##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549442_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	128902
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39876805635289	31.0	31.0	34.0	30.0	34.0
2	31.429279607764038	31.0	31.0	34.0	30.0	34.0
3	30.575848318877906	31.0	31.0	34.0	26.0	34.0
4	35.0413104529022	37.0	35.0	37.0	32.0	37.0
5	35.09138725543436	35.0	35.0	37.0	32.0	37.0
6	35.18789467967913	36.0	35.0	37.0	32.0	37.0
7	32.09463002901429	36.0	35.0	37.0	16.0	37.0
8	33.63048672635025	37.0	35.0	37.0	19.0	37.0
9	36.70086577399885	39.0	35.0	39.0	32.0	39.0
10	36.62932305162061	38.0	35.0	39.0	32.0	39.0
11	36.51349086903229	38.0	35.0	39.0	32.0	39.0
12	36.16587795379436	37.0	35.0	39.0	32.0	39.0
13	32.28938263176677	37.0	33.0	39.0	8.0	39.0
14	34.70386029696979	38.0	33.0	40.0	17.0	41.0
15	36.185218227800966	38.0	34.0	40.0	29.0	41.0
16	36.91571116041644	38.0	35.0	40.0	32.0	41.0
17	37.071224651285476	38.0	35.0	40.0	32.0	41.0
18	36.975873143938806	38.0	35.0	40.0	32.0	41.0
19	36.850134210485486	38.0	35.0	40.0	32.0	41.0
20	36.6312780251664	38.0	35.0	40.0	32.0	41.0
21	36.559634450978265	38.0	35.0	40.0	32.0	41.0
22	36.56402538362477	38.0	35.0	40.0	32.0	41.0
23	36.47614466804239	38.0	35.0	40.0	32.0	41.0
24	36.27324634218243	37.0	35.0	40.0	31.0	41.0
25	36.11957921521776	37.0	35.0	40.0	31.0	41.0
26	35.94314285270981	37.0	35.0	40.0	31.0	41.0
27	35.92092442320523	37.0	34.0	40.0	31.0	41.0
28	35.883818714992785	37.0	34.0	40.0	31.0	41.0
29	35.85015748398008	37.0	35.0	40.0	31.0	41.0
30	35.654279995655614	36.0	34.0	40.0	30.0	41.0
31	35.418247971327055	36.0	34.0	40.0	30.0	41.0
32	35.07485531644195	36.0	34.0	40.0	27.0	41.0
33	34.70950024049278	36.0	34.0	40.0	25.0	41.0
34	34.35402088408248	36.0	34.0	40.0	23.0	41.0
35	34.05466943879847	36.0	33.0	40.0	21.0	41.0
36	33.67751470109075	36.0	33.0	40.0	19.0	41.0
37	33.4909466106034	36.0	33.0	40.0	18.0	41.0
38	33.37755814494732	36.0	33.0	40.0	18.0	41.0
39	33.274262618112985	36.0	33.0	40.0	17.0	41.0
40	33.14086670493864	36.0	33.0	40.0	16.0	41.0
41	33.094304200089994	36.0	33.0	40.0	15.0	41.0
42	33.03909171308436	36.0	32.0	40.0	15.0	41.0
43	32.942312764736	35.0	32.0	40.0	15.0	41.0
44	32.69531116662271	35.0	32.0	40.0	15.0	41.0
45	32.65386883058447	35.0	32.0	40.0	15.0	41.0
46	32.580658174427086	35.0	31.0	40.0	14.0	41.0
47	32.37028905680284	35.0	31.0	40.0	13.0	41.0
48	32.29312190656468	35.0	31.0	39.0	12.0	41.0
49	32.286923399171464	35.0	31.0	39.0	12.0	41.0
50	32.05248948813828	35.0	31.0	39.0	10.0	41.0
51	30.32783044483406	34.0	27.0	38.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	6.0
10	2.0
11	5.0
12	0.0
13	3.0
14	6.0
15	7.0
16	12.0
17	51.0
18	86.0
19	172.0
20	319.0
21	478.0
22	859.0
23	1318.0
24	1907.0
25	2699.0
26	3276.0
27	3382.0
28	3275.0
29	3344.0
30	3872.0
31	4823.0
32	6075.0
33	8893.0
34	12676.0
35	14675.0
36	11691.0
37	15346.0
38	16900.0
39	12740.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.72022156366852	22.691657227971636	24.835146079967725	20.752975128392112
2	34.769825138477295	28.042233634854384	25.338629346325114	11.849311880343206
3	28.89714666956292	26.932863725931327	32.42152953406464	11.74846007044111
4	23.76844424446479	25.448014770911236	38.56728367286776	12.216257311756218
5	22.76070192859692	29.641122713379158	34.18643620735132	13.411739150672606
6	18.689391941164608	39.29729561992832	32.88777520907356	9.125537229833517
7	65.77322306868784	15.245690524584568	16.688647189337637	2.2924392173899553
8	71.45195574932895	8.78031372670711	16.54279995655614	3.224930567407798
9	67.3178073264961	8.591022637352408	17.922918185908678	6.16825185024282
10	41.2631301298661	24.64585499061302	24.1353896758778	9.955625203643077
11	35.62706552264511	22.165676250174553	29.44795270825899	12.759305518921352
12	31.983987835720157	22.164900467021457	32.7939054475493	13.05720624970908
13	17.39228250919303	39.70225442584289	31.1872585374936	11.718204527470482
14	13.605684938945867	38.15301546911608	33.693038121984145	14.54826146995392
15	12.763960217839909	25.541884532435493	49.63770926750555	12.05644598221905
