##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549449_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	415847
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07763191750812	31.0	30.0	33.0	28.0	34.0
2	31.038932588187482	31.0	31.0	34.0	28.0	34.0
3	29.92240896291184	31.0	30.0	34.0	25.0	34.0
4	34.66506912398069	35.0	35.0	37.0	32.0	37.0
5	34.785216678249455	35.0	35.0	37.0	32.0	37.0
6	34.880511341911806	35.0	35.0	37.0	32.0	37.0
7	32.06327567590965	35.0	35.0	37.0	18.0	37.0
8	33.53078175386621	35.0	35.0	37.0	19.0	37.0
9	36.53599761450726	39.0	35.0	39.0	32.0	39.0
10	36.266617289531965	38.0	35.0	39.0	32.0	39.0
11	36.2657179202928	38.0	35.0	39.0	32.0	39.0
12	36.34254906251578	38.0	35.0	39.0	32.0	39.0
13	32.91764278689037	37.0	34.0	39.0	15.0	39.0
14	35.33806904943405	38.0	34.0	40.0	18.0	41.0
15	36.541670373959654	38.0	34.0	40.0	30.0	41.0
16	37.124988276938396	39.0	36.0	40.0	32.0	41.0
17	37.21706300634608	39.0	36.0	40.0	32.0	41.0
18	37.05320947367662	39.0	36.0	40.0	32.0	41.0
19	36.895730881790655	38.0	36.0	40.0	32.0	41.0
20	36.63585886155244	38.0	35.0	40.0	32.0	41.0
21	36.591847482367314	38.0	35.0	40.0	31.0	41.0
22	36.62575899309121	38.0	35.0	40.0	32.0	41.0
23	36.47023785190226	38.0	35.0	40.0	31.0	41.0
24	36.28326283464832	38.0	35.0	40.0	31.0	41.0
25	36.095965583495854	38.0	35.0	40.0	31.0	41.0
26	35.99938919843115	38.0	35.0	40.0	31.0	41.0
27	35.99564503290874	38.0	35.0	40.0	30.0	41.0
28	35.8742229714294	37.0	34.0	40.0	30.0	41.0
29	35.81453274882349	37.0	34.0	40.0	30.0	41.0
30	35.543348875908684	37.0	34.0	40.0	29.0	41.0
31	35.29507968074797	37.0	34.0	40.0	27.0	41.0
32	34.95974961945139	37.0	34.0	40.0	25.0	41.0
33	34.4359079180564	37.0	33.0	40.0	24.0	41.0
34	33.899515927733034	37.0	33.0	40.0	21.0	41.0
35	33.51383080796543	37.0	33.0	40.0	18.0	41.0
36	33.044473087457646	37.0	32.0	40.0	15.0	41.0
37	32.81377525868889	37.0	31.0	40.0	13.0	41.0
38	32.54489992713666	37.0	31.0	40.0	10.0	41.0
39	32.233422388522705	36.0	30.0	40.0	10.0	41.0
40	32.071519092358486	36.0	30.0	40.0	10.0	41.0
41	32.027079671129044	36.0	30.0	40.0	10.0	41.0
42	31.964813982065518	36.0	30.0	40.0	10.0	41.0
43	31.870416282911744	36.0	30.0	40.0	9.0	41.0
44	31.655091896779343	36.0	30.0	40.0	9.0	41.0
45	31.606879453260454	36.0	30.0	40.0	9.0	41.0
46	31.31805207203611	35.0	28.0	40.0	8.0	41.0
47	31.01999773955325	35.0	27.0	39.0	8.0	40.0
48	30.91030595387246	35.0	27.0	39.0	8.0	40.0
49	30.79888276216974	35.0	27.0	39.0	8.0	40.0
50	30.58607131949972	35.0	26.0	39.0	8.0	40.0
51	28.647331831178295	33.0	22.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	9.0
9	8.0
10	13.0
11	9.0
12	10.0
13	4.0
14	14.0
15	16.0
16	53.0
17	160.0
18	319.0
19	578.0
20	1147.0
21	1967.0
22	3184.0
23	5192.0
24	8179.0
25	11239.0
26	13742.0
27	13492.0
28	12702.0
29	12664.0
30	13815.0
31	16090.0
32	19780.0
33	25244.0
34	33199.0
35	39921.0
36	43938.0
37	53841.0
38	54370.0
39	30946.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.333286040298475	20.483495131622927	24.993567345682425	22.18965148239617
2	36.75245943820684	27.551719743078586	24.345011506635856	11.350809312078722
3	27.52166061075347	27.20952658068953	33.64747130555229	11.621341503004711
4	20.661685668046182	23.77725461527918	43.16900206085411	12.39205765582053
