##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549452_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	60928
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.607027967436974	31.0	31.0	34.0	30.0	34.0
2	31.688977153361346	31.0	31.0	34.0	30.0	34.0
3	31.000935530462186	31.0	31.0	34.0	27.0	34.0
4	35.316094406512605	37.0	35.0	37.0	33.0	37.0
5	35.29185267857143	37.0	35.0	37.0	33.0	37.0
6	35.35148043592437	37.0	35.0	37.0	33.0	37.0
7	32.07551536239496	37.0	35.0	37.0	10.0	37.0
8	33.643546481092436	37.0	35.0	37.0	18.0	37.0
9	36.70076155462185	39.0	35.0	39.0	32.0	39.0
10	36.67445181197479	38.0	35.0	39.0	32.0	39.0
11	36.675912552521005	39.0	35.0	39.0	32.0	39.0
12	36.6039587710084	38.0	35.0	39.0	32.0	39.0
13	32.88950892857143	38.0	35.0	39.0	10.0	39.0
14	35.46226693802521	39.0	35.0	40.0	18.0	41.0
15	36.80795365021008	39.0	35.0	41.0	30.0	41.0
16	37.435005252100844	39.0	36.0	41.0	32.0	41.0
17	37.53415506827731	39.0	36.0	41.0	33.0	41.0
18	37.43557970063025	39.0	36.0	40.0	33.0	41.0
19	37.39461331407563	39.0	36.0	40.0	32.0	41.0
20	37.25756630777311	39.0	35.0	40.0	32.0	41.0
21	37.20488117121849	39.0	35.0	40.0	32.0	41.0
22	37.199103860294116	39.0	35.0	40.0	33.0	41.0
23	37.11255908613445	39.0	35.0	40.0	32.0	41.0
24	36.98852744222689	39.0	35.0	40.0	32.0	41.0
25	36.80621389180672	38.0	35.0	40.0	32.0	41.0
26	36.66041885504202	38.0	35.0	40.0	31.0	41.0
27	36.68443080357143	38.0	35.0	40.0	32.0	41.0
28	36.57922465861345	38.0	35.0	40.0	31.0	41.0
29	36.510783219537814	38.0	35.0	40.0	31.0	41.0
30	36.357077205882355	38.0	35.0	40.0	31.0	41.0
31	36.198890493697476	38.0	35.0	40.0	30.0	41.0
32	35.89369419642857	38.0	35.0	40.0	30.0	41.0
33	35.63105632878151	38.0	35.0	40.0	28.0	41.0
34	35.33482142857143	38.0	34.0	40.0	26.0	41.0
35	35.124671743697476	38.0	34.0	40.0	25.0	41.0
36	34.89556525735294	38.0	34.0	40.0	24.0	41.0
37	34.795069590336134	38.0	34.0	40.0	23.0	41.0
38	34.67814469537815	38.0	34.0	40.0	23.0	41.0
39	34.51797203256302	38.0	34.0	40.0	22.0	41.0
40	34.39523700105042	38.0	33.0	40.0	22.0	41.0
41	34.402245273109244	38.0	34.0	40.0	21.0	41.0
42	34.36004792542017	38.0	33.0	40.0	21.0	41.0
43	34.32078847163866	38.0	33.0	40.0	21.0	41.0
44	34.12549238445378	38.0	33.0	40.0	20.0	41.0
45	34.138540572478995	38.0	33.0	40.0	20.0	41.0
46	33.97546284138655	37.0	33.0	40.0	20.0	41.0
47	33.764410451680675	37.0	33.0	40.0	18.0	41.0
48	33.683659401260506	37.0	33.0	40.0	18.0	41.0
49	33.61173844537815	37.0	33.0	40.0	18.0	41.0
50	33.48156840861345	37.0	33.0	40.0	18.0	41.0
51	31.665523240546218	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	3.0
12	0.0
13	2.0
14	1.0
15	2.0
16	3.0
17	15.0
18	39.0
19	63.0
20	93.0
21	175.0
22	268.0
23	425.0
24	634.0
25	1005.0
26	1184.0
27	1290.0
28	1336.0
29	1297.0
30	1579.0
31	1887.0
32	2443.0
33	3363.0
34	4857.0
35	5855.0
36	5916.0
37	8614.0
38	9998.0
39	8578.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.6577599789916	22.267266281512605	25.52521008403361	18.549763655462183
2	34.47019432773109	27.85090598739496	25.54654674369748	12.132352941176471
3	30.252100840336134	26.02908350840336	31.97544642857143	11.743369222689076
4	26.40329569327731	24.983587184873947	35.34827993697479	13.264837184873949
5	23.16340598739496	30.865940126050422	31.54871323529412	14.421940651260504
6	20.763852415966387	41.36193539915966	29.53486081932773	8.339351365546218
7	68.88950892857143	15.134256827731093	13.831079306722687	2.14515493697479
8	74.81289390756302	8.224461659663866	14.159335609243698	2.8033088235294117
9	68.16077993697479	9.324120273109243	16.173188025210084	6.341911764705882
10	34.338891806722685	30.47203256302521	23.511357668067227	11.677717962184873
11	29.643185399159666	25.154280462184875	30.852809873949578	14.349724264705882
12	28.63215598739496	22.533153886554622	33.62001050420168	15.21467962184874
13	19.537815126050422	34.586725315126046	30.757615546218485	15.117844012605042
14	16.032037815126053	32.77803308823529	33.56748949579832	17.622439600840337
