FastQCFastQC Report
Sat 18 Jun 2016
SRR3549462_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549462_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261551
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39001.4911049852610008No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14990.5731195827964718No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG7140.27298691268624475No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6670.2550171859407917No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5180.19804932881158935No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4550.1739622482804501No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.1628745445438939No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3940.15063983697252162No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA3890.14872816391449467No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3670.14031680245917622No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC2980.11393571425840467No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCTCA302.1590513E-645.0000047
AATGGGC600.045.0000044
CACTGGG253.8825172E-545.0000043
TCAATCG253.8825172E-545.00000430
TAGGGCG600.045.0000045
CAATCGC253.8825172E-545.00000431
ATCGCTA253.8825172E-545.00000433
ATACATG253.8825172E-545.00000442
AATCGCT302.1590513E-645.00000432
ATGGGCG453.8198777E-1045.05
ATCTAAT207.023287E-445.022
CGGTTTA1400.045.01
TCTTAGG453.8198777E-1045.02
ATAGCGG351.2071177E-745.02
TAAACGG207.023287E-445.02
TACGGTT351.2071177E-745.033
CCTCGTG551.8189894E-1245.015
GGGACGT207.023287E-445.07
CTACGGG551.8189894E-1245.03
ACTACGG207.023287E-445.02