##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549453_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	120486
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.536601762860414	31.0	31.0	34.0	30.0	34.0
2	31.544270703650216	31.0	31.0	34.0	30.0	34.0
3	30.627865478146838	31.0	31.0	34.0	26.0	34.0
4	35.14268877711933	37.0	35.0	37.0	32.0	37.0
5	35.224225221187524	37.0	35.0	37.0	33.0	37.0
6	35.32151453280879	37.0	35.0	37.0	33.0	37.0
7	32.0618163106087	37.0	35.0	37.0	10.0	37.0
8	33.667148050395895	37.0	35.0	37.0	19.0	37.0
9	36.73245024318178	39.0	35.0	39.0	32.0	39.0
10	36.73842604119981	38.0	35.0	39.0	32.0	39.0
11	36.660790465282275	39.0	35.0	39.0	32.0	39.0
12	36.411881878392514	38.0	35.0	39.0	32.0	39.0
13	32.54134920239696	37.0	33.0	39.0	10.0	39.0
14	35.103439403748155	38.0	34.0	40.0	18.0	41.0
15	36.53675115781087	39.0	35.0	40.0	30.0	41.0
16	37.22131201965374	39.0	35.0	41.0	32.0	41.0
17	37.33071062198098	39.0	35.0	40.0	32.0	41.0
18	37.18673538834388	39.0	36.0	40.0	32.0	41.0
19	37.056330196039376	38.0	35.0	40.0	32.0	41.0
20	36.871752734757564	38.0	35.0	40.0	32.0	41.0
21	36.836512125890145	38.0	35.0	40.0	32.0	41.0
22	36.80253307438208	38.0	35.0	40.0	32.0	41.0
23	36.735064654814664	38.0	35.0	40.0	32.0	41.0
24	36.57571004100061	38.0	35.0	40.0	32.0	41.0
25	36.43188420231396	38.0	35.0	40.0	32.0	41.0
26	36.25619574058397	38.0	35.0	40.0	31.0	41.0
27	36.22697242833192	38.0	35.0	40.0	31.0	41.0
28	36.14535298706904	37.0	35.0	40.0	31.0	41.0
29	36.16389456036386	38.0	35.0	40.0	31.0	41.0
30	35.99347642049699	37.0	35.0	40.0	31.0	41.0
31	35.788996231927364	37.0	35.0	40.0	30.0	41.0
32	35.458020018923364	37.0	34.0	40.0	29.0	41.0
33	35.06878807496307	37.0	34.0	40.0	27.0	41.0
34	34.71589230284017	37.0	34.0	40.0	24.0	41.0
35	34.370814866457515	37.0	34.0	40.0	22.0	41.0
36	34.08132895108145	37.0	33.0	40.0	20.0	41.0
37	33.914396693391765	37.0	33.0	40.0	18.0	41.0
38	33.733330013445546	37.0	33.0	40.0	18.0	41.0
39	33.62591504407151	37.0	33.0	40.0	18.0	41.0
40	33.447495974636055	37.0	33.0	40.0	17.0	41.0
41	33.46252676659529	37.0	33.0	40.0	16.0	41.0
42	33.4215676510134	37.0	33.0	40.0	15.0	41.0
43	33.37722224988796	37.0	33.0	40.0	15.0	41.0
44	33.14032335707053	36.0	33.0	40.0	15.0	41.0
45	33.09260826984048	36.0	33.0	40.0	15.0	41.0
46	33.01470710289992	36.0	33.0	40.0	15.0	41.0
47	32.82611257739488	36.0	32.0	40.0	15.0	41.0
48	32.753896718290925	36.0	32.0	40.0	13.0	41.0
49	32.70730209318925	36.0	32.0	40.0	13.0	41.0
50	32.53506631475856	35.0	31.0	40.0	12.0	41.0
51	30.761258569460352	35.0	28.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	5.0
11	3.0
12	1.0
13	4.0
14	1.0
15	11.0
16	17.0
17	31.0
18	82.0
19	164.0
20	281.0
21	454.0
22	729.0
23	1099.0
24	1728.0
25	2411.0
26	3001.0
27	3066.0
28	2830.0
29	2803.0
30	3282.0
31	3912.0
32	5241.0
33	7580.0
34	10874.0
35	13011.0
36	10807.0
37	14736.0
38	17404.0
39	14917.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.302624371296254	21.214082963995818	24.02021811662766	21.463074548080275
2	35.0704646182959	27.884567501618445	25.151469880318047	11.893497999767607
3	28.897133276895243	27.10522384343409	32.85194960410338	11.145693275567286
4	23.225934963398238	25.086732068456087	38.89746526567402	12.789867702471657
5	23.032551499759307	29.75117441030493	34.37245821091247	12.84381587902329
6	19.53089985558488	40.62546685922016	31.646000365187653	8.197632920007305
7	65.06150092126887	15.473996978902113	17.286655711036968	2.1778463887920587
8	71.082947396378	8.97614660624471	16.921468054379762	3.0194379429975267
9	66.05165745397805	9.077403183772388	18.881861793071394	5.9890775691781615
10	37.42758494762877	27.21644008432515	24.931527314376776	10.424447653669306
11	33.354912603953984	22.682303338147168	31.748916886609237	12.21386717128961
12	30.179439934930198	22.241588234317682	34.10935710372989	13.469614727022227
13	16.97292631509055	38.826917650183425	32.36558604319174	11.834569991534286
14	14.041465398469533	36.04402171206613	35.486280563716946	14.42823232574739
15	13.891240476071909	25.717510748136714	48.35499560114868	12.036253174642697
16	14.833258635858106	24.008598509370383	45.41855485284597	15.739588001925533
