##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549451_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	223136
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.064086476409006	31.0	30.0	33.0	28.0	34.0
2	31.049283844830057	31.0	31.0	34.0	28.0	34.0
3	29.936635056647066	31.0	30.0	34.0	25.0	34.0
4	34.65733902194177	35.0	35.0	37.0	32.0	37.0
5	34.77253782446579	35.0	35.0	37.0	32.0	37.0
6	34.86047522587122	35.0	35.0	37.0	32.0	37.0
7	31.98845098953105	35.0	35.0	37.0	17.0	37.0
8	33.52971730245232	35.0	35.0	37.0	19.0	37.0
9	36.50587982217123	39.0	35.0	39.0	32.0	39.0
10	36.25096353793202	38.0	35.0	39.0	32.0	39.0
11	36.20471819876667	38.0	35.0	39.0	32.0	39.0
12	36.19282858884268	37.0	35.0	39.0	32.0	39.0
13	32.77756614799943	37.0	33.0	39.0	15.0	39.0
14	35.11464756919547	38.0	34.0	40.0	18.0	41.0
15	36.3742067617955	38.0	34.0	40.0	30.0	41.0
16	36.9483229958411	38.0	35.0	40.0	32.0	41.0
17	37.05684425641761	38.0	35.0	40.0	32.0	41.0
18	36.8712623691381	38.0	36.0	40.0	32.0	41.0
19	36.71649576939624	38.0	35.0	40.0	31.0	41.0
20	36.442003979635736	38.0	35.0	40.0	31.0	41.0
21	36.36581277785745	38.0	35.0	40.0	31.0	41.0
22	36.37729456474975	38.0	35.0	40.0	31.0	41.0
23	36.25196292843826	37.0	35.0	40.0	31.0	41.0
24	36.03016545963	37.0	34.0	40.0	31.0	41.0
25	35.84485694822888	37.0	34.0	40.0	30.0	41.0
26	35.6874417395669	37.0	34.0	40.0	30.0	41.0
27	35.68457801520149	37.0	34.0	40.0	30.0	41.0
28	35.60325093216693	37.0	34.0	40.0	30.0	41.0
29	35.55682184855873	37.0	34.0	40.0	30.0	41.0
30	35.31751936039008	37.0	34.0	40.0	29.0	41.0
31	35.07991090635308	37.0	34.0	40.0	27.0	41.0
32	34.71898304173239	37.0	33.0	40.0	25.0	41.0
33	34.26508945217267	37.0	33.0	40.0	23.0	41.0
34	33.814364333859174	37.0	33.0	40.0	21.0	41.0
35	33.336745303312775	37.0	33.0	40.0	18.0	41.0
36	32.989862684640755	36.0	32.0	40.0	15.0	41.0
37	32.76012387064391	36.0	32.0	40.0	15.0	41.0
38	32.51537627276638	36.0	31.0	40.0	12.0	41.0
39	32.444132726229746	36.0	31.0	40.0	10.0	41.0
40	32.3013677757063	36.0	31.0	40.0	10.0	41.0
41	32.199528538649076	36.0	31.0	40.0	10.0	41.0
42	32.174418291983365	36.0	31.0	40.0	10.0	41.0
43	32.19082532625843	36.0	31.0	40.0	10.0	41.0
44	32.005615409436395	36.0	30.0	40.0	10.0	41.0
45	32.011047074429946	36.0	30.0	40.0	10.0	41.0
46	31.870809730388643	35.0	30.0	40.0	10.0	41.0
47	31.6777615445289	35.0	30.0	39.0	10.0	40.0
48	31.532056682919833	35.0	30.0	39.0	10.0	40.0
49	31.495433278359386	35.0	29.0	39.0	9.0	40.0
50	31.302801878674888	35.0	29.0	39.0	9.0	40.0
51	29.41811272049333	34.0	24.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	10.0
11	8.0
12	3.0
13	5.0
14	12.0
15	14.0
16	32.0
17	88.0
18	191.0
19	393.0
20	680.0
21	1138.0
22	1747.0
23	2750.0
24	4239.0
25	6036.0
26	6920.0
27	6938.0
28	6516.0
29	6341.0
30	6958.0
31	8807.0
32	10784.0
33	14198.0
34	19278.0
35	22579.0
36	23198.0
37	28488.0
38	28577.0
39	16203.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.519252832353363	23.67928079736125	27.115750035852574	22.68571633443281
2	37.65819948372293	27.197762799368995	23.982235049476554	11.161802667431521
3	27.362236483579522	26.394665136956835	34.17646637028538	12.066632009178258
4	21.598487021368136	23.785942205650365	41.29096156604044	13.324609206941057
5	20.95134805679048	27.750788756632726	38.31295712032124	12.984906066255558
6	17.718790334145993	36.88736913810411	36.37602179836512	9.01781872938477
7	63.480119747597875	14.250950093216694	19.782554137387066	2.4863760217983653
8	69.01441273483437	6.915513408862756	20.17962139681629	3.890452459486591
9	64.39704933314212	7.568030259572637	21.75041230460347	6.284508102681772
10	33.043525025096805	25.150580811702277	29.00921411157321	12.796680051627707
11	25.44188297719776	24.6907715473971	34.76713753047469	15.100207944930446
12	23.11370643912233	21.68677398537215	39.59513480567905	15.604384769826474
13	16.811720206510827	34.18363688512835	37.22752043596731	11.777122472393518
14	15.328319948372293	31.337390649648643	38.31833500645347	15.015954395525599
