FastQCFastQC Report
Sat 18 Jun 2016
SRR3549452_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549452_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60928
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9961.634716386554622No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3880.6368172268907563No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG2680.43986344537815125No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC2490.4086790966386555No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTC2370.388983718487395No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1420.23306197478991597No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1350.22157300420168066No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA1300.21336659663865545No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.1871060924369748No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC1100.18054096638655462No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1020.16741071428571427No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT970.15920430672268907No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG830.13622636554621848No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA820.13458508403361344No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT680.11160714285714285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTAA453.7289283E-1045.0000048
TTGCGGG302.1317046E-645.0000043
CGGTTTA453.7289283E-1045.0000041
TAAGGGC302.1317046E-645.0000044
GATGGCC206.9807336E-445.09
GAGCGGG253.8473132E-545.03
TGCACCA253.8473132E-545.028
TATGCCC253.8473132E-545.010
TACGGGA206.9807336E-445.04
CTAAGGG206.9807336E-445.03
TCGTGTA253.8473132E-545.017
AGGGACC253.8473132E-545.06
CACCTTC253.8473132E-545.033
AGAGCGG253.8473132E-545.02
CGGGATT206.9807336E-445.06
ATGCCCT253.8473132E-545.011
GGGACTA406.6338544E-945.07
CCATAGC206.9807336E-445.035
GGGAATC253.8473132E-545.07
GTGCGGG206.9807336E-445.03