##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549466_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	366476
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58151147687707	31.0	31.0	34.0	30.0	34.0
2	31.676448116657024	31.0	31.0	34.0	30.0	34.0
3	31.194080922079483	31.0	31.0	34.0	28.0	34.0
4	35.32806786801864	37.0	35.0	37.0	33.0	37.0
5	35.23174778157369	37.0	35.0	37.0	33.0	37.0
6	35.29343258494417	37.0	35.0	37.0	33.0	37.0
7	32.09773354871806	36.0	35.0	37.0	11.0	37.0
8	33.69712341326581	37.0	35.0	37.0	19.0	37.0
9	36.681310645171855	39.0	35.0	39.0	32.0	39.0
10	36.40756011307698	38.0	35.0	39.0	32.0	39.0
11	36.459686309608266	38.0	35.0	39.0	32.0	39.0
12	36.44845228609786	38.0	35.0	39.0	32.0	39.0
13	32.78311540182713	37.0	34.0	39.0	10.0	39.0
14	35.34225160719938	39.0	34.0	40.0	18.0	41.0
15	36.71743579388555	39.0	35.0	40.0	30.0	41.0
16	37.30770364225761	39.0	36.0	40.0	32.0	41.0
17	37.4430822209367	39.0	36.0	40.0	32.0	41.0
18	37.367505648391706	39.0	36.0	40.0	32.0	41.0
19	37.364468614588674	39.0	36.0	40.0	32.0	41.0
20	37.254554186358725	39.0	35.0	40.0	32.0	41.0
21	37.20166122747465	39.0	35.0	40.0	32.0	41.0
22	37.19749997271308	39.0	35.0	40.0	32.0	41.0
23	37.11331164933038	39.0	35.0	40.0	32.0	41.0
24	37.00359641559065	39.0	35.0	40.0	32.0	41.0
25	36.82406760606425	38.0	35.0	40.0	31.0	41.0
26	36.714245953350286	38.0	35.0	40.0	31.0	41.0
27	36.69121306715856	38.0	35.0	40.0	31.0	41.0
28	36.52001768192187	38.0	35.0	40.0	31.0	41.0
29	36.44712068457416	38.0	35.0	40.0	31.0	41.0
30	36.28377028782239	38.0	35.0	40.0	30.0	41.0
31	36.203593141160674	38.0	35.0	40.0	30.0	41.0
32	36.01842412600006	38.0	35.0	40.0	30.0	41.0
33	35.79063567600607	38.0	35.0	40.0	29.0	41.0
34	35.56429343258495	38.0	35.0	40.0	27.0	41.0
35	35.35243781311737	38.0	34.0	40.0	26.0	41.0
36	35.13483011165806	38.0	34.0	40.0	25.0	41.0
37	35.01150962136675	38.0	34.0	40.0	24.0	41.0
38	34.90564730023249	38.0	34.0	40.0	24.0	41.0
39	34.77469465940471	38.0	34.0	40.0	23.0	41.0
40	34.64289885285803	38.0	34.0	40.0	23.0	41.0
41	34.588295004311334	38.0	34.0	40.0	23.0	41.0
42	34.55846767591875	38.0	34.0	40.0	23.0	41.0
43	34.54887359608815	38.0	34.0	40.0	23.0	41.0
44	34.3632079590478	38.0	33.0	40.0	22.0	41.0
45	34.35790611117781	38.0	33.0	40.0	22.0	41.0
46	34.252223883692245	38.0	33.0	40.0	22.0	41.0
47	34.106225237123304	38.0	33.0	40.0	21.0	41.0
48	33.99539942588328	37.0	33.0	40.0	20.0	41.0
49	33.89383479409293	37.0	33.0	40.0	20.0	41.0
50	33.684994378895205	37.0	33.0	40.0	19.0	41.0
51	31.870567240419565	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	7.0
10	21.0
11	5.0
12	6.0
13	4.0
14	10.0
15	15.0
16	29.0
17	77.0
18	175.0
19	375.0
20	689.0
21	1160.0
22	1799.0
23	2651.0
24	3861.0
25	5514.0
26	6597.0
27	6960.0
28	7047.0
29	7682.0
30	9037.0
31	11396.0
32	14587.0
33	20352.0
34	28690.0
35	35364.0
36	37613.0
37	52446.0
38	61123.0
39	51171.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.56972898634562	23.27546687914079	27.084993287418545	16.069810847095034
