FastQCFastQC Report
Sat 18 Jun 2016
SRR3549439_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549439_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444180
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41350.9309289026971047No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15760.35481111261200415No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT8150.18348417308298437No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC7990.1798820298077356No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC7510.1690755999819893No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC6100.13733171236885947No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5360.12067179972083389No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG4800.10806429825746318No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4690.10558782475572966No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCA207.028606E-445.0000047
GCCTCCG207.028606E-445.00000444
CACAACG207.028606E-445.00000412
GCGTAAG207.028606E-445.0000041
CCCGATG207.028606E-445.00000428
CGACGGT850.045.028
GTCTCGC253.886928E-545.031
TGTATCG253.886928E-545.04
TTCGTAG351.2096825E-745.01
AGTATAC253.886928E-545.015
TATTGCG253.886928E-545.01
GGGACGT750.045.07
CCATCGA253.886928E-545.031
CTAGTCT253.886928E-545.028
TCTCGCG253.886928E-545.032
TCGTCCC253.886928E-545.038
CGGGACG253.886928E-545.06
TATCGCC253.886928E-545.06
ATCGCCA253.886928E-545.07
TCGCGCA253.886928E-545.034