SRR3549459_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549459_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	234557
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base quality graph

Per Sequence quality graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4222	1.7999889152743258	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1688	0.7196544976274424	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCG	1408	0.6002805288266817	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTC	1083	0.4617214578972275	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGC	974	0.41525087718550285	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	509	0.21700482185566836	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	420	0.17906095320114088	No Hit
GCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC	350	0.14921746100095074	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCT	324	0.1381327353265944	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTC	312	0.1330167080922761	No Hit
GAACTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCT	264	0.11255259915500283	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	254	0.10828924312640426	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	253	0.10786290752354437	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	35	1.2064083E-7	45.000004	5
GGATCCC	35	1.2064083E-7	45.000004	8
TGCGGGC	30	2.1580909E-6	45.000004	4
CGTTAGG	35	1.2064083E-7	45.000004	2
CGGGGTA	35	1.2064083E-7	45.000004	6
TTGGGCG	30	2.1580909E-6	45.000004	5
CGGGTCA	25	3.8812836E-5	45.0	6
TCGTTGA	20	7.0217956E-4	45.0	24
GGAGCAC	20	7.0217956E-4	45.0	8
CGCGGGC	20	7.0217956E-4	45.0	4
CGCGGCC	20	7.0217956E-4	45.0	35
ACGTTAG	20	7.0217956E-4	45.0	1
CGGTTTA	100	0.0	45.0	1
TTTCGAC	20	7.0217956E-4	45.0	24
CGTTGAT	20	7.0217956E-4	45.0	25
GGGTACC	20	7.0217956E-4	45.0	7
CGATTAT	20	7.0217956E-4	45.0	16
CGACAGG	40	6.7757355E-9	45.0	2
TACGGCA	20	7.0217956E-4	45.0	7
ACGGGTC	20	7.0217956E-4	45.0	5