5	21.325391309784607	27.274694779570368	37.80212433899968	13.597789571645341
6	16.740051028383036	37.2603385379719	36.718793210002715	9.280817223642348
7	60.73123047659356	13.946235033558017	21.999677766101474	3.322856723746955
8	64.39243279379194	8.259047197647211	21.925612064052405	5.422907944508437
9	60.34599263671494	7.158642481489587	24.144937921879922	8.350426959915545
10	30.679793289358827	23.128939249291207	32.19116646266536	14.000100998684612
11	22.719654103552507	25.48653711581423	35.11820453195526	16.675604248678
12	19.785401842504573	21.6786462328693	40.811163721272486	17.724788203353636
13	15.87458849047847	32.4225015450394	39.57849882288438	12.12441114159775
14	15.942642366062518	29.38989580302371	38.47568937614074	16.19177245477303
15	13.733175903637637	26.835590974565164	45.605956036715426	13.825277085081773
16	14.08474751531212	26.095414900191656	41.002820748977385	18.81701683551883
17	14.215083913073798	26.718240121967934	38.7914305020837	20.275245462874565
18	14.857146979538147	27.125601483237826	40.76859998990013	17.248651547323895
19	16.81748335325252	29.34348450271374	36.729374024581155	17.109658119452586
20	17.409287550469283	27.184998328712247	39.81488383948904	15.590830281329431
21	15.944806623589928	29.93216255016869	38.937157175595836	15.18587365064555
22	14.659959071485426	25.3417723345365	37.554918034757975	22.4433505592201
23	14.514953817149095	29.566883974153956	37.506102003862	18.41206020483495
24	16.303111480905237	25.896784153787326	38.41292590784591	19.387178457461516
25	13.702395352136723	30.369342570705093	36.970568502357835	18.957693574800345
26	13.52059771983446	27.90858176204229	39.286805002801515	19.28401551532174
27	16.68498269796343	28.310171769905757	36.299889141919984	18.70495639021082
28	12.93360298378971	27.915074534624512	40.2119048592391	18.93941762234668
29	15.65888415691348	24.9041113678829	38.80177084360354	20.635233631600084
30	15.408070756792764	29.23527162634334	36.896983746425974	18.459673870437925
31	16.201150904058466	30.378480546931925	34.02501400755567	19.395354541453948
32	17.600463632056982	28.689878729436547	34.924864192840154	18.784793445666313
33	15.495122004006282	29.333384634252504	32.952985112312945	22.218508249428275
34	14.477440020007359	30.348180941548215	35.043417410730385	20.13096162771404
35	15.496564842357888	26.097579157719064	33.070576437968775	25.335279561954277
36	15.226273124490497	29.77513364290232	33.03186027553403	21.966732957073155
37	15.471074698146193	30.11756727834997	33.24828602827482	21.163071995229014
38	15.971018186977423	31.19921509593673	29.76262904385507	23.067137673230782
39	19.10582497889849	28.951753890252906	31.478163843913748	20.46425728693486
40	16.84537822805022	28.570123146253312	32.943847136086106	21.64065148961036
41	14.220133847304417	28.884902379961865	30.976777516730913	25.918186256002805
42	16.90068703152842	26.14591424249784	33.17614411069456	23.77725461527918
43	17.760137742967967	25.42593790504681	32.91066185399919	23.903262497986038
44	16.74630332790666	27.625304499010454	31.99013098567502	23.63826118740787
45	16.71047284217513	26.82404826775232	32.13633860530435	24.329140284768197
46	19.07937294245239	28.008137608303052	31.21580773698019	21.696681712264365