17	15.193466460833625	23.516425144830105	36.79597629600119	24.494132098335076
18	17.010275052703218	26.56325216207692	40.39805454575635	16.02841823946351
19	21.915409259167042	25.8619258669057	35.1202629351128	17.102401938814467
20	23.42595820261275	25.402121408296402	36.3104427070365	14.861477682054346
21	18.349849775077605	28.091230516408544	36.87565360290822	16.68326610560563
22	18.25440300117856	26.088508208422557	34.820643062264494	20.83644572813439
23	16.428464717892535	28.733628803346445	35.068804674402	19.769101804359014
24	17.494978669720965	25.909234267881747	40.814700463124346	15.781086599272944
25	15.556994173596934	29.273940540809722	37.932207891373274	17.236857394220078
26	15.028302043390934	31.73148747572332	35.97679398436333	17.263416496522417
27	17.131450956957657	31.533124180402705	35.49209036734558	15.843334495294059
28	13.157545274969706	29.950367677572498	41.486147768205434	15.405939279252362
29	15.443288016865031	26.069418853642745	40.17977192370898	18.307521205783246
30	16.895738924024368	30.562057002473313	37.66080706472121	14.881397008781102
31	22.08970336802616	27.92025629533722	34.217253456833156	15.772786879803464
32	22.671513702836844	28.72366913998307	35.06963464634895	13.535182510831135
33	20.75510847733347	30.223428448118455	32.0377471241472	16.983715950400878
34	18.46106601596866	29.356107763557592	34.965058181033484	17.21776803944027
35	19.743372674003616	27.532659396112415	33.68026160715768	19.04370632272629
36	22.547017910794615	28.624072506349286	33.85538568796375	14.973523894892352
37	19.562438789568912	30.773699848945107	35.032285908736284	14.631575452749699
38	17.948973324701626	32.0377471241472	30.244177746792154	19.769101804359014
39	21.21657287983666	28.597513404046943	33.12750029049018	17.058413425626213
40	19.727603207011605	28.48878707899673	33.19555799013993	18.588051723851734
41	16.987035838188667	27.400693856547647	33.563235562637985	22.0490347426257
42	18.68681838553857	28.00823332171373	33.26610560563053	20.038842687117175
43	20.823996148930167	26.056139302491577	32.016167853526554	21.103696695051706
44	18.280132131533954	29.94538784589081	32.02114768520824	19.753332337366995
45	16.736384310210315	33.21298740102584	30.517238517338114	19.53338977142573
46	21.302059990372328	31.3745995385356	30.667463439735737	16.65587703135634
47	18.675198778281292	28.641501917235196	34.067028534435536	18.616270770047972
48	19.87699815746228	28.173397739156414	32.55813953488372	19.391464568497586
49	18.749066281559685	26.169015487276532	35.00821672227479	20.073701508889
50	17.963912819746692	29.582690105074448	32.729943727902	19.723453347276863
51	17.309894925551518	29.04901814318676	30.244177746792154	23.396909184469564
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	328.0
1	408.5
2	489.0
3	2285.5
4	4082.0
5	3475.0
6	2868.0
7	2220.5
8	1573.0
9	1528.5
10	1484.0
11	1423.0
12	1362.0
13	1309.5
14	1257.0
15	1230.0
16	1203.0
17	1163.5
18	1124.0
19	1103.5
20	1083.0
21	1031.0
22	979.0
23	1123.5
24	1268.0
25	1519.0
26	1867.0
27	1964.0
28	2421.5
29	2879.0
30	3107.0
31	3335.0
32	3463.0
33	3591.0
34	3773.5
35	3956.0
36	4259.5
37	4563.0
38	5098.0
39	5633.0
40	6723.0
41	7813.0
42	8867.5
43	9922.0
44	10419.0
45	10916.0
46	12170.0
47	13424.0
48	11348.0
49	9272.0
50	8127.0
51	6982.0
52	5965.0
53	4948.0
54	4129.0
55	3310.0
56	2857.0
57	2404.0
58	2037.0
59	1670.0
60	1399.5
61	1129.0
62	961.5
63	794.0
64	615.0
65	436.0
66	341.0
67	246.0
68	202.0
69	158.0
70	130.5
71	103.0
72	88.5
73	74.0
74	62.5
75	34.5
76	18.0
77	16.0
78	14.0
79	10.5
80	7.0
81	4.0
82	1.0
83	1.0
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	120486.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.001078963531036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.57838436001475	34.91111000448185
2	8.613057912209516	7.751937984496124
3	3.587237181851715	4.842886310442707
4	2.104389524160826	3.7879919658715537
5	1.2984138694208778	2.92150125325764
6	0.9774990778310587	2.6393107912952543
7	0.783843600147547	2.4691665421708744
8	0.6086315012910365	2.19112593994323
