##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549439_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	444180
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2775586473952	31.0	31.0	33.0	30.0	34.0
2	31.394486469449323	31.0	31.0	34.0	30.0	34.0
3	30.949169255707147	31.0	31.0	34.0	27.0	34.0
4	35.1098563645369	35.0	35.0	37.0	32.0	37.0
5	34.97808095817011	35.0	35.0	37.0	32.0	37.0
6	35.005484263136566	35.0	35.0	37.0	32.0	37.0
7	32.00960421450763	35.0	35.0	37.0	16.0	37.0
8	33.54248727993156	36.0	35.0	37.0	19.0	37.0
9	36.44371651132424	39.0	35.0	39.0	32.0	39.0
10	36.211806024584625	37.0	35.0	39.0	32.0	39.0
11	36.15956819307488	37.0	35.0	39.0	32.0	39.0
12	36.238401098653696	37.0	35.0	39.0	32.0	39.0
13	32.67776577063353	37.0	34.0	39.0	12.0	39.0
14	35.07579359719033	38.0	34.0	40.0	18.0	41.0
15	36.38211760997794	38.0	34.0	40.0	30.0	41.0
16	36.9628101220226	38.0	35.0	40.0	32.0	41.0
17	37.083884911522354	38.0	36.0	40.0	32.0	41.0
18	36.99486919717232	39.0	36.0	40.0	32.0	41.0
19	37.016349227790535	39.0	36.0	40.0	32.0	41.0
20	36.91007024179387	38.0	35.0	40.0	32.0	41.0
21	36.83033680039624	38.0	35.0	40.0	31.0	41.0
22	36.852048718987795	38.0	35.0	40.0	32.0	41.0
23	36.74703498581656	38.0	35.0	40.0	31.0	41.0
24	36.637529830249	38.0	35.0	40.0	31.0	41.0
25	36.44612544463956	38.0	35.0	40.0	31.0	41.0
26	36.321815480210724	38.0	35.0	40.0	31.0	41.0
27	36.3172475122698	38.0	35.0	40.0	30.0	41.0
28	36.18021297672115	38.0	35.0	40.0	30.0	41.0
29	36.189886982754736	38.0	35.0	40.0	30.0	41.0
30	36.003102345895805	38.0	35.0	40.0	30.0	41.0
31	35.84673330631726	38.0	35.0	40.0	30.0	41.0
32	35.65704219010311	38.0	34.0	40.0	29.0	41.0
33	35.43435319014814	38.0	34.0	40.0	27.0	41.0
34	35.20054707550993	38.0	34.0	40.0	26.0	41.0
35	34.99667927416813	38.0	34.0	40.0	25.0	41.0
36	34.80826466747715	38.0	34.0	40.0	24.0	41.0
37	34.62099824395516	38.0	33.0	40.0	24.0	41.0
38	34.48725741816381	38.0	33.0	40.0	23.0	41.0
39	34.273625557206536	38.0	33.0	40.0	23.0	41.0
40	34.177108379485794	37.0	33.0	40.0	23.0	41.0
41	34.084105542797964	37.0	33.0	40.0	22.0	41.0
42	34.05971227880589	37.0	33.0	40.0	22.0	41.0
43	34.025221756945385	37.0	33.0	40.0	22.0	41.0
44	33.87725921923545	37.0	33.0	40.0	21.0	41.0
45	33.84683911927597	37.0	33.0	40.0	21.0	41.0
46	33.683295060561036	37.0	33.0	40.0	20.0	41.0
47	33.421997838714034	37.0	32.0	40.0	20.0	41.0
48	33.302553019046336	37.0	32.0	40.0	19.0	41.0
49	33.20002476473502	37.0	32.0	40.0	18.0	41.0
50	32.952908730694766	36.0	31.0	40.0	18.0	41.0
51	30.977837813499033	34.0	28.0	38.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	3.0
9	12.0
10	23.0
11	13.0
12	6.0
13	6.0
14	6.0
15	19.0
16	36.0
17	97.0
18	186.0
19	406.0
20	711.0
21	1295.0
22	1953.0
23	3069.0
24	4685.0
25	6514.0
26	8125.0
27	9370.0
28	9965.0
29	11194.0
30	13640.0
31	16625.0
32	21349.0
33	28674.0
34	38658.0
35	46578.0
36	50635.0
37	63626.0
38	64481.0
39	42217.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.71331442208113	25.01103156378045	25.77401053626908	16.501643477869333
2	31.88639740645684	27.522175694538248	27.291413390967627	13.300013508037281
3	29.7118285379801	25.90053581881219	31.258949074699444	13.128686568508263
4	25.726282137872037	26.55747669863569	32.878337610878475	14.837903552613804
5	21.72520149488946	31.26592822729524	31.57683821874015	15.432032059075148
6	20.600882525102435	40.01643477869332	28.475617992705658	10.90706470349858
7	71.69278220541221	13.81534513035256	11.21189607816651	3.2799765860687105
8	76.92084290152641	5.293574676932775	12.400828492953307	5.384753928587509
9	70.07136746364087	7.8191273807915715	15.643883110450718	6.465622045116845
10	35.58557341618263	30.4358593363051	21.239137286685576	12.739429960826692
11	24.779593858345716	25.10693862848395	32.566076815705344	17.547390697464994
12	24.69584402719618	21.682876311405288	34.46305551803323	19.158224143365302
13	18.590436309604215	33.85294250078797	32.086991760097256	15.46962942951056