15	14.089613509249965	25.551681485730676	46.82346192456618	13.535243080453178
16	15.079144557579236	25.790101104259282	42.59733973899326	16.53341459916822
17	15.353416750322674	25.738563028825467	37.63399899612792	21.274021224723935
18	17.056414025527033	26.405869066398967	39.83803599598451	16.699680912089487
19	18.588215258855588	29.01414384052775	36.070378603183705	16.327262297432956
20	19.48184067116019	27.412878244657968	37.956224006883694	15.149057077298151
21	16.60556790477556	29.247633730101818	38.70330202208518	15.44349634303743
22	16.3259178258999	25.814301591854296	36.98910081743869	20.87067976480711
23	14.941112146852145	29.129320235192885	37.01464577656676	18.91492184138821
24	16.341155169941203	26.730783020220848	39.16938548687796	17.75867632295999
25	14.139807113150724	30.06372795066686	37.26471748171519	18.531747454467233
26	13.92693245375018	30.55804531765381	37.27816219704575	18.236860031550265
27	16.120661838520007	29.188925139825038	37.40185357808691	17.28855944356805
28	14.1160547827334	27.2649863760218	40.65099311630575	17.96796572493905
29	14.75512691811272	25.565126201061233	38.57781801233329	21.10192886849276
30	16.313817582102395	27.90316219704575	38.538380180696976	17.24464004015488
31	19.723397389932597	27.489961279219848	33.918775993116306	18.86786533773125
32	18.329628567331135	27.757511114298005	34.53185501218988	19.381005306180985
33	17.06448085472537	26.82086261293561	35.867811558869924	20.246844973469095
34	15.674297289545388	27.31293919403413	34.726803384483006	22.285960131937475
35	15.150401548831205	26.82175892729098	34.909651512978634	23.118188010899182
36	18.571633443281225	26.507600745733544	35.9758174386921	18.94494837229313
37	16.998601749605623	27.41735981643482	35.80506955399398	19.778968879965582
38	16.73732611501506	29.53938405277499	32.55772264448588	21.16556718772408
39	17.8563745876954	26.602161910225153	34.248619675892726	21.29284382618672
40	17.570898465509824	27.620823175103972	33.68214900329844	21.126129356087766
41	15.488312060805967	27.77723003011616	33.36843897891868	23.366018930159186
42	17.919116592571346	27.11888713609637	34.44401620536354	20.517980065968736
43	18.976319374731105	25.66192815144127	34.335114011186	21.026638462641618
44	17.93928366556719	27.41691165925714	32.14407356948229	22.49973110569339
45	17.102126057650942	27.832801520149147	31.29795281801233	23.76711960418758
46	19.786139394808547	28.296644199053496	31.02009536784741	20.89712103829055
47	15.856249103685643	26.54524594865911	35.44968091208949	22.148824035565752
48	17.041624838663417	26.320271045461062	34.1760182131077	22.46208590276782
49	17.959002581385345	23.91994120177829	35.65045532769253	22.470600889143842
50	16.229115875519863	25.306539509536783	34.775204359673026	23.689140255270328
51	15.892997992255845	24.95383981069841	31.724598451168795	27.428563745876954
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	618.0
1	593.5
2	569.0
3	4015.0
4	7461.0
5	6579.5
6	5698.0
7	4502.0
8	3306.0
9	3298.0
10	3290.0
11	3318.0
12	3346.0
13	3362.0
14	3378.0
15	3177.0
16	2976.0
17	2882.0
18	2788.0
19	2608.5
20	2429.0
21	2322.5
22	2216.0
23	2231.5
24	2247.0
25	2438.5
26	2769.5
27	2909.0
28	3213.5
29	3518.0
30	4059.5
31	4601.0
32	4979.0
33	5357.0
34	5880.5
35	6404.0
36	7067.0
37	7730.0
38	8664.5
39	9599.0
40	11083.0
41	12567.0
42	14221.5
43	15876.0
44	17392.5
45	18909.0
46	22612.0
47	26315.0
48	24024.0
49	21733.0
50	19615.0
51	17497.0
52	14356.5
53	11216.0
54	9069.5
55	6923.0
56	5384.5
57	3846.0
58	3063.0
59	2280.0
60	1805.5
61	1331.0
62	1039.0
63	747.0
64	559.0
65	371.0
66	284.0
67	197.0
68	152.0
69	107.0
70	83.5
71	60.0
72	46.0
73	32.0
74	30.0
75	21.5
76	15.0
77	12.0
78	9.0
79	5.5
80	2.0
81	2.0
82	2.0
83	2.0
84	2.0
85	1.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	223136.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.213521847981816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.24659778270686	40.33316921009628