15	17.297465861344538	27.371651785714285	40.61351102941176	14.717371323529413
16	18.249409138655462	26.053702731092436	38.309151785714285	17.387736344537817
17	18.052455357142858	26.603532037815125	34.45706407563025	20.886948529411764
18	18.538274684873947	28.673188025210084	35.658482142857146	17.130055147058822
19	22.889311974789916	27.676930147058826	32.671349789915965	16.762408088235293
20	23.233981092436977	26.061909138655466	35.294117647058826	15.40999212184874
21	20.647321428571427	28.535320378151262	34.87066701680672	15.946691176470587
22	19.268644957983195	25.787815126050422	34.204306722689076	20.73923319327731
23	18.90920430672269	29.08514968487395	34.32576155462185	17.679884453781515
24	18.54155724789916	26.779149159663866	36.723673844537814	17.95561974789916
25	17.885044642857142	30.89876575630252	33.7250525210084	17.491137079831933
26	16.503085609243698	31.35340073529412	33.05540966386555	19.08810399159664
27	18.735228466386555	30.388327205882355	33.475577731092436	17.400866596638657
28	15.759585084033615	30.099461659663866	37.57057510504202	16.570378151260503
29	18.123030462184875	27.798384978991596	35.10372899159664	18.974855567226893
30	17.935924369747898	30.2734375	34.27488182773109	17.51575630252101
31	22.039128151260503	29.14423581932773	31.635701155462186	17.180934873949578
32	21.323529411764707	29.981289390756306	32.38740808823529	16.307773109243698
33	19.761029411764707	30.88891806722689	30.55902048319328	18.791032037815125
34	18.845194327731093	30.238970588235293	32.592568277310924	18.32326680672269
35	19.808626575630253	28.3859637605042	32.02796743697479	19.777442226890756
36	20.92469800420168	31.860556722689076	29.305081407563026	17.909663865546218
37	18.965007878151262	31.335346638655466	32.84696691176471	16.852678571428573
38	18.23956144957983	32.18224789915966	30.24717699579832	19.331013655462183
39	20.392922794117645	29.37565651260504	31.65703781512605	18.574382878151262
40	19.608390231092436	29.920561974789916	32.64016544117647	17.830882352941178
41	17.72091649159664	29.936974789915965	31.73253676470588	20.609571953781515
42	18.677783613445378	30.21763392857143	30.977547268907564	20.12703518907563
43	20.43231355042017	27.69170168067227	30.929950105042014	20.946034663865547
44	19.015887605042018	29.44951418067227	31.287749474789916	20.2468487394958
45	18.54483981092437	31.284466911764707	29.41832983193277	20.75236344537815
46	19.337578781512605	32.810858718487395	29.093356092436974	18.758206407563023
47	18.682707457983195	28.582917542016805	33.370535714285715	19.363839285714285
48	18.690913865546218	28.507418592436974	32.52691701680672	20.274750525210084
49	18.746717436974787	27.64410451680672	34.14522058823529	19.463957457983195
50	17.08409926470588	29.155724789915965	33.48542542016807	20.274750525210084
51	17.584690126050422	27.461922268907564	31.586462710084035	23.366924894957982
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	71.0
1	109.5
2	148.0
3	902.5
4	1657.0
5	1429.0
6	1201.0
7	900.0
8	599.0
9	571.5
10	544.0
11	521.5
12	499.0
13	498.5
14	498.0
15	448.0
16	398.0
17	390.0
18	382.0
19	362.5
20	343.0
21	390.5
22	438.0
23	479.5
24	521.0
25	671.0
26	947.0
27	1073.0
28	1271.0
29	1469.0
30	1636.5
31	1804.0
32	1982.0
33	2160.0
34	2355.5
35	2551.0
36	2517.0
37	2483.0
38	2741.5
39	3000.0
40	3540.5
41	4081.0
42	4395.0
43	4709.0
44	4858.0
45	5007.0
46	5537.5
47	6068.0
48	5488.5
49	4909.0
50	4475.5
51	4042.0
52	3532.0
53	3022.0
54	2498.0
55	1974.0
56	1738.5
57	1503.0
58	1307.0
59	1111.0
60	925.5
61	740.0
62	603.5
63	467.0
64	366.5
65	266.0
66	203.5
67	141.0
68	111.5
69	82.0
70	70.0
71	58.0
72	45.5
73	33.0
74	27.0
75	19.5
76	18.0
77	12.5
78	7.0
79	6.5
80	6.0
81	3.0
82	0.0
83	1.0
84	2.0
85	1.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	60928.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.66366859243697
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.98373250148238	50.28394170168067
2	10.183299389002038	12.966123949579833
