##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549462_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	261551
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.598208380010018	31.0	31.0	34.0	30.0	34.0
2	31.66641687472042	31.0	31.0	34.0	30.0	34.0
3	31.01446371835703	31.0	31.0	34.0	27.0	34.0
4	35.29652725472279	37.0	35.0	37.0	33.0	37.0
5	35.26511464303329	37.0	35.0	37.0	33.0	37.0
6	35.34699924680081	37.0	35.0	37.0	33.0	37.0
7	31.98727972747189	36.0	35.0	37.0	10.0	37.0
8	33.59794074578189	37.0	35.0	37.0	17.0	37.0
9	36.67755045861037	39.0	35.0	39.0	32.0	39.0
10	36.655115063601365	38.0	35.0	39.0	32.0	39.0
11	36.62517061682043	38.0	35.0	39.0	32.0	39.0
12	36.52181410126514	38.0	35.0	39.0	32.0	39.0
13	32.79117648183337	37.0	34.0	39.0	11.0	39.0
14	35.34119158405053	39.0	34.0	40.0	18.0	41.0
15	36.691937710045075	39.0	35.0	40.0	30.0	41.0
16	37.29240568760968	39.0	35.0	41.0	32.0	41.0
17	37.42013221130869	39.0	36.0	40.0	32.0	41.0
18	37.336293877675864	39.0	36.0	40.0	32.0	41.0
19	37.264089986274186	39.0	36.0	40.0	32.0	41.0
20	37.12779152058298	39.0	35.0	40.0	32.0	41.0
21	37.10843774254352	39.0	35.0	40.0	32.0	41.0
22	37.13493353112777	39.0	35.0	40.0	33.0	41.0
23	37.051053140687664	38.0	35.0	40.0	32.0	41.0
24	36.9046648645962	38.0	35.0	40.0	32.0	41.0
25	36.759240071725976	38.0	35.0	40.0	32.0	41.0
26	36.60829054371805	38.0	35.0	40.0	32.0	41.0
27	36.62164166835531	38.0	35.0	40.0	32.0	41.0
28	36.517096092157935	38.0	35.0	40.0	31.0	41.0
29	36.492454626439965	38.0	35.0	40.0	31.0	41.0
30	36.360744176088026	38.0	35.0	40.0	31.0	41.0
31	36.204969585281646	38.0	35.0	40.0	31.0	41.0
32	35.95980133893581	38.0	35.0	40.0	30.0	41.0
33	35.6702478675287	38.0	35.0	40.0	29.0	41.0
34	35.391613872629044	38.0	34.0	40.0	27.0	41.0
35	35.14686619435598	38.0	34.0	40.0	25.0	41.0
36	34.84657294370888	38.0	34.0	40.0	24.0	41.0
37	34.675829952858145	38.0	34.0	40.0	23.0	41.0
38	34.519149229022254	38.0	34.0	40.0	22.0	41.0
39	34.09819499829861	38.0	33.0	40.0	18.0	41.0
40	33.79579890728768	38.0	33.0	40.0	16.0	41.0
41	33.65594090636243	38.0	33.0	40.0	15.0	41.0
42	33.40314890786118	37.0	33.0	40.0	15.0	41.0
43	33.25708179284346	37.0	33.0	40.0	14.0	41.0
44	32.76124350509078	37.0	32.0	40.0	10.0	41.0
45	32.500430126438054	37.0	31.0	40.0	10.0	41.0
46	32.02337976149967	36.0	30.0	40.0	9.0	41.0
47	31.792327309014304	36.0	30.0	40.0	9.0	41.0
48	31.727559825808353	36.0	30.0	40.0	9.0	41.0
49	31.523569017132413	36.0	29.0	40.0	8.0	41.0
50	31.330337104427052	36.0	28.0	40.0	8.0	41.0
51	29.669406731383173	34.0	24.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	6.0
10	21.0
11	9.0
12	5.0
13	7.0
14	10.0
15	5.0
16	23.0
17	58.0
18	115.0
19	239.0
20	402.0
21	706.0
22	1206.0
23	1912.0
24	2994.0
25	4402.0
26	5812.0
27	6710.0
28	7304.0
29	7823.0
30	8740.0
31	10195.0
32	12040.0
33	15972.0
34	21630.0
35	24690.0
36	23241.0
37	32829.0
38	38797.0
39	33645.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.59344831409553	22.834552343520002	25.36637214157086	18.20562720081361
2	34.25029917683358	29.65693115300649	24.708374274997993	11.384395395161938