16	13.846177716404712	23.41546290980745	45.84102651626817	16.897332857519668
17	13.210811314021504	23.261857845495026	36.87685218227801	26.650478658205458
18	17.3953856418054	26.565142511365224	38.88768211509519	17.151789731734183
19	23.02369241749546	25.8312516485392	33.96844114133217	17.176614792633163
20	24.104358349754076	25.75444911638299	35.171680811779495	14.969511722083443
21	17.784828784658114	29.523203674109013	35.854369986501375	16.837597554731502
22	16.958619726614018	26.3331833485904	33.74346402693519	22.96473289786039
23	15.957083675970893	30.012722843710726	34.46416657615863	19.56602690415975
24	16.528060076647375	25.351817659927693	41.30424663698003	16.815875626444896
25	14.407068936090983	28.281950629160136	38.90164621185087	18.409334222898018
26	13.154179143845713	33.262478472017506	34.96687405936293	18.616468324773862
27	15.442739445470202	33.48202510434283	35.13289165412484	15.942343796062126
28	12.033948270779351	29.742750306434345	42.06218677755194	16.161114645234363
29	14.872538827946812	27.378163255806736	39.74957719818156	17.99972071806489
30	15.159578594591238	32.110440489674325	36.46568711113869	16.264293804595738
31	23.017486152270717	28.870770042357762	31.743495058261317	16.368248747110208
32	24.07255124047726	30.093404291632403	31.44171541170812	14.392329056182215
33	20.8546027214473	29.860669345704487	30.849792865898124	18.434935066950086
34	16.851561651487177	29.493723914291476	32.93820111402461	20.71651332019674
35	19.114521109059595	28.38590557167461	32.37498254487905	20.124590774386743
36	22.96473289786039	29.676808738421435	31.39827155513491	15.960186808583266
37	18.112985058416474	32.25706350560891	33.42073823524848	16.209213200726133
38	18.707234953685745	32.07708181409132	29.703961148779694	19.511722083443235
39	20.730477416952414	28.95145149027944	31.245442273975577	19.072628818792573
40	22.413151076011236	26.544972149384805	31.43473336333028	19.607143411273682
41	16.370576096569486	28.175668337186387	31.01425889435385	24.439496671890275
42	19.961676312237202	27.013545173853004	31.26173372019053	21.76304479371926
43	21.20370514033917	27.30523963941599	30.402165986563435	21.088889233681403
44	17.95472529518549	30.164776341716966	31.710912165831406	20.16958619726614
45	16.737521527982498	33.541760407131	28.72569859272936	20.995019472157143
46	20.79098850289367	32.38739507532855	28.612434252377778	18.20918216940001
47	17.915160354377743	28.088780624039966	33.45409691083148	20.541962110750802
48	20.02994522970939	26.977859148810722	33.52857209352842	19.46362352795147
49	18.70335603792028	25.888659601868085	34.83886983910258	20.56911452110906
50	17.258847806861027	29.16013715846147	32.428511582442475	21.15250345223503
51	17.07964189849653	29.699306449861133	29.551907650773458	23.669144000868876
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	388.0
1	395.0
2	402.0
3	2166.5
4	3931.0
5	3403.5
6	2876.0
7	2228.5
8	1581.0
9	1591.0
10	1601.0
11	1544.5
12	1488.0
13	1469.0
14	1450.0
15	1369.5
16	1289.0
17	1198.0
18	1107.0
19	1144.5
20	1182.0
21	1141.5
22	1101.0
23	1124.5
24	1148.0
25	1428.5
26	1914.0
27	2119.0
28	2355.0
29	2591.0
30	2842.0
31	3093.0
32	3122.0
33	3151.0
34	3432.5
35	3714.0
36	3845.0
37	3976.0
38	4780.0
39	5584.0
40	7089.0
41	8594.0
42	10065.0
43	11536.0
44	12015.5
45	12495.0
46	13407.0
47	14319.0
48	12529.0
49	10739.0
50	9690.5
51	8642.0
52	7247.5
53	5853.0
54	4873.0
55	3893.0
56	3220.5
57	2548.0
58	2181.0
59	1814.0
60	1539.0
61	1264.0
62	1016.5
63	769.0
64	585.0
65	401.0
66	294.5
67	188.0
68	161.5
69	135.0
70	114.0
71	93.0
72	71.5
73	50.0
74	45.5
75	32.5
76	24.0
77	19.0
78	14.0
79	10.0
80	6.0
81	3.0
82	0.0
83	0.0
84	0.0
85	0.5
86	1.0
87	1.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	128902.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.27697010131728
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.29123569325714	39.072318505531335
2	8.36285206001354	8.241920218460535
3	3.362773343409059	4.971218445020248
4	1.8388198806656275	3.6244588912507174
5	1.2075127127316236	2.975128392111837