3	4.668849416071566	8.917082457983193
4	2.6553919925752147	6.762079831932772
5	1.5030034288071359	4.784335609243697
6	0.7940395472943361	3.0330882352941178
7	0.4485807832117353	1.9990808823529411
8	0.2706953002139782	1.3786764705882353
9	0.10827812008559128	0.6204044117647058
>10	0.3428807136043724	3.917738970588235
>50	0.012890252391141818	0.6253282563025211
>100	0.025780504782283636	3.077402836134454
>500	0.0025780504782283637	1.634716386554622
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	996	1.634716386554622	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	388	0.6368172268907563	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG	268	0.43986344537815125	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC	249	0.4086790966386555	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTC	237	0.388983718487395	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	142	0.23306197478991597	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	135	0.22157300420168066	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	130	0.21336659663865545	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	114	0.1871060924369748	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	110	0.18054096638655462	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	102	0.16741071428571427	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	97	0.15920430672268907	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	83	0.13622636554621848	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	82	0.13458508403361344	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	68	0.11160714285714285	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09847689075630252	0.0
2	0.0	0.0	0.0	0.38570115546218486	0.0
3	0.0	0.0	0.0	0.48417804621848737	0.0
4	0.0	0.0	0.0	0.7352941176470589	0.0
5	0.0	0.0	0.0	1.6265099789915967	0.0
6	0.0	0.0	0.0	1.9055278361344539	0.0
7	0.0	0.0	0.0	2.1779805672268906	0.0
8	0.0	0.0	0.0	2.7179621848739495	0.0
9	0.0	0.0	0.0	2.895220588235294	0.0
10	0.0	0.0	0.0	3.824185924369748	0.0
11	0.0	0.0	0.0	4.351037289915967	0.0
12	0.0	0.0	0.0	5.345653886554622	0.0
13	0.0	0.0	0.0	5.604976365546219	0.0
14	0.0	0.0	0.0	5.726431197478991	0.0
15	0.0	0.0	0.0	5.948004201680672	0.0
16	0.0	0.0	0.0	6.333705357142857	0.0
17	0.0	0.0	0.0	6.701352415966387	0.0
18	0.0	0.0	0.0	7.160911239495798	0.0
19	0.0	0.0	0.0	7.453059348739496	0.0
20	0.0	0.0	0.0	7.743566176470588	0.0
21	0.0	0.0	0.0	8.086594012605042	0.0
22	0.0	0.0	0.0	8.531381302521009	0.0
23	0.0	0.0	0.0	8.992581407563025	0.0
24	0.0	0.0	0.0	9.317555147058824	0.0
25	0.0	0.0	0.0	9.611344537815127	0.0
26	0.0	0.0	0.0	9.913340336134453	0.0
27	0.0	0.0	0.0	10.239955357142858	0.0
28	0.0	0.0	0.0	10.584624474789916	0.0
29	0.0	0.0	0.0	10.921087184873949	0.0
30	0.0	0.0	0.0	11.305147058823529	0.0
31	0.0	0.0	0.0	11.695772058823529	0.0
32	0.0	0.0	0.0	12.068342962184873	0.0
33	0.0	0.0	0.0	12.450761554621849	0.0
34	0.001641281512605042	0.0	0.0	12.87749474789916	0.0
35	0.001641281512605042	0.0	0.0	13.296021533613445	0.0
36	0.001641281512605042	0.0	0.0	13.801536239495798	0.0
37	0.001641281512605042	0.0	0.0	14.202008928571429	0.0
38	0.001641281512605042	0.0	0.0	14.645154936974789	0.0
39	0.001641281512605042	0.0	0.0	15.135898109243698	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACTAA	45	3.7289283E-10	45.000004	8
TTGCGGG	30	2.1317046E-6	45.000004	3
CGGTTTA	45	3.7289283E-10	45.000004	1
TAAGGGC	30	2.1317046E-6	45.000004	4
GATGGCC	20	6.9807336E-4	45.0	9
GAGCGGG	25	3.8473132E-5	45.0	3
TGCACCA	25	3.8473132E-5	45.0	28
TATGCCC	25	3.8473132E-5	45.0	10
TACGGGA	20	6.9807336E-4	45.0	4
CTAAGGG	20	6.9807336E-4	45.0	3
TCGTGTA	25	3.8473132E-5	45.0	17
AGGGACC	25	3.8473132E-5	45.0	6
CACCTTC	25	3.8473132E-5	45.0	33
AGAGCGG	25	3.8473132E-5	45.0	2
CGGGATT	20	6.9807336E-4	45.0	6
ATGCCCT	25	3.8473132E-5	45.0	11
GGGACTA	40	6.6338544E-9	45.0	7
CCATAGC	20	6.9807336E-4	45.0	35
GGGAATC	25	3.8473132E-5	45.0	7
GTGCGGG	20	6.9807336E-4	45.0	3
>>END_MODULE