3	29.941770438652497	26.775275185336707	31.122037384678325	12.160916991332474
4	25.463867467530232	24.904129596139953	36.844057181964516	12.787945754365303
5	23.89094287538568	30.401336641802175	31.157212168946018	14.550508313866128
6	20.296232857071853	40.64561022515685	29.905066315938384	9.153090601832913
7	68.1622322223964	14.937813275422386	14.501187148969036	2.3987673532121843
8	74.02456882214177	8.493945731425228	14.129940241100206	3.351545205332803
9	67.52411575562701	8.83689987803526	16.304659511911634	7.334324854426097
10	37.06810526436527	27.877928205206633	23.200446566826354	11.853519963601746
11	31.466138535123168	23.800329572435203	30.082087241111676	14.651444651329951
12	29.10636931229473	22.309606921785807	32.88039426345149	15.703629502467969
13	18.123425259318452	36.196764684516594	31.344938463244258	14.334871592920692
14	15.315177537076899	33.6339757829257	33.28184560563714	17.769001074360258
15	16.714139880941	25.239819385129476	43.28677772212685	14.75926301180267
16	16.115786213778573	26.298886259276394	39.532251836161976	18.05307569078306
17	16.02440824160489	25.576656177953822	34.83030078263895	23.56863479780234
18	17.80111718173511	27.791520582983814	36.82302877832622	17.584333456954855
19	23.30520625040623	26.8249786848454	32.12031305557998	17.74950200916838
20	22.94657638472038	26.493494576583533	34.545079162381334	16.014849876314752
21	19.188991821862658	29.954769815447087	34.085512959231664	16.7707254034586
22	18.135277632278218	25.881376863403315	32.73090143031378	23.25244407400469
23	18.770717756766366	28.435754403538887	33.52080473789051	19.27272310180424
24	17.513219219196255	25.47074949053913	38.37301329377444	18.64301799649017
25	16.649907666191297	30.240373770316303	34.386792633176704	18.722925930315693
26	15.713952536981315	30.504184652324025	33.88861063425489	19.893252176439777
27	18.732101960994225	30.7798479072915	33.101766003571	17.386284128143267
28	14.22667089783637	29.564023842386376	38.25716590645801	17.95213935331924
29	17.36143237838892	25.90049359398358	37.15642455964611	19.58164946798139
30	16.581469770713934	30.03888343000027	35.98533364429882	17.394313154986982
31	22.6426203684941	27.758639806385755	32.52711708232811	17.071622742792037
32	23.214592947455753	27.54950277383761	32.717519718907596	16.518384559799046
33	20.050009367197987	27.845429763220174	32.84139613306774	19.263164736514103
34	18.96762008174314	26.432321038726673	34.91441439719213	19.685644482338052
35	20.84794170161842	25.076562505973975	34.35391185657864	19.72158393582896
36	20.638039999847066	27.933366723889414	33.11247137269596	18.316121903567563
37	19.367159750870766	29.6011102997121	34.337089133668	16.694640815749125
38	18.48243745961591	29.188188919178288	32.14248846305309	20.186885158152712
39	21.81563060359165	26.642222740498028	31.136948434530932	20.405198221379386
40	21.296420201031538	25.87755351728726	33.099471995901375	19.72655428577983
41	17.66844707150804	26.089749226728248	32.350096157154816	23.891707544608888
42	19.136229645461114	25.535364039900436	32.19716231251267	23.13124400212578