2	10.711514853844852	11.185718296924199
3	3.8946034846536866	6.100528924055741
4	2.001482518435549	4.180178048187528
5	1.3315634555909672	3.476280879023656
6	0.9760887727568337	3.0579019477145217
7	0.732050128796566	2.675604077561618
8	0.6121258045928184	2.5568995257456457
9	0.46551922216544533	2.187575826947188
>10	1.9680362043449653	15.029984880172112
>50	0.028723203134957864	1.0177543178760304
>100	0.02476138201289471	2.514391002801453
>500	0.0029713658415473653	1.0234429853031828
>1k	0.003961821122063154	4.6605700775908465
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4646	2.0821382475261725	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1927	0.8635988813996844	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCG	1238	0.554818585974473	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTC	1201	0.5382367704001148	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGC	912	0.4087193460490463	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	541	0.24245303312777858	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	526	0.23573067546249823	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	476	0.21332281657823032	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTC	383	0.17164419905349204	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCT	355	0.15909579807830201	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT	341	0.15282159759070701	No Hit
CCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	323	0.14475476839237056	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	272	0.12189875233041732	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	268	0.1201061236196759	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10710956546680052	0.0
2	0.0	0.0	0.0	0.5956008891438406	0.0
3	0.0	0.0	0.0	0.915585114011186	0.0
4	0.0	0.0	0.0	1.533145704861609	0.0
5	0.0	0.0	0.0	3.1819159615660406	0.0
6	0.0	0.0	0.0	4.055822458052488	0.0
7	0.0	0.0	0.0	4.791696543811845	0.0
8	0.0	0.0	0.0	6.2128029542521155	0.0
9	0.0	0.0	0.0	6.702638749462212	0.0
10	0.0	0.0	0.0	8.375609493761653	0.0
11	0.0	0.0	0.0	10.054854438548688	0.0
12	0.0	0.0	0.0	11.748440413021655	0.0
13	0.0	0.0	0.0	12.314462928438262	0.0
14	0.0	0.0	0.0	12.518822601462785	0.0
15	0.0	0.0	0.0	12.927093790334146	0.0
16	0.0	0.0	0.0	13.712265165638893	0.0
17	0.0	0.0	0.0	14.581241933170801	0.0
18	0.0	0.0	0.0	15.7504840097519	0.0
19	0.0	0.0	0.0	16.411964004015488	0.0
20	0.0	0.0	0.0	17.06627348343611	0.0
21	0.0	0.0	0.0	17.84561881543095	0.0
22	0.0	0.0	0.0	18.663953821884412	0.0
23	0.0	0.0	0.0	19.548616090635306	0.0
24	0.0	0.0	0.0	20.200236626989817	0.0
25	0.0	0.0	0.0	20.781944643625412	0.0
26	0.0	0.0	0.0	21.266402552703283	0.0
27	0.0	0.0	0.0	21.7862648788183	0.0
28	0.0	0.0	0.0	22.35183923705722	0.0
29	0.0	0.0	0.0	22.97925928581672	0.0
30	0.0	0.0	0.0	23.67838448300588	0.0
31	0.0	0.0	0.0	24.326419761938908	0.0
32	0.0	0.0	0.0	24.933224580524882	0.0
33	0.0	0.0	0.0	25.52524021224724	0.0
34	0.0	0.0	0.0	26.159830775849706	0.0
35	0.0	0.0	0.0	26.80338448300588	0.0
36	0.0	0.0	0.0	27.434389789186863	0.0
37	0.0	0.0	0.0	28.046572493905064	0.0
38	0.0	0.0	0.0	28.649792055069554	0.0
39	0.0	0.0	0.0	29.310823892155458	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCT	20	7.0210535E-4	45.000004	6
ACACGGG	20	7.0210535E-4	45.000004	3
CAACGCA	20	7.0210535E-4	45.000004	17
TGGACGG	20	7.0210535E-4	45.000004	2
TGGGTCC	40	6.7720975E-9	45.000004	6
GTCTCGT	40	6.7720975E-9	45.000004	27
ATAGCGG	35	1.2060445E-7	45.000004	2
CCTCGGG	20	7.0210535E-4	45.000004	3
ACGGGTA	20	7.0210535E-4	45.000004	5
ACGGGGC	20	7.0210535E-4	45.000004	5
TATTGCG	35	1.2060445E-7	45.000004	1
GGGACCG	70	0.0	45.000004	7
ACAGGCG	20	7.0210535E-4	45.000004	33
CCTCCGC	20	7.0210535E-4	45.000004	45
AACGCAA	20	7.0210535E-4	45.000004	18
GCCTCCG	20	7.0210535E-4	45.000004	44
GTACTCC	20	7.0210535E-4	45.000004	21
CAAGCGA	20	7.0210535E-4	45.000004	15
TATAGCG	20	7.0210535E-4	45.000004	1
CGCACGC	20	7.0210535E-4	45.000004	25
>>END_MODULE