG	20	7.0247904E-4	45.0	2
GATCGCC	20	7.0247904E-4	45.0	29
CGACGGT	40	6.7848305E-9	45.0	28
GCGCGAC	100	0.0	45.0	9
GTCTAAC	20	7.0247904E-4	45.0	33
AGGCGAT	40	6.7848305E-9	45.0	7
GCCGATC	25	3.8837643E-5	45.0	9
CTAACGG	20	7.0247904E-4	45.0	2
TACGAAT	25	3.8837643E-5	45.0	12
GCCCTCG	25	3.8837643E-5	45.0	13
GACGGGT	25	3.8837643E-5	45.0	4
CATACGA	40	6.7848305E-9	45.0	18
CGGTCTA	40	6.7848305E-9	45.0	31
GCTACGA	25	3.8837643E-5	45.0	10
>>END_MODULE