9	0.5459240132792327	2.2110452666699865
>10	3.817779417189229	27.378284613980046
>50	0.03873109553670233	1.1055226333349932
>100	0.03688675765400221	3.222781069999834
>500	0.0073773515308004425	2.509005195624388
>1k	0.0018443378827001106	2.058330428431519
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2480	2.058330428431519	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	970	0.8050727885397473	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	885	0.7345251730491509	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	592	0.4913433925933303	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	576	0.4780638414421593	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	408	0.33862855435486283	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	351	0.2913201533788158	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	288	0.23903192072107965	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	281	0.23322211709244228	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	277	0.2299022293046495	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	231	0.19172351974503263	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	230	0.19089354779808443	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT	227	0.18840363195723986	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	188	0.1560347260262603	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTC	187	0.1552047540793121	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	155	0.12864565177696993	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	130	0.1078963531032651	No Hit
GTGTCCGGGATAGTTGATTTTGTGGACTGAATTTAATGCTTCCAAATGTTT	127	0.10540643726242054	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTT	123	0.10208654947462775	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1543747821323639	0.0
2	0.0	0.0	0.0	0.5793204189698388	0.0
3	0.0	0.0	0.0	0.6930265757017413	0.0
4	0.0	0.0	0.0	1.009245887489003	0.0
5	0.0	0.0	0.0	2.015171887190213	0.0
6	0.0	0.0	0.0	2.3529704695981275	0.0
7	0.0	0.0	0.0	2.7563368358149494	0.0
8	0.0	0.0	0.0	3.4601530468270174	0.0
9	0.0	0.0	0.0	3.636937071526982	0.0
10	0.0	0.0	0.0	4.88272496389622	0.0
11	0.0	0.0	0.0	5.393157711269359	0.0
12	0.0	0.0	0.0	6.391613963448036	0.0
13	0.0	0.0	0.0	6.61902627691184	0.0
14	0.0	0.0	0.0	6.721112826386468	0.0
15	0.0	0.0	0.0	6.930265757017413	0.0
16	0.0	0.0	0.0	7.2207559384492805	0.0
17	0.0	0.0	0.0	7.5079262320933555	0.0
18	0.0	0.0	0.0	7.875603804591405	0.0
19	0.0	0.0	0.0	8.10301611805521	0.0
20	0.0	0.0	0.0	8.3370682070946	0.0
21	0.0	0.0	0.0	8.653287518881863	0.0
22	0.0	0.0	0.0	8.92966817721561	0.0
23	0.0	0.0	0.0	9.25584715236625	0.0
24	0.0	0.0	0.0	9.512308483973241	0.0
25	0.0	0.0	0.0	9.709011835399963	0.0
26	0.0	0.0	0.0	9.928124429394286	0.0
27	0.0	0.0	0.0	10.131467556396593	0.0
28	0.0	0.0	0.0	10.359709841807346	0.0
29	0.0	0.0	0.0	10.63360058430025	0.0
30	0.0	0.0	0.0	10.949819896087511	0.0
31	0.0	0.0	0.0	11.269359095662567	0.0
32	0.0	0.0	0.0	11.565659080723071	0.0
33	0.0	0.0	0.0	11.839549823215975	0.0
34	8.299719469481931E-4	0.0	0.0	12.134189864382584	0.0
35	8.299719469481931E-4	0.0	0.0	12.47115847484355	0.0
36	8.299719469481931E-4	0.0	0.0	12.840495991235496	0.0
37	8.299719469481931E-4	0.0	0.0	13.170824826120876	0.0
38	8.299719469481931E-4	0.0	0.0	13.493683913483725	0.0
39	8.299719469481931E-4	0.0	0.0	13.830652523944691	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAATG	25	3.8699727E-5	45.000004	45
GGAATCG	20	7.008135E-4	45.000004	7
CGGAAGG	25	3.8699727E-5	45.000004	2
TCTTGAC	20	7.008135E-4	45.000004	13
CGAACAA	20	7.008135E-4	45.000004	12
CATATAC	20	7.008135E-4	45.000004	12
CCCTAGC	25	3.8699727E-5	45.000004	21
TATGGCA	20	7.008135E-4	45.000004	36
GAATCGA	20	7.008135E-4	45.000004	8
GTAGGTT	20	7.008135E-4	45.000004	45
AATGGGC	20	7.008135E-4	45.000004	4
ACGGGTA	20	7.008135E-4	45.000004	5
GCGCAGG	25	3.8699727E-5	45.000004	2
CCCACCA	20	7.008135E-4	45.000004	14
GACCATT	20	7.008135E-4	45.000004	9
TAGTTCC	20	7.008135E-4	45.000004	44
TACGCGG	20	7.008135E-4	45.000004	2
GGGACGA	20	7.008135E-4	45.000004	7
CTACGCG	20	7.008135E-4	45.000004	1
GCCCTAG	25	3.8699727E-5	45.000004	20
>>END_MODULE