SRR3549453_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549453_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	120486
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2480	2.058330428431519	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	970	0.8050727885397473	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	885	0.7345251730491509	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	592	0.4913433925933303	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	576	0.4780638414421593	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	408	0.33862855435486283	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	351	0.2913201533788158	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	288	0.23903192072107965	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	281	0.23322211709244228	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	277	0.2299022293046495	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	231	0.19172351974503263	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	230	0.19089354779808443	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT	227	0.18840363195723986	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	188	0.1560347260262603	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTC	187	0.1552047540793121	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	155	0.12864565177696993	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	130	0.1078963531032651	No Hit
GTGTCCGGGATAGTTGATTTTGTGGACTGAATTTAATGCTTCCAAATGTTT	127	0.10540643726242054	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTT	123	0.10208654947462775	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAATG	25	3.8699727E-5	45.000004	45
GGAATCG	20	7.008135E-4	45.000004	7
CGGAAGG	25	3.8699727E-5	45.000004	2
TCTTGAC	20	7.008135E-4	45.000004	13
CGAACAA	20	7.008135E-4	45.000004	12
CATATAC	20	7.008135E-4	45.000004	12
CCCTAGC	25	3.8699727E-5	45.000004	21
TATGGCA	20	7.008135E-4	45.000004	36
GAATCGA	20	7.008135E-4	45.000004	8
GTAGGTT	20	7.008135E-4	45.000004	45
AATGGGC	20	7.008135E-4	45.000004	4
ACGGGTA	20	7.008135E-4	45.000004	5
GCGCAGG	25	3.8699727E-5	45.000004	2
CCCACCA	20	7.008135E-4	45.000004	14
GACCATT	20	7.008135E-4	45.000004	9
TAGTTCC	20	7.008135E-4	45.000004	44
TACGCGG	20	7.008135E-4	45.000004	2
GGGACGA	20	7.008135E-4	45.000004	7
CTACGCG	20	7.008135E-4	45.000004	1
GCCCTAG	25	3.8699727E-5	45.000004	20

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content