SRR3549463_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549463_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	351360
Sequences flagged as poor quality	0
Sequence length	51
%GC	37

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9027	2.5691598360655736	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3575	1.0174749544626593	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG	1820	0.5179872495446266	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC	1344	0.3825136612021858	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1019	0.29001593806921677	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	957	0.27237021857923494	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	920	0.26183970856102007	No Hit
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	836	0.2379326047358834	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	834	0.23736338797814208	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	813	0.23138661202185795	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC	757	0.2154485428051002	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	751	0.21374089253187611	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	503	0.14315801457194902	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCT	484	0.1377504553734062	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	474	0.13490437158469945	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTC	459	0.13063524590163936	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGGTAC	75	0.0	45.000004	6
GCGCAAG	30	2.1611831E-6	45.000004	1
TCGTCCC	30	2.1611831E-6	45.000004	38
TGATCGT	20	7.026594E-4	45.0	28
CTTCGTC	20	7.026594E-4	45.0	31
TAGCGAG	20	7.026594E-4	45.0	1
CGACCCG	20	7.026594E-4	45.0	32
ATTAACG	20	7.026594E-4	45.0	1
TTCGTCA	20	7.026594E-4	45.0	32
CGACAGG	35	1.2087003E-7	45.0	2
CCGCTGA	20	7.026594E-4	45.0	36
TATTGCG	20	7.026594E-4	45.0	1
GGGCGCA	20	7.026594E-4	45.0	7
GCGATGA	40	6.7921064E-9	45.0	9
TAGGCGA	20	7.026594E-4	45.0	6
CACGTAG	20	7.026594E-4	45.0	1
CGTCAGT	20	7.026594E-4	45.0	34
CGGACGG	20	7.026594E-4	45.0	2
TGATCGG	25	3.88526E-5	44.999996	28
CGGTTTA	250	0.0	44.999996	1