FastQCFastQC Report
Sat 18 Jun 2016
SRR3549455_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549455_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences181876
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31361.7242516879632277No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12360.6795838923222416No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCG10440.5740174624469419No Hit
GAATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC8310.4569047043040313No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGC7560.4156678176339924No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5090.27986100420066423No Hit
GCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC4340.23862411753062523TruSeq Adapter, Index 13 (95% over 23bp)
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4050.2226791880182102No Hit
CTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCT3550.19518793023818426TruSeq Adapter, Index 16 (95% over 22bp)
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.1880402032153775No Hit
CCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC2800.15395104356814535TruSeq Adapter, Index 13 (95% over 23bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA2770.15230156810134376No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCT2760.15175174294574326No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTC2710.14900261716774066No Hit
GAACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT2270.12481031032131783No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC2030.11161450658690537No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2010.11051485627570433No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2010.11051485627570433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGA302.1553988E-645.0000044
CGGTTTA1050.045.0000041
GCACGGG302.1553988E-645.0000043
GGTATGC302.1553988E-645.0000048
TCACGAC207.0176146E-445.025
ATCCTGC207.0176146E-445.020
CTCACGT207.0176146E-445.045
TGCCAGT207.0176146E-445.024
ATGCTGG351.2044075E-745.02
ACGGGAA800.045.05
ATCAACC207.0176146E-445.010
CTACGGG453.8016879E-1045.03
CGAATGG351.2044075E-745.02
AGCTCAC207.0176146E-445.043
GCTACGG207.0176146E-445.02
CTGGGCG406.7611836E-945.05
TGGGACC207.0176146E-445.06
GTTGGGC351.2044075E-745.04
GTACGGG351.2044075E-745.03
GCTCACG207.0176146E-445.044
Produced by FastQC (version 0.11.5)