2	32.852628821532655	27.25908381449263	27.258810945327934	12.629476418646787
3	29.920922516071997	25.923934991650206	31.133825953131993	13.021316539145811
4	25.073401805302392	27.280367609338676	33.87261375915476	13.773616826204172
5	22.694255558344885	32.66243901374169	30.574444165511522	14.068861262401903
6	20.268721553389582	41.75771401128587	28.722208275576026	9.251356159748523
7	73.86650148986564	14.143627413527762	9.974186577020049	2.0156845195865487
8	81.59497484146301	5.674041410624434	10.01156965258298	2.7194140953295713
9	75.14789508726356	7.7361136882087775	12.569445202414348	4.546546022113318
10	33.825134524498196	31.381318285508463	21.397854156888855	13.395693033104486
11	28.79479147338434	27.481745052882044	28.022571737303398	15.700891736430217
12	25.695816369966927	24.77624728495181	33.28458070924153	16.243355635839727
13	19.785470262718434	36.91919798295113	29.63932153810891	13.656010216221526
14	18.148255274560952	33.997860705748806	31.846287342145192	16.00759667754505
15	17.470448269463756	27.088267717394864	40.484506488828735	14.956777524312642
16	18.192732948405897	26.766827841386615	36.54891452646285	18.491524683744636
17	18.283325511083945	26.089020836289418	32.51727261812506	23.110381034501575
18	20.748425544919723	27.294011067573322	34.134568157259956	17.822995230247002
19	23.458562088649735	28.536111505255462	30.561073576441565	17.444252829653237
20	23.69432104694441	26.920726050273412	31.46918215653958	17.91577074624259
21	21.312718977504666	29.127418985145002	31.899496829260304	17.660365208090028
22	19.752998832119975	27.245440356257983	30.337866599722762	22.66369421189928
23	18.310339558388545	29.772208821314354	30.736255580174415	21.181196040122682
24	18.207467883299316	27.35567949879392	35.117988626813215	19.31886399109355
25	17.14191379517349	31.085528110981347	32.467883299315645	19.304674794529518
26	17.193213198135755	30.902978639801788	31.727316386339076	20.176491775723377
27	18.27322935199031	31.116362326591645	30.850042021851365	19.760366299566684
28	16.41799190124319	29.45513485194119	35.05795741058078	19.068915836234844
29	17.603335552669204	27.397701350156627	34.016688678112615	20.982274419061547
30	17.735131359215885	30.111658062192344	33.34624914046213	18.806961438129647
31	20.80900249948155	29.27149390410286	30.23226623298661	19.687237363428984
32	20.712133946015566	28.73639747214006	31.57396391578166	18.977504666062718
33	19.927089359194053	29.267946604961853	30.287112935089883	20.51785110075421
34	17.73676857420404	27.63100448596907	32.25859264999618	22.37363428983071
35	18.428491906700575	27.92897761381373	31.992272345255895	21.650258134229798
36	21.29034370599985	29.056473002324847	30.587814754581473	19.065368537093835
37	18.103777600716008	31.16247721542475	30.85931957345092	19.874425610408323
38	17.614523188421614	31.447625492528843	30.027614359466924	20.91023695958262
39	20.179766205699693	29.51571180650302	29.694441109376875	20.610080878420415
40	19.970748425544922	28.412774642814263	31.55049716761807	20.065979764022746