6	0.8595853209275965	2.5414656095328234
7	0.6407531604716699	2.210206203162092
8	0.632881500023615	2.4949186203472404
9	0.5289755821092901	2.3459682549533754
>10	3.219509123254459	24.230035220555152
>50	0.022040649254553756	0.7051868861615801
>100	0.02518931343377572	2.434407534405983
>500	0.00629732835844393	2.30640331414563
>1k	0.0015743320896109826	1.8463639043614528
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2380	1.8463639043614528	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	959	0.7439760438162325	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG	806	0.6252812213929962	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	698	0.5414966408589471	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	510	0.3956494080774542	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	326	0.2529053079083335	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	325	0.2521295247552404	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	302	0.23428651223410032	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	269	0.20868566818202974	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	256	0.19860048719182014	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	225	0.17455120944593566	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	195	0.15127771485314426	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCT	163	0.12645265395416674	No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	163	0.12645265395416674	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTC	146	0.11326434035158492	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	138	0.10705807512684054	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	136	0.10550650882065445	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	135	0.1047307256675614	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14507144962839988	0.0
2	0.0	0.0	0.0	0.638469534995578	0.0
3	0.0	0.0	0.0	0.8254332748910025	0.0
4	0.0	0.0	0.0	1.2055670199065958	0.0
5	0.0	0.0	0.0	2.353726086484306	0.0
6	0.0	0.0	0.0	2.7734247723076444	0.0
7	0.0	0.0	0.0	3.2598408092969855	0.0
8	0.0	0.0	0.0	4.11630541031171	0.0
9	0.0	0.0	0.0	4.387053730741183	0.0
10	0.0	0.0	0.0	5.757862562256598	0.0
11	0.0	0.0	0.0	6.622860777955346	0.0
12	0.0	0.0	0.0	8.010736838838808	0.0
13	0.0	0.0	0.0	8.37535492079254	0.0
14	0.0	0.0	0.0	8.527408418798778	0.0
15	0.0	0.0	0.0	8.870304572465905	0.0
16	0.0	0.0	0.0	9.42421374377434	0.0
17	0.0	0.0	0.0	9.993638578144637	0.0
18	0.0	0.0	0.0	10.822950768801105	0.0
19	0.0	0.0	0.0	11.197654031745047	0.0
20	0.0	0.0	0.0	11.58554560829157	0.0
21	0.0	0.0	0.0	12.089028874648958	0.0
22	0.0	0.0	0.0	12.61113093668058	0.0
23	0.0	0.0	0.0	13.14099083024313	0.0
24	0.0	0.0	0.0	13.539743370932957	0.0
25	0.0	0.0	0.0	13.864796512078943	0.0
26	0.0	0.0	0.0	14.191401219531116	0.0
27	0.0	0.0	0.0	14.507144962839988	0.0
28	0.0	0.0	0.0	14.844610634435462	0.0
29	0.0	0.0	0.0	15.178973173418566	0.0
30	0.0	0.0	0.0	15.601774991854278	0.0
31	0.0	0.0	0.0	16.057159702719897	0.0
32	0.0	0.0	0.0	16.45048176133807	0.0
33	0.0	0.0	0.0	16.838373337884594	0.0
34	0.0	0.0	0.0	17.224713348124933	0.0
35	0.0	0.0	0.0	17.718111433492112	0.0
36	0.0	0.0	0.0	18.202975904175265	0.0
37	0.0	0.0	0.0	18.6451723014383	0.0
38	0.0	0.0	0.0	19.04159749266885	0.0
39	0.0	0.0	0.0	19.478363407860236	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAATC	25	3.8714956E-5	45.000004	11
ATGGGCG	50	2.1827873E-11	45.000004	5
CTGTCTA	20	7.009974E-4	45.000004	1
CACGGGC	20	7.009974E-4	45.000004	4
CAACGCA	20	7.009974E-4	45.000004	17
TTAGCGG	20	7.009974E-4	45.000004	2
ACGCAAG	20	7.009974E-4	45.000004	19
GGGTCCA	25	3.8714956E-5	45.000004	7
ATGGATT	20	7.009974E-4	45.000004	16
TTAGATG	20	7.009974E-4	45.000004	1
CGGTTTA	100	0.0	45.000004	1
TATGGCC	20	7.009974E-4	45.000004	10
CTGAGAG	20	7.009974E-4	45.000004	19
AACCAGA	20	7.009974E-4	45.000004	10
ATAGCCC	20	7.009974E-4	45.000004	31
GTTAGCG	20	7.009974E-4	45.000004	1
GGATATC	20	7.009974E-4	45.000004	8
CACGAGA	20	7.009974E-4	45.000004	14
CCTCGTC	30	2.1504802E-6	45.000004	36
ACTTACT	20	7.009974E-4	45.000004	38
>>END_MODULE