FastQCFastQC Report
Sat 18 Jun 2016
SRR3549451_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549451_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223136
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46462.0821382475261725No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19270.8635988813996844No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCG12380.554818585974473No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTC12010.5382367704001148No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGC9120.4087193460490463No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5410.24245303312777858No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5260.23573067546249823No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.21332281657823032No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTC3830.17164419905349204No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCT3550.15909579807830201No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT3410.15282159759070701No Hit
CCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC3230.14475476839237056No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA2720.12189875233041732No Hit
GCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC2680.1201061236196759No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCT207.0210535E-445.0000046
ACACGGG207.0210535E-445.0000043
CAACGCA207.0210535E-445.00000417
TGGACGG207.0210535E-445.0000042
TGGGTCC406.7720975E-945.0000046
GTCTCGT406.7720975E-945.00000427
ATAGCGG351.2060445E-745.0000042
CCTCGGG207.0210535E-445.0000043
ACGGGTA207.0210535E-445.0000045
ACGGGGC207.0210535E-445.0000045
TATTGCG351.2060445E-745.0000041
GGGACCG700.045.0000047
ACAGGCG207.0210535E-445.00000433
CCTCCGC207.0210535E-445.00000445
AACGCAA207.0210535E-445.00000418
GCCTCCG207.0210535E-445.00000444
GTACTCC207.0210535E-445.00000421
CAAGCGA207.0210535E-445.00000415
TATAGCG207.0210535E-445.0000041
CGCACGC207.0210535E-445.00000425