43	20.08518415146568	24.77222415513609	31.96470286865659	23.17788882474164
44	17.09265114643033	28.051890453487083	32.451032494618644	22.404425905463942
45	17.92040558055599	30.26484318545905	29.188188919178288	22.626562314806673
46	18.70648554201666	29.697076287225055	30.373426215154982	21.223011955603305
47	19.198167852541186	25.61106629299831	33.535715787743115	21.65505006671739
48	18.716043907306798	25.46922015209271	34.71865907605018	21.096076864550316
49	18.226655604451906	25.73417803793524	34.561519550680366	21.47764680693249
50	17.203528183795893	27.596147596453463	34.13712813179839	21.063196087952253
51	17.57095174554867	25.725384341868317	31.841973458331264	24.861690454251754
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	330.0
1	458.0
2	586.0
3	3652.5
4	6719.0
5	5752.0
6	4785.0
7	3618.0
8	2451.0
9	2286.5
10	2122.0
11	2120.0
12	2118.0
13	2083.5
14	2049.0
15	1929.5
16	1810.0
17	1743.5
18	1677.0
19	1710.5
20	1744.0
21	1804.0
22	1864.0
23	1960.0
24	2056.0
25	2564.0
26	3883.5
27	4695.0
28	5287.0
29	5879.0
30	6354.5
31	6830.0
32	7391.0
33	7952.0
34	8931.5
35	9911.0
36	10017.5
37	10124.0
38	11259.5
39	12395.0
40	14533.5
41	16672.0
42	18163.0
43	19654.0
44	20547.5
45	21441.0
46	22278.0
47	23115.0
48	23020.0
49	22925.0
50	21626.5
51	20328.0
52	17850.0
53	15372.0
54	13116.5
55	10861.0
56	8900.0
57	6939.0
58	5825.5
59	4712.0
60	4058.0
61	3404.0
62	2718.5
63	2033.0
64	1565.5
65	1098.0
66	869.5
67	641.0
68	543.5
69	446.0
70	364.0
71	282.0
72	245.0
73	208.0
74	154.0
75	80.0
76	60.0
77	55.0
78	50.0
79	36.0
80	22.0
81	17.0
82	12.0
83	8.5
84	5.0
85	2.5
86	0.0
87	0.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	261551.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.4246754545011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.03532721688491	40.861800752607216
2	7.601121417950877	7.665681611808603
3	3.100360448143071	4.690040081687577
4	1.7549905248165982	3.539793105584061
5	1.037787157351903	2.616504030015949
6	0.8126403825950047	2.458627653214483
7	0.6031663535665421	2.1290127336567424
8	0.4611838715365653	1.8604037637665307
9	0.39776509440860447	1.8051458213414584
>10	3.142824310342533	26.96715483036929
>50	0.039247536642512106	1.3902786190550873
>100	0.009811884160628026	1.2380444423176018
>500	0.0022642809601449293	0.7227317540612129
>1k	0.0015095206400966195	2.054780800514212
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3900	1.4911049852610008	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1499	0.5731195827964718	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	714	0.27298691268624475	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	667	0.2550171859407917	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	518	0.19804932881158935	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	455	0.1739622482804501	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	426	0.1628745445438939	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	394	0.15063983697252162	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	389	0.14872816391449467	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	367	0.14031680245917622	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	298	0.11393571425840467	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.823346116053848E-4	0.0	0.0	0.1104947027539562	0.0
