FastQCFastQC Report
Sat 18 Jun 2016
SRR3549449_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549449_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415847
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82751.989914559922279No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35920.8637792264943597No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC26540.6382154975267346No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG23600.567516418298076No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC20280.4876793628425839No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9820.23614454354606382No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9040.21738764497519522No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8610.20704730345535738No Hit
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT8000.1923784468807037Illumina PCR Primer Index 8 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC7460.17939290171625621RNA PCR Primer, Index 40 (96% over 27bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTC7150.17193823689962895RNA PCR Primer, Index 40 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCT6360.15294086527015946No Hit
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC6180.14861235021534364Illumina PCR Primer Index 8 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCT5960.14332194292612427RNA PCR Primer, Index 40 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTA5060.12167936765204511No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA4550.10941524166340023No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4330.1041248343741809No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACG351.2094279E-745.0000041
CGCGGGC207.028086E-445.0000044
GCGCGGA207.028086E-445.0000044
TACGGGT351.2094279E-745.0000044
CGACAAT207.028086E-445.00000420
TCTACGG406.7975634E-945.0000042
GCGATGC207.028086E-445.0000049
GCCACGG700.045.0000041
CGATATA207.028086E-445.00000410
TCACGGG551.8189894E-1245.03
CGGCAGT253.886497E-545.09
TTCACGG253.886497E-545.02
TGAGTAG453.8380676E-1045.01
CGCGAGG253.886497E-545.02
TGCGGGT453.8380676E-1045.04
ACGGGCC253.886497E-545.05
AATTGCG253.886497E-545.01
CGTCCTT253.886497E-545.045
CGGGTCA302.1621472E-644.9999966
GGTACTC302.1621472E-644.9999968
Produced by FastQC (version 0.11.5)