FastQCFastQC Report
Sat 18 Jun 2016
SRR3549438_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549438_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329432
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42351.2855460307438258No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCG18160.5512518516719687No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16870.5120935428252265No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGC14560.4419728502391996No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTC13650.41434954709924965RNA PCR Primer, Index 40 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA6920.21005852497632285No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6690.20307681099589595No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6200.18820272468976906No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG6090.18486364409043443No Hit
CGTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5430.16482916049442678No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG5410.16422205493091138No Hit
CGGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.1262779572111999No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA4150.1259744044294422No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCT3800.11535005706792298No Hit
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC3520.1068505791787076Illumina PCR Primer Index 8 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTC3420.10381505136113067No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3320.10077952354355375No Hit
GAACTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCT3310.10047597076179605TruSeq Adapter, Index 19 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAG351.2084092E-745.0000041
GCAATAG351.2084092E-745.0000041
CGGTTTA1750.045.01
TATGGGC750.045.04
TCGATTG207.025952E-445.01
GCGGGAG850.045.05
TCGTCCC406.7902874E-945.038
GGCGACT551.8189894E-1245.010
CCGCATG207.025952E-445.01
CGCATGG502.1827873E-1144.9999962
TACGGGT253.8847276E-544.9999964
GACGGGT302.1607702E-644.9999964
TTAACGG302.1607702E-644.9999962
TTACCGG253.8847276E-544.9999962
TAGGGCA800.042.18755
GGCCTAT750.042.08
TTAATCG650.041.5384620
CGGGATC650.041.538466
TACGGCT2450.041.326537
ACGGGTA603.6379788E-1241.2499965
Produced by FastQC (version 0.11.5)