41	18.01209356137919	28.552210785972342	30.071000556653093	23.364695095995373
42	19.453388489287157	28.692192667459803	30.152315567731584	21.702103275521452
43	19.907715648500858	26.873246815616845	31.162204346260054	22.056833189622242
44	18.18754843427673	28.95605714971785	30.913620537224812	21.942773878780603
45	17.570318383741363	31.163841561248212	29.548183237101473	21.71765681790895
46	19.382988244796383	30.985112258374354	29.291686222290135	20.340213274539124
47	18.373918073761995	28.6616313210142	31.2874512928541	21.676999312369706
48	18.72100765125138	28.122714720745694	31.10954059747432	22.0467370305286
49	19.71588862572174	27.126196531287178	32.288881127277094	20.86903371571399
50	18.374190942926685	29.082395572970672	31.063152839476526	21.480260644626114
51	18.050840982765585	28.0610462895251	29.756382409762168	24.13173031794715
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	386.0
1	470.0
2	554.0
3	2931.5
4	5309.0
5	4702.5
6	4096.0
7	3246.0
8	2396.0
9	2493.0
10	2590.0
11	2596.0
12	2602.0
13	2680.0
14	2758.0
15	2640.0
16	2522.0
17	2433.5
18	2345.0
19	2305.5
20	2266.0
21	2317.5
22	2369.0
23	2519.5
24	2670.0
25	3477.5
26	4532.0
27	4779.0
28	5868.0
29	6957.0
30	8077.0
31	9197.0
32	10636.0
33	12075.0
34	12971.0
35	13867.0
36	15515.0
37	17163.0
38	18569.5
39	19976.0
40	22646.0
41	25316.0
42	27487.0
43	29658.0
44	30658.0
45	31658.0
46	34529.5
47	37401.0
48	35677.0
49	33953.0
50	31356.0
51	28759.0
52	24517.5
53	20276.0
54	16883.0
55	13490.0
56	11235.0
57	8980.0
58	7338.0
59	5696.0
60	4703.5
61	3711.0
62	3112.5
63	2514.0
64	1950.0
65	1386.0
66	1147.0
67	908.0
68	766.5
69	625.0
70	495.0
71	365.0
72	302.0
73	239.0
74	204.5
75	130.5
76	91.0
77	67.5
78	44.0
79	39.0
80	34.0
81	25.5
82	17.0
83	13.5
84	10.0
85	7.5
86	5.0
87	4.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	366476.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.506061186910784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.86999637514884	37.77166808797491
2	9.022557075966404	8.752974111784424
3	3.6941768292830446	5.375699019494143
4	1.9712723925553832	3.8247463715743772
5	1.2189791490143052	2.9563938593828163
6	0.8892612502598188	2.588073636975093
7	0.6928930580165108	2.352665914769421
8	0.5784507022178075	2.244669212431078
9	0.47536165372923983	2.0752129315531445
>10	3.5409456853823476	27.992207372040106
>50	0.03055205498127449	0.9632739013999159
>100	0.012220821992509786	1.2796049672022927
>500	0.0022219676350017795	0.7226575114837964
>1k	0.0011109838175008897	1.1001531019345046
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2901	0.7915934467741408	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1182	0.3225313526670232	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCGTCTTC	743	0.20274178936683437	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGCCG	732	0.19974022855521234	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTTCAGTCGTATGC	618	0.16863314378022026	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	589	0.1607199380041258	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	415	0.11324070334755891	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.186074940787391E-4	0.0	0.0	0.087863871031118	0.0