14	15.931829438515917	31.046872889369176	33.46683776847224	19.55445990364267
15	15.667972443603945	26.670043675987216	40.516682426043495	17.145301454365345
16	16.998063847989552	26.652258093565674	37.37516322211716	18.974514836327614
17	16.64617947678869	27.09824845783241	32.556846323562525	23.698725741816382
18	17.385969651942908	26.962717817101172	36.38007114232968	19.27124138862623
19	20.88995452294115	28.961907334864247	31.279886532486827	18.868251609707777
20	21.444909721284162	27.753838533927688	31.574586879193117	19.226664865595026
21	19.60511504345085	29.348012067179972	33.33423386915214	17.71263902021703
22	18.111126120041423	25.577918861722726	31.278085460849205	25.032869557386643
23	16.958440271961816	28.958080057634294	31.475753073078483	22.60772659732541
24	18.23562519699221	26.53473816921068	34.755504525192485	20.47413210860462
25	17.637894547255616	29.094961502093746	31.379846008374983	21.887297942275655
26	16.02571029762709	30.31631320635778	31.57391147732901	22.08406501868612
27	18.775946688279525	28.664280246746817	30.852582286460446	21.707190778513215
28	15.603809266513577	28.041109460128776	34.24039803683191	22.114683236525735
29	16.58336710342654	24.766986356882345	33.082984375703546	25.566662163987573
30	18.447251114413078	27.175469404295555	30.79832500337701	23.57895447791436
31	19.396190733486424	28.64446845873295	27.68899995497321	24.270340852807422
32	18.85812058174614	28.307442928542482	28.81557026430726	24.018866225404114
33	17.378540231437707	28.281327389796928	28.504435138907652	25.835697239857712
34	15.826691881669595	28.42563825476158	29.14471610608312	26.602953757485704
35	15.818136791390877	27.904002881714618	30.50745193390067	25.770408392993833
36	18.032329235895357	29.54365347381692	27.462064928632536	24.961952361655186
37	15.759601963168086	31.440857310099506	30.501598451078394	22.297942275654016
38	16.03696699536224	31.133999729839257	29.09406096627493	23.734972308523574
39	17.76351929397992	29.96105182583637	29.53397271376469	22.74145616641902
40	18.456256472601197	28.354721059030126	31.140303480570942	22.048718987797738
41	15.747669863568822	30.591652032959608	27.996532937097573	25.66414516637399
42	18.29686163267144	29.92548066099329	28.232923589535773	23.544734116799496
43	18.874555360439462	26.40956369039579	31.010851456616688	23.705029492548064
44	17.43054617497411	27.71939303885812	28.904948444324376	25.945112341843394
45	17.81687604124454	27.60795173128011	29.032824530595704	25.54234769687964
46	20.294475212751586	28.786077716241167	27.7473096492413	23.17213742176595
47	17.02800666396506	27.412985726507273	30.600207123238327	24.95880048628934
48	16.946733306317256	26.953712458913053	30.78504210004953	25.314512134720157
49	18.730244495474807	25.36561754243775	32.065153766491065	23.83898419559638
50	17.277004817866633	25.561484083029402	32.188752307623034	24.97275879148093
51	16.41204016389752	25.77243459858616	28.788329055788193	29.027196181728126
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	396.0
1	570.5
2	745.0
3	3830.5
4	6916.0
5	5996.0
6	5076.0
7	3969.5
8	2863.0
9	2809.0
10	2755.0
11	2918.0
12	3081.0
13	3017.5
14	2954.0
15	2915.0
16	2876.0
17	2792.5
18	2709.0
19	2592.5
20	2476.0
21	2513.5
22	2551.0
23	2641.5
24	2732.0
25	3458.5
26	4706.0
27	5227.0
28	6123.5
29	7020.0
30	8369.5
31	9719.0
32	10737.0
33	11755.0
34	12558.5
35	13362.0
36	14118.5
37	14875.0
38	16545.5
39	18216.0
40	21345.0
41	24474.0
42	28050.5
43	31627.0
44	35704.5
45	39782.0
46	45191.5
47	50601.0
48	51834.5
49	53068.0
50	48619.0
51	44170.0
52	36695.0
53	29220.0
54	23526.5
55	17833.0
56	14727.5
57	11622.0
58	9490.5
59	7359.0
60	6006.0
61	4653.0
62	3802.5
63	2952.0
64	2293.0
65	1634.0
66	1306.5
67	979.0
68	806.5
69	634.0
70	541.5
71	449.0
72	367.0
73	285.0
74	215.5
75	114.5
76	83.0
77	66.0
78	49.0
79	39.0
80	29.0
81	25.0
82	21.0
83	15.0
84	9.0