2	3.823346116053848E-4	0.0	0.0	0.5039170180958972	0.0
3	3.823346116053848E-4	0.0	0.0	0.6553215242916296	0.0
4	3.823346116053848E-4	0.0	0.0	1.0223627514327989	0.0
5	3.823346116053848E-4	0.0	0.0	2.268391250654748	0.0
6	3.823346116053848E-4	0.0	0.0	2.766190914964959	0.0
7	3.823346116053848E-4	0.0	0.0	3.2720196061188833	0.0
8	3.823346116053848E-4	0.0	0.0	4.161712247324614	0.0
9	3.823346116053848E-4	0.0	0.0	4.479814644180293	0.0
10	3.823346116053848E-4	0.0	0.0	5.835190842321383	0.0
11	3.823346116053848E-4	0.0	0.0	6.685120683920153	0.0
12	3.823346116053848E-4	0.0	0.0	8.101670419918104	0.0
13	3.823346116053848E-4	0.0	0.0	8.493181062202018	0.0
14	3.823346116053848E-4	0.0	0.0	8.671731325821733	0.0
15	3.823346116053848E-4	0.0	0.0	9.025008506945108	0.0
16	3.823346116053848E-4	0.0	0.0	9.556453617076594	0.0
17	3.823346116053848E-4	0.0	0.0	10.066105654346572	0.0
18	3.823346116053848E-4	0.0	0.0	10.73710289771402	0.0
19	3.823346116053848E-4	0.0	0.0	11.107967470971245	0.0
20	3.823346116053848E-4	0.0	0.0	11.503683793982818	0.0
21	3.823346116053848E-4	0.0	0.0	11.977778712373494	0.0
22	3.823346116053848E-4	0.0	0.0	12.501959464884477	0.0
23	3.823346116053848E-4	0.0	0.0	13.018493525163352	0.0
24	3.823346116053848E-4	0.0	0.0	13.447472959384594	0.0
25	3.823346116053848E-4	0.0	0.0	13.792721113664257	0.0
26	3.823346116053848E-4	0.0	0.0	14.120764210421678	0.0
27	3.823346116053848E-4	0.0	0.0	14.474423726156658	0.0
28	3.823346116053848E-4	0.0	0.0	14.846817637860303	0.0
29	3.823346116053848E-4	0.0	0.0	15.221505557233579	0.0
30	3.823346116053848E-4	0.0	0.0	15.691394794896597	0.0
31	3.823346116053848E-4	0.0	0.0	16.110051194604495	0.0
32	3.823346116053848E-4	0.0	0.0	16.54438331338821	0.0
33	3.823346116053848E-4	0.0	0.0	16.970304070716608	0.0
34	3.823346116053848E-4	0.0	0.0	17.388195801201295	0.0
35	3.823346116053848E-4	0.0	0.0	17.846997335127757	0.0
36	3.823346116053848E-4	0.0	0.0	18.31803357662559	0.0
37	3.823346116053848E-4	0.0	0.0	18.81277456404296	0.0
38	3.823346116053848E-4	0.0	0.0	19.260106059621258	0.0
39	3.823346116053848E-4	0.0	0.0	19.715466582043273	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCTCA	30	2.1590513E-6	45.000004	7
AATGGGC	60	0.0	45.000004	4
CACTGGG	25	3.8825172E-5	45.000004	3
TCAATCG	25	3.8825172E-5	45.000004	30
TAGGGCG	60	0.0	45.000004	5
CAATCGC	25	3.8825172E-5	45.000004	31
ATCGCTA	25	3.8825172E-5	45.000004	33
ATACATG	25	3.8825172E-5	45.000004	42
AATCGCT	30	2.1590513E-6	45.000004	32
ATGGGCG	45	3.8198777E-10	45.0	5
ATCTAAT	20	7.023287E-4	45.0	22
CGGTTTA	140	0.0	45.0	1
TCTTAGG	45	3.8198777E-10	45.0	2
ATAGCGG	35	1.2071177E-7	45.0	2
TAAACGG	20	7.023287E-4	45.0	2
TACGGTT	35	1.2071177E-7	45.0	33
CCTCGTG	55	1.8189894E-12	45.0	15
GGGACGT	20	7.023287E-4	45.0	7
CTACGGG	55	1.8189894E-12	45.0	3
ACTACGG	20	7.023287E-4	45.0	2
>>END_MODULE