2	8.186074940787391E-4	0.0	0.0	0.35882295157118066	0.0
3	8.186074940787391E-4	0.0	0.0	0.49252884227070803	0.0
4	8.186074940787391E-4	0.0	0.0	0.756393324528755	0.0
5	8.186074940787391E-4	0.0	0.0	1.4489352645193683	0.0
6	8.186074940787391E-4	0.0	0.0	1.9324594243552102	0.0
7	8.186074940787391E-4	0.0	0.0	2.2860978617972254	0.0
8	8.186074940787391E-4	0.0	0.0	3.155731889673539	0.0
9	8.186074940787391E-4	0.0	0.0	3.517829271221035	0.0
10	8.186074940787391E-4	0.0	0.0	4.4859690675514905	0.0
11	8.186074940787391E-4	0.0	0.0	5.156954343531363	0.0
12	8.186074940787391E-4	0.0	0.0	5.984020781715583	0.0
13	8.186074940787391E-4	0.0	0.0	6.312009517676464	0.0
14	8.186074940787391E-4	0.0	0.0	6.460996081598795	0.0
15	8.186074940787391E-4	0.0	0.0	6.674925506718039	0.0
16	8.186074940787391E-4	0.0	0.0	7.083683515428023	0.0
17	8.186074940787391E-4	0.0	0.0	7.569663497746101	0.0
18	8.186074940787391E-4	0.0	0.0	8.260295353583864	0.0
19	8.186074940787391E-4	0.0	0.0	8.557995612263833	0.0
20	8.186074940787391E-4	0.0	0.0	8.917364302164398	0.0
21	8.186074940787391E-4	0.0	0.0	9.342494460755956	0.0
22	8.186074940787391E-4	0.0	0.0	9.816195330662854	0.0
23	8.186074940787391E-4	0.0	0.0	10.312817210403956	0.0
24	8.186074940787391E-4	0.0	0.0	10.70847749920868	0.0
25	8.186074940787391E-4	0.0	0.0	11.037830580993026	0.0
26	8.186074940787391E-4	0.0	0.0	11.352721597048648	0.0
27	8.186074940787391E-4	0.0	0.0	11.683711893821151	0.0
28	8.186074940787391E-4	0.0	0.0	12.035713116275009	0.0
29	8.186074940787391E-4	0.0	0.0	12.409271002739606	0.0
30	8.186074940787391E-4	0.0	0.0	12.835219768825244	0.0
31	0.001091476658771652	0.0	0.0	13.226241281830188	0.0
32	0.001091476658771652	0.0	0.0	13.597889084141936	0.0
33	0.001091476658771652	0.0	0.0	14.011558737816392	0.0
34	0.001091476658771652	0.0	0.0	14.423318307337997	0.0
35	0.001091476658771652	0.0	0.0	14.852268634235257	0.0
36	0.001091476658771652	0.0	0.0	15.28722208275576	0.0
37	0.001091476658771652	0.0	0.0	15.700073128936138	0.0
38	0.001091476658771652	0.0	0.0	16.13748239993888	0.0
39	0.001091476658771652	0.0	0.0	16.55824665189535	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACGA	30	2.1614396E-6	45.000004	24
CGGTTTA	105	0.0	45.000004	1
ACTCCCG	30	2.1614396E-6	45.000004	13
GTTTGCG	20	7.026992E-4	45.0	1
CGTGGGT	20	7.026992E-4	45.0	4
CGGCGAA	25	3.8855876E-5	45.0	35
TCGCAGG	20	7.026992E-4	45.0	2
GACACGG	20	7.026992E-4	45.0	2
CGCATGG	45	3.8380676E-10	45.0	2
AAGACCG	20	7.026992E-4	45.0	24
TTCGTAA	20	7.026992E-4	45.0	1
GTAGCGG	20	7.026992E-4	45.0	2
AACGCGG	20	7.026992E-4	45.0	2
TTAACGG	20	7.026992E-4	45.0	2
CCGAATC	20	7.026992E-4	45.0	34
GGCGAAC	20	7.026992E-4	45.0	8
CGGCTAC	95	0.0	42.63158	25
CGGTTTT	630	0.0	42.499996	1
ATGGTCC	85	0.0	42.352943	11
CGTTTTT	1650	0.0	41.454548	1
>>END_MODULE