85	6.0
86	3.0
87	3.0
88	3.0
89	4.0
90	5.0
91	3.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	444180.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.581310761639344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.9819405059118	44.201004608337605
2	8.797386606856634	9.603457845582517
3	3.133602215136573	5.13108348923192
4	1.5422643984141302	3.367152496258176
5	0.9767245864210554	2.6655454089990647
6	0.6924064512708829	2.2675471014107966
7	0.5344758017943039	2.0420672882617876
8	0.45279828319317306	1.977145904584268
9	0.37864109950378666	1.8600054767230485
>10	2.4468842428214312	21.360601416203142
>50	0.04393245606482672	1.637165605185779
>100	0.016122002222501583	1.8584755483138922
>500	0.002015250277812698	0.7723849017672912
>1k	8.061001111250792E-4	1.2563629091407007
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4135	0.9309289026971047	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1576	0.35481111261200415	No Hit
CTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGCT	815	0.18348417308298437	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	799	0.1798820298077356	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTCTGC	751	0.1690755999819893	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCGTCTTC	610	0.13733171236885947	No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	536	0.12067179972083389	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTCAAATCGTATGCCG	480	0.10806429825746318	No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	469	0.10558782475572966	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3187896798595164	0.0
2	0.0	0.0	0.0	1.0916745463550812	0.0
3	0.0	0.0	0.0	1.476653608897294	0.0
4	0.0	0.0	0.0	2.055247872484128	0.0
5	0.0	0.0	0.0	3.6469449322346796	0.0
6	0.0	0.0	0.0	4.734341933450403	0.0
7	0.0	0.0	0.0	5.5810707370885675	0.0
8	0.0	0.0	0.0	7.7292989328650545	0.0
9	0.0	0.0	0.0	8.4416227655455	0.0
10	0.0	0.0	0.0	9.981764149669052	0.0
11	0.0	0.0	0.0	11.860056733756585	0.0
12	0.0	0.0	0.0	13.413481021207618	0.0
13	0.0	0.0	0.0	14.032599396641002	0.0
14	0.0	0.0	0.0	14.266288441622766	0.0
15	0.0	0.0	0.0	14.643838083659778	0.0
16	0.0	0.0	0.0	15.439911747489756	0.0
17	0.0	0.0	0.0	16.3514791300824	0.0
18	0.0	0.0	0.0	17.455536043946147	0.0
19	0.0	0.0	0.0	18.07510468728894	0.0
20	0.0	0.0	0.0	18.698725741816382	0.0
21	0.0	0.0	0.0	19.499527218695125	0.0
22	0.0	0.0	0.0	20.425052906479355	0.0
23	0.0	0.0	0.0	21.38907650051781	0.0
24	0.0	0.0	0.0	22.112431896978702	0.0
25	0.0	0.0	0.0	22.698905848980143	0.0
26	0.0	0.0	0.0	23.22887117835112	0.0
27	0.0	0.0	0.0	23.734296906659463	0.0
28	0.0	0.0	0.0	24.299383132964113	0.0
29	0.0	0.0	0.0	24.912422891620515	0.0
30	0.0	0.0	0.0	25.569138637489306	0.0
31	2.251339547030483E-4	0.0	0.0	26.177225449142238	0.0
32	4.502679094060966E-4	0.0	0.0	26.73420685307758	0.0
33	4.502679094060966E-4	0.0	0.0	27.341618262866405	0.0
34	4.502679094060966E-4	0.0	0.0	27.89049484443244	0.0
35	4.502679094060966E-4	0.0	0.0	28.535953892566077	0.0
36	4.502679094060966E-4	0.0	0.0	29.13210860461975	0.0
37	4.502679094060966E-4	0.0	0.0	29.693142419739747	0.0
38	4.502679094060966E-4	0.0	0.0	30.24719708226395	0.0
39	4.502679094060966E-4	0.0	0.0	30.795398261965868	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGCA	20	7.028606E-4	45.000004	7
GCCTCCG	20	7.028606E-4	45.000004	44
CACAACG	20	7.028606E-4	45.000004	12
GCGTAAG	20	7.028606E-4	45.000004	1
CCCGATG	20	7.028606E-4	45.000004	28
CGACGGT	85	0.0	45.0	28
GTCTCGC	25	3.886928E-5	45.0	31
TGTATCG	25	3.886928E-5	45.0	4
TTCGTAG	35	1.2096825E-7	45.0	1
AGTATAC	25	3.886928E-5	45.0	15
TATTGCG	25	3.886928E-5	45.0	1
GGGACGT	75	0.0	45.0	7
CCATCGA	25	3.886928E-5	45.0	31
CTAGTCT	25	3.886928E-5	45.0	28
TCTCGCG	25	3.886928E-5	45.0	32
TCGTCCC	25	3.886928E-5	45.0	38
CGGGACG	25	3.886928E-5	45.0	6
TATCGCC	25	3.886928E-5	45.0	6
ATCGCCA	25	3.886928E-5	45.0	7
TCGCGCA	25	3.886928E-5	45.0	34
>>END_MODULE