FastQCFastQC Report
Sat 18 Jun 2016
SRR3549442_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549442_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128902
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23801.8463639043614528No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.7439760438162325No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG8060.6252812213929962No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC6980.5414966408589471No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC5100.3956494080774542No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA3260.2529053079083335No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3250.2521295247552404No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3020.23428651223410032No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2690.20868566818202974No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.19860048719182014No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG2250.17455120944593566No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC1950.15127771485314426No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCT1630.12645265395416674No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1630.12645265395416674No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTC1460.11326434035158492No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT1380.10705807512684054No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG1360.10550650882065445No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC1350.1047307256675614No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATC253.8714956E-545.00000411
ATGGGCG502.1827873E-1145.0000045
CTGTCTA207.009974E-445.0000041
CACGGGC207.009974E-445.0000044
CAACGCA207.009974E-445.00000417
TTAGCGG207.009974E-445.0000042
ACGCAAG207.009974E-445.00000419
GGGTCCA253.8714956E-545.0000047
ATGGATT207.009974E-445.00000416
TTAGATG207.009974E-445.0000041
CGGTTTA1000.045.0000041
TATGGCC207.009974E-445.00000410
CTGAGAG207.009974E-445.00000419
AACCAGA207.009974E-445.00000410
ATAGCCC207.009974E-445.00000431
GTTAGCG207.009974E-445.0000041
GGATATC207.009974E-445.0000048
CACGAGA207.009974E-445.00000414
CCTCGTC302.1504802E-645.00000436
ACTTACT207.009974E-445.00000438