47	14.566655524748285	26.178137632350364	35.74006786149714	23.515138981404217
48	15.407589810675562	26.404182307435185	33.169410865053734	25.01881701683552
49	16.58254117499946	24.53498522293055	35.50921372524029	23.3732598768297
50	15.970056294743017	24.862990474862148	34.38379981098817	24.783153419406656
51	14.892977465269677	24.64464093765255	31.920874744797967	28.541506852279802
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1048.0
1	1010.5
2	973.0
3	7507.0
4	14041.0
5	12598.5
6	11156.0
7	8837.5
8	6519.0
9	6392.0
10	6265.0
11	6220.0
12	6175.0
13	6073.5
14	5972.0
15	5607.5
16	5243.0
17	5154.5
18	5066.0
19	4802.5
20	4539.0
21	4446.0
22	4353.0
23	4234.5
24	4116.0
25	4549.0
26	5481.5
27	5981.0
28	6306.0
29	6631.0
30	7517.0
31	8403.0
32	9171.0
33	9939.0
34	10945.0
35	11951.0
36	12913.0
37	13875.0
38	15583.5
39	17292.0
40	20042.0
41	22792.0
42	25384.0
43	27976.0
44	31942.5
45	35909.0
46	44074.0
47	52239.0
48	47783.5
49	43328.0
50	38453.5
51	33579.0
52	26839.5
53	20100.0
54	15571.5
55	11043.0
56	8538.0
57	6033.0
58	4841.0
59	3649.0
60	2911.0
61	2173.0
62	1665.0
63	1157.0
64	879.0
65	601.0
66	451.0
67	301.0
68	232.0
69	163.0
70	152.0
71	141.0
72	100.5
73	60.0
74	47.5
75	27.0
76	19.0
77	20.0
78	21.0
79	12.0
80	3.0
81	2.0
82	1.0
83	1.0
84	1.0
85	1.5
86	2.0
87	1.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	415847.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.28486364223148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.23302725635422	53.939605077329766
2	7.580359582107514	9.594440450255137
3	2.49835041218621	4.743232954971513
4	1.2344283799764615	3.1248252681164432
5	0.7996287357146051	2.5302197752054374
6	0.5761428135844593	2.187667163768646
7	0.45005241294004994	1.9937053909337823
8	0.32218186912063035	1.631138852423869
9	0.2578116459092021	1.4684017371068807
>10	0.9985970704351967	9.638865381894318
>50	0.029354630923521828	1.2110347655357103
>100	0.01449152665120454	1.7587046616972941
>500	0.00371577606441142	1.7316621378177393
>1k	0.0014863104257645682	2.500610425523335
>5k	3.7157760644114204E-4	1.9458859574201242
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8275	1.989914559922279	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3592	0.8637792264943597	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	2654	0.6382154975267346	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	2360	0.567516418298076	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	2028	0.4876793628425839	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	982	0.23614454354606382	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	904	0.21738764497519522	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	861	0.20704730345535738	No Hit
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT	800	0.1923784468807037	Illumina PCR Primer Index 8 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	746	0.17939290171625621	RNA PCR Primer, Index 40 (96% over 27bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTC	715	0.17193823689962895	RNA PCR Primer, Index 40 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCT	636	0.15294086527015946	No Hit
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	618	0.14861235021534364	Illumina PCR Primer Index 8 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCT	596	0.14332194292612427	RNA PCR Primer, Index 40 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTA	506	0.12167936765204511	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	455	0.10941524166340023	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	433	0.1041248343741809	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.809461172017593E-4	0.0	0.0	0.5242312677499177	0.0
2	4.809461172017593E-4	0.0	0.0	1.5416727786902393	0.0
3	4.809461172017593E-4	0.0	0.0	1.9757266494648271	0.0
4	4.809461172017593E-4	0.0	0.0	2.926797596231306	0.0
5	4.809461172017593E-4	0.0	0.0	5.244236461967984	0.0
6	4.809461172017593E-4	0.0	0.0	6.323239075910131	0.0
7	4.809461172017593E-4	0.0	0.0	7.317354700166167	0.0
8	4.809461172017593E-4	0.0	0.0	9.342859272761377	0.0
9	4.809461172017593E-4	0.0	0.0	9.891859265547184	0.0
10	4.809461172017593E-4	0.0	0.0	12.030386175684807	0.0
11	4.809461172017593E-4	0.0	0.0	13.912809278412492	0.0
12	4.809461172017593E-4	0.0	0.0	16.292530666326797	0.0
13	4.809461172017593E-4	0.0	0.0	16.97282894910869	0.0
14	4.809461172017593E-4	0.0	0.0	17.250334858734103	0.0
15	4.809461172017593E-4	0.0	0.0	17.8241035765558	0.0
16	4.809461172017593E-4	0.0	0.0	18.795855206361956	0.0
17	4.809461172017593E-4	0.0	0.0	19.905157425687815	0.0
18	4.809461172017593E-4	0.0	0.0	21.15104834229897	0.0
19	4.809461172017593E-4	0.0	0.0	21.934028621103433	0.0
20	4.809461172017593E-4	0.0	0.0	22.75067512811202	0.0
21	4.809461172017593E-4	0.0	0.0	23.789999687385023	0.0
22	4.809461172017593E-4	0.0	0.0	24.877899804495403	0.0
23	4.809461172017593E-4	0.0	0.0	26.036979946951643	0.0
24	4.809461172017593E-4	0.0	0.0	26.801684273302442	0.0
25	4.809461172017593E-4	0.0	0.0	27.49857519712779	0.0
26	4.809461172017593E-4	0.0	0.0	28.098074532219783	0.0
27	4.809461172017593E-4	0.0	0.0	28.718975969527254	0.0
28	4.809461172017593E-4	0.0	0.0	29.410095539946184	0.0
29	4.809461172017593E-4	0.0	0.0	30.129109985162813	0.0
30	4.809461172017593E-4	0.0	0.0	30.895738095982416	0.0
31	4.809461172017593E-4	0.0	0.0	31.660923368450415	0.0
32	4.809461172017593E-4	0.0	0.0	32.40085896976532	0.0
33	4.809461172017593E-4	0.0	0.0	33.14945160118986	0.0
34	4.809461172017593E-4	0.0	0.0	33.84946867477702	0.0
35	4.809461172017593E-4	0.0	0.0	34.57209021587266	0.0
36	4.809461172017593E-4	0.0	0.0	35.291345134147896	0.0
37	4.809461172017593E-4	0.0	0.0	36.007714375719914	0.0
38	4.809461172017593E-4	0.0	0.0	36.74716903091762	0.0
39	4.809461172017593E-4	0.0	0.0	37.46546205695845	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCTACG	35	1.2094279E-7	45.000004	1
CGCGGGC	20	7.028086E-4	45.000004	4
GCGCGGA	20	7.028086E-4	45.000004	4
TACGGGT	35	1.2094279E-7	45.000004	4
CGACAAT	20	7.028086E-4	45.000004	20
TCTACGG	40	6.7975634E-9	45.000004	2
GCGATGC	20	7.028086E-4	45.000004	9
GCCACGG	70	0.0	45.000004	1
CGATATA	20	7.028086E-4	45.000004	10
TCACGGG	55	1.8189894E-12	45.0	3
CGGCAGT	25	3.886497E-5	45.0	9
TTCACGG	25	3.886497E-5	45.0	2
TGAGTAG	45	3.8380676E-10	45.0	1
CGCGAGG	25	3.886497E-5	45.0	2
TGCGGGT	45	3.8380676E-10	45.0	4
ACGGGCC	25	3.886497E-5	45.0	5
AATTGCG	25	3.886497E-5	45.0	1
CGTCCTT	25	3.886497E-5	45.0	45
CGGGTCA	30	2.1621472E-6	44.999996	6
GGTACTC	30	2.1621472E-6	44.999996	8
>>END_MODULE